Paraoxonase-1 gene in patients with chronic obstructive pulmonary disease investigation Q192R and L55M polymorphisms

Abstract

BACKGROUND:The effect of increased oxidative stress on the development of chronic obstructive pulmonary disease (COPD) is well known. One of the antioxidative systems against oxidative stress in human body is paraoxonase (PON) enzyme that protects low density lipoproteins (LDL) against oxidation. This study aimed to explore the polymorphisms on PON1, Q192R, L55M genes of patients with COPD.METHODS: DNAs extraction was obtained from blood samples of 50 patients diagnosed with COPD and 50 patients as a control group who were presented to emergency clinic. Genotypes were obtained with polymerase chain reaction (PCR) and AIw I and Hsp92II restriction enzymes were used for Q192R and L55M polymorphisms, respectively. Analysis of data was done with the Chi-square test and Fisher's exact test. RESULTS:A statistically significant difference in Q192R polymorphism was found between the COPD patients and the control group (P=0.05). There was no statistically signifi cant difference in L55M polymorphisms between the patient and control groups (P>0.05). Q192R polymorphism was signifi cantly correlated with the PON1 gene and cigarette smoking; however other risk factors did not show any significant correlation with this polymorphism. Though L55M polymorphism was signifi cantly correlated with family history and tuberculosis, there was no signifi cant correlation with other risk factors. CONCLUSION:We believe that more studies are needed to study the correlation of L55M polymorphism with other factors.

Identification of Prognostic Value of Rs3735590 Polymorphism in 3’-Untranslated Region (3’-UTR) of Paraoxonase 1 (PON-1) in Chronic Obstructive Pulmonary Disease Patients who Received Coronary Artery Bypass Grafting (CABG)

“…Meanwhile, PON1 is an enzyme involved in the lipid metabolism, while a low level of PON1 activity is associated with a decreased concentration of high-density lipoproteins (HDL) that suggests a greater risk of CHD and a poor prognosis in patients receiving surgical interventions [7]. Consistent with such results, the variants located in PON1 gene have been found to be associated with the risk of COPD [8].…”

Q192R polymorphism in the PON1 gene and familial hypercholesterolemia in a Saudi population

“… 50 PON1 protein acts as a lipophilic antioxidant in both the liver and serum by binding to HDL and plays a protective role against oxidative stress in the lungs. 51 Recently, meta-analyses have become important approaches to confirm the accuracy of findings in the global population and to facilitate complex analyses. Unfortunately, few meta-analyses of the Q192R polymorphism have been performed in patients with FH, 52 , 53 and no meta-analyses have described the few case-control studies of the Q192R variant in patients with FH.…”

Paraoxonase 1 and Chronic Obstructive Pulmonary Disease: A Meta-Analysis

“…Of the 119 initially identified articles, 100 articles were excluded after screening of titles and abstracts. After screening of full-texts, six studies were excluded because six studies did not focus on PON1 activity of patients with COPD [ 22 , 23 , 24 , 25 , 26 , 27 ]. One study [ 28 ] was excluded because it included the same population as another study [ 29 ].…”