Parasitic larvae in bronchoalveolar lavage fluid in a non‐immunocompromised patient

Kumar, Tarun; Prasad, Tanya; Nigam, Jitendra Singh; Sinha, Ruchi; Bhadani, Punam Prasad

doi:10.1111/cyt.13200

Cited by 1 publication

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“…This study aimed to gain a comprehensive understanding of the microbiome composition in the BALF of children with severe pneumonia in the PICU. Although uncommon, protozoa such as parasites may also represent the "potential" pathogenic microbial composition in BALF ( Van den Steen et al, 2010;Deroost et al, 2013;Wihokhoen et al, 2016;Kumar et al, 2022). Therefore, after metagenomic sequencing, the sequenced reads were aligned to bacterial, viral, and fungal databases, as well as to protozoan databases, and the "potential" protozoan composition was obtained.…”

Section: Correlation Of Abundance Of Potentially Pathogenic Bacteria ...mentioning

confidence: 99%

Metagenomic next-generation sequencing of bronchoalveolar lavage fluid from children with severe pneumonia in pediatric intensive care unit

Zhang

Liu

Wang

et al. 2023

Front. Cell. Infect. Microbiol.

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BackgroundSevere pneumonia due to lower respiratory tract infections (LRTIs) is a significant cause of morbidity and mortality in children. Noninfectious respiratory syndromes resembling LRTIs can complicate the diagnosis and may also make targeted therapy difficult because of the difficulty of identifying LRTI pathogens. In the present study, a highly sensitive metagenomic next-generation sequencing (mNGS) approach was used to characterize the microbiome of bronchoalveolar lavage fluid (BALF) in children with severe lower pneumonia and identify pathogenic microorganisms that may cause severe pneumonia. The purpose of this study was to use mNGS to explore the potential microbiomes of children with severe pneumonia in a PICU.MethodsWe enrolled patients meeting diagnostic criteria for severe pneumonia admitted at PICU of the Children’s Hospital of Fudan University, China, from February 2018 to February 2020. In total, 126 BALF samples were collected, and mNGS was performed at the DNA and/or RNA level. The pathogenic microorganisms in BALF were identified and correlated with serological inflammatory indicators, lymphocyte subtypes, and clinical symptoms.ResultsmNGS of BALF identified potentially pathogenic bacteria in children with severe pneumonia in the PICU. An increased BALF bacterial diversity index was positively correlated with serum inflammatory indicators and lymphocyte subtypes. Children with severe pneumonia in the PICU had the potential for coinfection with viruses including Epstein–Barr virus, Cytomegalovirus, and Human betaherpesvirus 6B, the abundance of which was positively correlated with immunodeficiency and pneumonia severity, suggesting that the virus may be reactivated in children in the PICU. There was also the potential for coinfection with fungal pathogens including Pneumocystis jirovecii and Aspergillus fumigatus in children with severe pneumonia in the PICU, and an increase in potentially pathogenic eukaryotic diversity in BALF was positively associated with the occurrence of death and sepsis.ConclusionsmNGS can be used for clinical microbiological testing of BALF samples from children in the PICU. Bacterial combined with viral or fungal infections may be present in the BALF of patients with severe pneumonia in the PICU. Viral or fungal infections are associated with greater disease severity and death.

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Section: Correlation Of Abundance Of Potentially Pathogenic Bacteria ...mentioning

confidence: 99%

Metagenomic next-generation sequencing of bronchoalveolar lavage fluid from children with severe pneumonia in pediatric intensive care unit

Zhang

Liu

Wang

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

Parasitic larvae in bronchoalveolar lavage fluid in a non‐immunocompromised patient

Cited by 1 publication

References 3 publications

Metagenomic next-generation sequencing of bronchoalveolar lavage fluid from children with severe pneumonia in pediatric intensive care unit

Metagenomic next-generation sequencing of bronchoalveolar lavage fluid from children with severe pneumonia in pediatric intensive care unit

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