Paraquat exposure-induced Parkinson’s disease-like symptoms and oxidative stress in Drosophila melanogaster: Neuroprotective effect of Bougainvillea glabra Choisy

Soares, Jefferson J.; Rodrigues, Daniela Teixeira; Gonçalves, Mayara B.; Lemos, Maurício C.; Gallarreta, Mariana S.; Bianchini, Matheus Chimelo; Gayer, Mateus Cristofari; Puntel, Robson Luiz; Roehrs, Rafael; Denardin, Elton Luís Gasparotto

doi:10.1016/j.biopha.2017.08.073

Cited by 52 publications

(39 citation statements)

References 33 publications

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“…The flower MeOH extract was found to contain the highest amounts of gallic acid (2.39 µg/g), catechin (6.31 µg/g), and rutin (1.26 µg/g), while coumaric acid was below the limit of detection (BLD) (<0.1 µg/mL). These results are in agreement with previous studies on the isolation of different parts of this plant, reporting compounds like gallic acid, rutin, and coumaric acid [11,14]. Likewise, the flower DCM extract contained catechin (0.26 µg/g), sinapinic acid (0.23 µg/g), 2,3-diMeO benzoic acid (0.52 µg/g), and benzoic acid (0.26 µg/g).…”

Section: Hplc-pda Polyphenolic Quantificationsupporting

confidence: 92%

“…The TICs for this extract are depicted in Figure 3. previous studies on the isolation of different parts of this plant, reporting compounds like gallic acid, rutin, and coumaric acid [11,14]. Likewise, the flower DCM extract contained catechin (0.26 μg/g), sinapinic acid (0.23 μg/g), 2,3-diMeO benzoic acid (0.52 μg/g), and benzoic acid (0.26 μg/g).…”

Section: Uhplc-ms Secondary Metabolite Analysismentioning

confidence: 68%

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HPLC–PDA Polyphenolic Quantification, UHPLC–MS Secondary Metabolite Composition, and In Vitro Enzyme Inhibition Potential of Bougainvillea glabra

Saleem,

Htar,

Naidu

et al. 2020

Plants

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The plants of the Bougainvillea genus are widely explored regarding nutritive and medicinal purposes. In this study, dichloromethane (DCM) and methanol (MeOH) extracts of Bougainvillea glabra (Choisy.) aerial and flower parts were analyzed for high-performance liquid chromatography with photodiode array detection (HPLC–PDA), ultra-high-performance liquid chromatography–mass spectrometry (UHPLC–MS) phytochemical composition, and enzyme inhibition potential against key enzymes involved in diabetes (α-amylase), skin problems (tyrosinase), and inflammatory disorders (lipoxygenase (LOX)). HPLC–PDA quantification revealed the identification of nine different polyphenolics, amongst which both flower extracts were richest. The flower MeOH extract contained the highest amount of catechin (6.31 μg/g), gallic acid (2.39 μg/g), and rutin (1.26 μg/g). However, none of the quantified compounds were detected in the aerial DCM extract. UHPLC–MS analysis of DCM extracts revealed the tentative identification of 27 secondary metabolites, where the most common belonged to terpenoid, alkaloid, and phenolic derivatives. Similarly, for enzyme inhibition, all the extracts presented moderate activity against tyrosinase and α-amylases, whereas, for LOX, both methanolic extracts showed higher percentage inhibition compared with DCM extracts. Based on our findings, B. glabra could be regarded as a perspective starting material for designing novel pharmaceuticals.

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Section: Hplc-pda Polyphenolic Quantificationsupporting

confidence: 92%

Section: Uhplc-ms Secondary Metabolite Analysismentioning

confidence: 68%

HPLC–PDA Polyphenolic Quantification, UHPLC–MS Secondary Metabolite Composition, and In Vitro Enzyme Inhibition Potential of Bougainvillea glabra

Saleem,

Htar,

Naidu

et al. 2020

Plants

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“…Accordingly, previous studies identified myriad compounds that counteract PQ. Bougainvillea glabra leaf extract, Decalepis hamiltonii root extract, quercetin, and curcumin protect against PQ-induced mortality, locomotor dysfunction and oxidative damage, respectively (Park, Jung, Ahn, & Kwon, 2012;Jahromi, Haddadi, Shivanandappa, & Ramesh, 2013;Soares et al, 2017) In this work, we used the ultrasonic extraction technique to more efficiently extract the natural antioxidants present in S. cumini leaves. UAE is an important technique for extracting valuable compounds from vegetal materials.…”

Section: Discussionmentioning

confidence: 99%

Protective role of <i>Syzygium cumini</i> leaf extracts against paraquat-induced oxidative stress in superoxide-dismutase-deficient<i> Saccharomyces cerevisiae</i> strains

Soares

Rosa

Motta

et al. 2019

Acta Sci. Biol. Sci.

