“…The parasitological methods are commonly based on the parasite's detectability in blood smears or lymph node, skin, spleen, or bone morrow cytology (ALVAR et al, 2004;NOLI & SARIDOMICHELAKIS, 2014). However, collection of most of these specimens is not easy, and the sensitivity of these tests can vary from 20% to 80% (SARIDOMICHELAKI et al, 2009, SOLANO-GALLEGO et al, 2009, SILVA et al, 2014. One interesting alternate option for the direct diagnosis of Leishmania sp.…”