2015
DOI: 10.1128/iai.00415-15
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Parasite Manipulation of the Invariant Chain and the Peptide Editor H2-DM Affects Major Histocompatibility Complex Class II Antigen Presentation during Toxoplasma gondii Infection

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Cited by 15 publications
(19 citation statements)
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“…Our observations are in agreement with others. Indeed, it has been shown the ability of T gondii infection to downregulate MHC II gene expression and antigen presentation on macrophages and DCs, though this has only previously been reported in vitro . Our results suggest a pregnancy‐related mechanism interfering with MHC II expression, and therefore, with antigen presentation during T gondii infection.…”
Section: Discussionmentioning
confidence: 49%
“…Our observations are in agreement with others. Indeed, it has been shown the ability of T gondii infection to downregulate MHC II gene expression and antigen presentation on macrophages and DCs, though this has only previously been reported in vitro . Our results suggest a pregnancy‐related mechanism interfering with MHC II expression, and therefore, with antigen presentation during T gondii infection.…”
Section: Discussionmentioning
confidence: 49%
“…Bone marrow-derived macrophage differentiation. Bone marrow-derived macrophages (BMDM) were obtained by differentiating precursor cells from murine bone marrow (101). Briefly, mice were euthanized by CO 2 asphyxiation, hind legs were collected in Hanks' balanced salt solution (HBSS) (100 U/ml penicillin, 100 g/ml streptomycin, 4.2 mM sodium bicarbonate), and marrow was flushed out of bones.…”
Section: Methodsmentioning
confidence: 99%
“…Measurement of parasite replication by qPCR. Parasite replication in vitro was assessed by extracting genomic DNA (gDNA), followed by measuring the amplification of the T. gondii B1 gene by qPCR, as described previously (101). Briefly, BMDM cultures were plated in 12-well plates overnight; serum starved for 2 h in the presence of DMSO (vehicle), 20 nM rapamycin, or 200 nM Torin-1; and then inoculated with T. gondii tachyzoites (RH and ME49 strains) at an MOI of 1:20.…”
Section: Methodsmentioning
confidence: 99%
“…Recently, a pioneering study has investigated the metabolic needs and capabilities of T. gondii using a combined computational and experimental approach 10 . The interplay between T. gondii parasites and their hosts has been studied for decades using targeted approaches, such as the analysis of mutants or host immunological responses 11 12 . Although much has been learned from such studies, they focus on individual pathways and fail to reveal the global effects of infection on the host’s metabolism.…”
mentioning
confidence: 99%