Parasite load and its association with disease outcome in cutaneous leishmaniasis in Sri Lanka

Riyal, F. H.; Samaranayake, T. N.; Ganeshalingam, J.; Priyani, A. A. H.; Munidasa, Deepani; Karunaweera, Nadira D.

doi:10.4038/sljid.v11i0.8368

Cited by 1 publication

(2 citation statements)

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“…Positive CD1a staining of amastigotes of Old World leishmaniasis by L. major and L. tropica has been previously reported [69]. In 2023, Riyal et al, identified a similar staining pattern in L. donovani causing CL for the first time, with a sensitivity of 74% [61]. While fluorescence in situ hybridization (FISH) is not a common approach in diagnosing CL, applying Leishmania genus-specific FISH probes on FFPE tissue has been shown to improve sensitivity over conventional staining (Table 3) [70].…”

Section: Supplementary Tests To Histopathologymentioning

confidence: 84%

“…Histiocytes and lymphocytes are common cell types seen in the dermal infiltrate. Varying degrees of granuloma formation have been reported in different settings, with higher parasite loads negatively associated with well-defined granulomas [59,61]. The spectrum of histological changes seen in CL patients ranging from diffuse infiltrates of parasitized macrophages to well-formed granulomas often resembles those reported for leprosy [62].…”

Section: Histopathologymentioning

confidence: 97%

See 1 more Smart Citation

Diagnostic Tools for Cutaneous Leishmaniasis Caused by Leishmania donovani: A Narrative Review

Piyasiri,

Dewasurendra,

Samaranayake

et al. 2023

Diagnostics

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Leishmaniasis, a neglected tropical disease, encompasses a spectrum of clinical conditions and poses a significant risk of infection to over one billion people worldwide. Visceral leishmaniasis (VL) in the Indian sub-continent (ISC), where the causative parasite is Leishmania donovani, is targeted for elimination by 2025, with some countries already reaching such targets. Other clinical phenotypes due to the same species could act as a reservoir of parasites and thus pose a challenge to successful control and elimination. Sri Lanka has consistently reported cutaneous leishmaniasis (CL) due to L. donovani as the primary disease presentation over several decades. Similar findings of atypical phenotypes of L. donovani have also been reported from several other countries/regions in the Old World. In this review, we discuss the applicability of different methods in diagnosing CL due to L. donovani and a comprehensive assessment of diagnostic methods spanning clinical, microscopic, molecular, and immunological approaches. By incorporating evidence from Sri Lanka and other regions on L. donovani-related CL, we thoroughly evaluate the accuracy, feasibility, and relevance of these diagnostic tools. We also discuss the challenges and complexities linked to diagnosing CL and review novel approaches and their applicability for detecting CL.

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Section: Supplementary Tests To Histopathologymentioning

confidence: 84%