Paraoxonase-1 activity and the levels of lipids and lipid peroxidation markers in arterial versus venous blood samples in coronary angiography patients

Abstract

A b s t r a c tIntroduction: The percutaneous catheterization of various arteries is used in visualization of coronary arteries. Aim: We aimed to determine whether arterial blood samples withdrawn from femoral arteries during standard Judkin's technique in patients evaluated with coronary angiography can also be used to determine some biochemical parameters.Material and methods: In 50 controls (25 males and 25 females) and 73 coronary artery disease (CAD) patients (10 females and 63 males) paraoxonase-1 (PON1) activity, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG) levels were measured using colorimetric methods. Lipid peroxidation marker levels (conjugated dienes (CD) and thiobarbituric acid-reactive substances (TBARS)) were measured manually.Results: There was no difference in lipid and lipid peroxidation marker levels, PON1 activity and TC/HDL-C, LDL-C/HDL-C and PON1/HDL-C ratios between arterial and venous blood samples. LDL-C, CD and TBARS levels and TC/HDL-C and LDL-C/HDL-C ratios were significantly lower in both arterial and venous blood samples of controls compared with CAD patients. Paraoxonase-1 activity, HDL-C level and PON1/HDL-C ratio were higher in controls than CAD patients. On multiple logistic regression analysis, risk factors associated with CAD were found to be the levels of arterial CD, venous CD, arterial TBARS and arterial LDL-C/HDL-C ratios in CAD patients.Conclusions: Our study might indicate that arterial blood samples can also be used as well as venous samples to determine these parameters. On the other hand, elevated arterial lipid peroxides are associated with cardiovascular complications, presumably by decreasing PON1 activity.Key words: paraoxonase, artery, vein, lipids, lipid peroxidation, coronary artery disease S t r e s z c z e n i e Wstęp: Cewnikowanie przezskórne jest wykorzystywane w obrazowaniu tętnic wieńcowych. Cel: Określenie, czy próbki krwi tętniczej pobrane z tętnicy udowej u pacjentów poddawanych koronarografii standardową techniką Judkinsa mogą zostać użyte do oceny niektórych parametrów biochemicznych.Materiał i metody: Przy użyciu metod kalorymetrycznych u 50 osób z grupy kontrolnej (25 mężczyzn i 25 kobiet) oraz u 73 pacjentów (10 kobiet i 63 mężczyzn) z chorobą wieńcową oceniono aktywność paraoksonazy 1 (PON1), stężenie cholesterolu całkowitego (total cholesterol -TC), cholesterolu lipoprotein o niskiej gęstości (low-density lipoprotein cholesterol -LDL-C), cholesterolu lipoprotein o bardzo niskiej gęstości (very low-density lipoprotein cholesterol -VLDL-C), cholesterolu lipoprotein o wysokiej gęstości (highdensity lipoproteid cholesterol -HDL-C) oraz triglicerydów (triglycerides -TG). Poziomy markerów peroksydacji lipidów [sprzężone dieny (conjugated dienes -CD) oraz związki reagujące z kwasem tiobarbiturowym (thiobarbituric acid-reactives substances -TBARS)] określono ręcznie.

NLRP3 and downstream cytokine expression elevated in the monocytes of patients with coronary artery disease

“…IL-1β and IL-18 were considered as a master switch of inflammation, which acted as mediators to promote the cascade release of other cytokines. IL-1β and IL-18 have been previously implicated in the pathogenesis of atherosclerosis [ 12 – 16 ].…”

Paraoxonase 1: The Lectin-Like Oxidized LDL Receptor Type I and Oxidative Stress in the Blood of Men with Type II Obesity

“…In the 90s, it was described as having antioxidant capabilities. It allowed the hypothesis that PON1 is responsible for the protection of LDLs against oxidation, which is important in the pathogenesis of atherosclerosis [18]. PON1 is a glycoprotein with a mass of 43-47 kDa, in the center of which there are two calcium ions, performing both the stabilizing and catalytic ion function.…”