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The aim of this study was to evaluate the protective effect of three different extracts prepared from Syzygium cumini leaves against paraquat-induced toxicity in Saccharomyces cerevisiae strains deficient in superoxide dismutase (SOD). Additionally, the extracts phenolic and flavonoid contents, in vitro antioxidant activity, and phytochemical composition (using high-pressure liquid chromatography) were determined. Bioactive compounds from S. cumini leaves were extracted with infusion (traditional method) or ultrasound (aqueous or hydroalcoholic). Compared to the infusion extract, the ultrasound extracts exhibited a greater protective capacity against paraquat toxicity in the yeast cells as well as higher antioxidant activity. These results may be directly related to the higher phenolic and flavonoid contents in these extracts, since they are recognized as having high antioxidant actions.

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“…The important mechanism of PQ toxicity in different experimental models (including plants, bacteria and animals) is oxidative stress ( 17 ). Antioxidants have become potential therapeutic agents.…”

Section: Discussionmentioning

confidence: 99%

Dynamics study on the role of curcumin on TGF‑β1 expression and pathological changes in acute paraquat poisoned rats

Chen

2018

Exp Ther Med

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Role of curcumin on TGF-β1 expression and pathological changes in acute paraquat (PQ) poisoned rats were investigated. Forty-eight SD rats were divided into three groups: control group, PQ group, and PQ + curcumin group, with 16 rats in each group. PQ group rats received the lavagation of PQ every day, PQ + curcumin group was given the lavagation of curcumin treatment on the basis of the PQ group. Control group received the lavagation of physiological saline. The body weight of rats was recorded every day. Six rats were randomly selected on the 1st, 3rd and 7th day after treatment from each group and sacrificed by cervical dislocation. The blood and liver tissues of each rat were collected. The morphology change of the liver tissue was observed by hematoxylin and eosin (H&E) staining. The expression level of TGF-β1 in the liver tissue was detected by western blot analysis and RT-qPCR. The blood samples were sent to the inspection section of the hospital for the detection of reactive oxygen species (ROS), alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA) and superoxide dismutase (SOD). On the 1st day after poisoning, the liver cells of PQ rats had obvious edema; obvious fatty degeneration was observed on the 3rd day; and large number of cavities appeared on the 7th day due to necrosis. For the PQ + curcumin group, liver cell edema appeared on the 3rd day, and mild swelling of liver cells was observed on the 7th day. Compared with the control group, the expression of TGF-β1 was increased in the PQ group. The TGF-β1 level in PQ + curcumin group rats reached the peak on the 3rd day, then decreased, and it was lower than those in PQ group. The level of ROS, ALT, AST, MDA of the rats in PQ + curcumin group reached the highest value on the 3rd day, while the level of SOD reached the lowest value; furthermore, the level of ROS, ALT, AST, MDA was lower than that in PQ group, while the level of SOD was higher than that of the PQ group. The results showed that curcumin can effectively inhibit the expression of TGF-β1, prevent PQ-induced liver cell oxidative damage and play an important role in the protection of liver function.

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Paraquat exposure-induced Parkinson’s disease-like symptoms and oxidative stress in Drosophila melanogaster: Neuroprotective effect of Bougainvillea glabra Choisy

Cited by 52 publications

References 33 publications

HPLC–PDA Polyphenolic Quantification, UHPLC–MS Secondary Metabolite Composition, and In Vitro Enzyme Inhibition Potential of Bougainvillea glabra

HPLC–PDA Polyphenolic Quantification, UHPLC–MS Secondary Metabolite Composition, and In Vitro Enzyme Inhibition Potential of Bougainvillea glabra

Protective role of <i>Syzygium cumini</i> leaf extracts against paraquat-induced oxidative stress in superoxide-dismutase-deficient<i> Saccharomyces cerevisiae</i> strains

Dynamics study on the role of curcumin on TGF‑β1 expression and pathological changes in acute paraquat poisoned rats

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