Parasite OMICS, the grand challenges ahead

Mitreva, Makedonka

doi:10.3389/fpara.2022.995302

Cited by 3 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, the presence of immunomodulatory proteins in all stages might mask stage-specific variaGons in their abundance, requiring targeted analyses for accurate comparisons. It is important to highlight the relaGvely small number of idenGfied proteins might not capture the full complexity of the proteomes [68][69][70][71][72]. Focusing on a broader range of proteins or employing targeted approaches might reveal significant differences between stages.…”

Section: Discussionmentioning

confidence: 99%

Characterizing Excretory-Secretory Products Proteome Across Larval Development Stages inAscaris suum

Castañeda,

Adeniyi-Ipadeola,

et al. 2024

Preprint

View full text Add to dashboard Cite

IntroductionAscaris lumbricoidesandAscaris suumare parasitic nematodes that primarily infest the small intestines of humans and pigs, respectively. Ascariasis poses a significant threat to human health and swine health. UnderstandingAscarislarval development is crucial for developing novel therapeutic interventions that will prevent ascariasis in both humans and pigs. This study aimed to characterize the excretory-secretory (ES) proteome of differentAscaris suumlarval stages (L3-egg, L3-lung, L3-trachea) to identify potential targets for intervention to preventAscaris-induced global morbidity.MethodsStage-specific larvae were isolated, cultured in vitro and ES-product was collected. Third-stageAscarislarvae (L3) were isolated from embryonated eggs (L3-egg), isolated from the lungs of Balb/c mice infected withAscaris suumeggs at day 8 post infection (L3-lungs) and isolated from the trachea of Balb/c mice infected withAscaris suumeggs at day 12 post infection (L3-trachea). ES products were obtained by culturing larvae. Proteomic analysis was conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and bioinformatic tools including MaxQuant, Perseus, and Andromeda, following a detailed protocol available on GitHub. The analysis encompassed peptide identification, scoring, and quantification against an organism-specific database, with subsequent quality control, correlation assessment, and differential abundance determination using the Amica algorithm.ResultsA total of 58 unique proteins were identified in the ES products. Fourteen proteins were common across all stages, while others were stage-specific. Principal component analysis revealed distinct protein profiles for each stage, suggesting qualitatively different proteomes. Gene ontology analysis indicated stage-specific GO enrichment of specific protein classes, such as nuclear proteins in L3-egg ES products and metabolic enzymes in L3-lung and L3-trachea ES products.DiscussionThis study revealed stage-specific differences in the composition ofAscarisES products. Further investigation into the functional roles of these proteins and their interactions with host cells is crucial for developing novel therapeutic and diagnostic strategies against ascariasis.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterizing Excretory-Secretory Products Proteome Across Larval Development Stages inAscaris suum

Castañeda,

Adeniyi-Ipadeola,

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…As -omics techniques enable the broad discovery of temporally-resolved molecular alterations underlying host-parasite interactions, disease progression, and therapeutic targets during different stages of parasite infection ( Hasin et al, 2017 ; Mitreva, 2022 ). Considerable progress in parasitology has been made through generating and interrogating omics data ( Gardinassi et al, 2018 ; Liu et al, 2021 ; Athieniti and Spyrou, 2023 ).…”

Section: Exploring Host-parasite Interactions: Integrating Omics Disc...mentioning

confidence: 99%

Advancing vaccine development against Opisthorchis viverrini: A synergistic integration of omics technologies and advanced computational tools

Kafle,

Ojha

2024

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

Parasite-microbiota interactions: a pathway to innovative interventions for Chagas disease, leishmaniasis, and ascariasis

Ramírez,

Castañeda,

Weatherhead

et al. 2024

Future Microbiology

View full text Add to dashboard Cite

Parasite OMICS, the grand challenges ahead

Abstract: August CITATION Mitreva M () Parasite OMICS, the grand challenges ahead.

Cited by 3 publications

References 26 publications

Characterizing Excretory-Secretory Products Proteome Across Larval Development Stages inAscaris suum

Characterizing Excretory-Secretory Products Proteome Across Larval Development Stages inAscaris suum

Advancing vaccine development against Opisthorchis viverrini: A synergistic integration of omics technologies and advanced computational tools

Parasite-microbiota interactions: a pathway to innovative interventions for Chagas disease, leishmaniasis, and ascariasis

Contact Info

Product

Resources

About