Parasitism Genes: What They Reveal about Parasitism

Davis, Eric; Hussey, R. S.; Baum, Thomas J.

doi:10.1007/7089_2008_29

Cited by 23 publications

(24 citation statements)

References 137 publications

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“…Indirect approaches, including the generation of promoter:: GUS transgenic plants or the examination of genes or promoters not originally cloned from nematode-infested tissues, were also conducted (reviewed in Gheysen and Fenoll 2002) . For the expression of genes in nematodes, studies have been carried out to identify and characterize gene transcripts in an attempt to understand the location and timing of gene expression at the various stages of nematode development and during the parasitism process (Davis et al 2008). …”

Section: Early Methods Analyzing Gene Expression During Nematode Paramentioning

confidence: 99%

“…With only three gland cells among over 950 cells in an intact nematode, the targeted isolation of glands using microaspiration techniques is required to find potential genes essential for nematode parasitism. Several studies using the microaspiration approach identified over 50 different putative secreted parasitism genes from the esophageal glands of root-knot and cyst nematodes (Davis et al 2008 ). In contrast, Roze et al (2008) isolated only eight secreted gland expressed genes after screening more than 12,000 expressed sequence tags (ESTs) generated from whole nematodes (representing three developmental stages).…”

Section: Temporal and Spatial Analysis Of Tissues For Gene Expressionmentioning

confidence: 98%

“…For instance, esophageal glands of nematodes are thought to be one of the organs within the nematode that produce parasitism molecules that are secreted and injected into host cells for the initiation and development of nematode feeding sites (Davis et al 2008 ). With only three gland cells among over 950 cells in an intact nematode, the targeted isolation of glands using microaspiration techniques is required to find potential genes essential for nematode parasitism.…”

Section: Temporal and Spatial Analysis Of Tissues For Gene Expressionmentioning

confidence: 99%

“…Following the induction of feeding sites, nematodes resume development and undergo multiple morphological changes from a motile infective juvenile to a mature male or egg-laying adult female. The enormous developmental changes over the life cycle of nematodes are likely reflected in dramatic changes in gene expression (Davis et al 2008).…”

Section: Temporal and Spatial Analysis Of Tissues For Gene Expressionmentioning

confidence: 99%

“…A significant amount of evidence on changes in gene expression within plant-parasitic nematodes comes from the studies of esophageal glands and the genes that are expressed within the three gland cells. Davis et al (2008) provide an in depth analysis of gene expression in the esophageal glands of root-knot or cyst nematodes.…”

Section: Early Gene Expression Analyses Of Plant-parasitic Nematodesmentioning

confidence: 99%

See 4 more Smart Citations

Transcriptomic Analysis of Nematode Infestation

Li¹,

Fester²,

Christopher³

et al. 2008

Plant Cell Monographs

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The establishment and maintenance of nematode feeding sites and subsequent nematode development are associated with significant changes in expression level of both plant and nematode genes. Analysis of the changes in transcript abundance during feeding site establishment and function provides insight into the complexity of plant-nematode interactions and may lead to the discovery of novel strategies for managing nematodes. Early techniques of gene discovery had identified a small cadre of genes induced in the plant host or invading nematode. More recently, the adoption of high-throughput gene expression tools such as microarrays has allowed the identification of hundreds of both plant and nematode genes that are regulated over the course of nematode infestation. Initially, microarray studies were used to examine gene expression in whole infested roots or nematode feeding site-enriched tissues. More recently, the combination of laser-assisted microdissection of nematode feeding sites and subsequent transcriptomic analyses using microarrays has provided an unprecedented view of gene expression within the feeding site itself. This chapter is intended to present a broad overview of the knowledge gained to date about techniques that have been used to identify differentially regulated genes of both plant and nematode during the plant-nematode interaction and recent methodological improvements crucial for future studies.

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Section: Early Methods Analyzing Gene Expression During Nematode Paramentioning

confidence: 99%

Section: Temporal and Spatial Analysis Of Tissues For Gene Expressionmentioning

confidence: 98%

Section: Temporal and Spatial Analysis Of Tissues For Gene Expressionmentioning

confidence: 99%

Section: Temporal and Spatial Analysis Of Tissues For Gene Expressionmentioning

confidence: 99%

Section: Early Gene Expression Analyses Of Plant-parasitic Nematodesmentioning

confidence: 99%

See 3 more Smart Citations

Transcriptomic Analysis of Nematode Infestation

Li¹,

Fester²,

Christopher³

et al. 2008

Plant Cell Monographs

View full text Add to dashboard Cite

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Development of the Root-Knot Nematode Feeding Cell

Berg¹,

Fester²,

Taylor³

2008

Plant Cell Monographs

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Plant Infection by Root-Knot Nematode

Bird¹,

Opperman²,

Williamson³

2008

Plant Cell Monographs

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Plant-parasitic nematodes, particularly the sedentary endoparasitic forms, are cosmopolitan pests, collectively causing over $100 billion in annual crop loss worldwide. In the past decade, significant progress has been made in identifying genes and their products that define key aspects of the host-parasite interface, including enzymes and proteins with direct roles in virulence and resistance. However, little remains known about how a host is identified or how the development of the nematode is coupled to establishment of the parasitic interaction. Here, we consider the role of signaling molecules and their interplay with nematode development from hatch through primary interaction with the plant.

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Parasitism Genes: What They Reveal about Parasitism

Cited by 23 publications

References 137 publications

Transcriptomic Analysis of Nematode Infestation

Transcriptomic Analysis of Nematode Infestation

Development of the Root-Knot Nematode Feeding Cell

Plant Infection by Root-Knot Nematode

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