Parasitological and immunological evaluation of cattle experimentally infected with Trypanosoma vivax

Bassi, Paula Boeira; Araújo, Fernanda Fortes de; Garcia, Guilherme Caetano; Silva, Marcos Vinícius da; Oliveira, Carlo José Freire; Bittar, Eustáquio Resende; Gomes, Matheus de Souza; Amaral, Laurence Rodrigues do; Silva, Matheus Fernandes Costa e; Nascentes, Gabriel Antônio Nogueira; Rodrigues, Virmondés; Martins‐Filho, Olindo Assis; Araújo, Márcio Sobreira Silva; Bittar, Joely Ferreira Figueiredo

doi:10.1016/j.exppara.2018.01.010

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…Studies with experimental infection of T. vivax in cattle have already demonstrated the infection of this protozoan when animals are infected subcutaneously, intravenously (Fidelis Junior et al, 2016;Bassi et al, 2018;Bastos et al, 2020b), intradermally and intramuscularly (Bastos et al, 2020b). The reuse of hypodermic needles, although not recommended, is a quite common practice in several countries (USDA, 2011).…”

Section: Discussionmentioning

confidence: 99%

How many cattle can be infected by Trypanosoma vivax by reusing the same needle and syringe, and what is the viability time of this protozoan in injectable veterinary products?

et al. 2021

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It was investigated how many cattle become infected with Trypanosoma vivax by subcutaneous (SC), intramuscular (IM) and intravenous (IV) routes, using the same syringe and needle from an animal with acute T. vivax infection. Besides, the T. vivax viability in 109 injectable veterinary drugs (antibiotics, antiparasitics, reproductive hormones, vitamin complex and derivatives, vaccines, anaesthetics, anti-inflammatory/antipyretics, antitoxics). In the field assay, four groups were performed: T01, T02 and T03 animals that received saline solution with the same syringe and needle contaminated with T. vivax via SC, IM and IV routes, respectively, and T04 control animals that received only saline solution with the same syringe and needle IV. In the laboratory, drugs had their pH measured and T. vivax viability verified. The number of cattle infected with T. vivax via SC (3/20) was lower (P ≤ 0.05) compared to via IM (9/20), which was lower (P ≤ 0.05) compared to IV (15/20). The solution pH did not influence T. vivax viability. In 44% (48/109) of the products, T. vivax remained viable regardless of time, stooding out that in 100% of oxytocins the protozoan was verified, at some evaluation times. The mean of T. vivax quantified in foot-and-mouth and brucellosis vaccines and in doramectin-based products were higher (P ≤ 0.05) than found in blood + saline solution.

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Section: Discussionmentioning

confidence: 99%

How many cattle can be infected by Trypanosoma vivax by reusing the same needle and syringe, and what is the viability time of this protozoan in injectable veterinary products?

et al. 2021

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“…Macrophages and lymphocyte subsets expressing CD163 or WC1, respectively, play a role against certain parasite infections (e.g. Theileria parva [66], Leishmania braziliensis [67], Trypanosoma vivax [68], or Neospora caninum [69]), but no direct interaction with parasite helminth structures has been previously reported.…”

Section: Discussionmentioning

confidence: 99%

The ectodomains of the lymphocyte scavenger receptors CD5 and CD6 interact with tegumental antigens from Echinococcus granulosus sensu lato and protect mice against secondary cystic echinococcosis

et al. 2018

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BackgroundScavenger Receptors (SRs) from the host’s innate immune system are known to bind multiple ligands to promote the removal of non-self or altered-self targets. CD5 and CD6 are two highly homologous class I SRs mainly expressed on all T cells and the B1a cell subset, and involved in the fine tuning of activation and differentiation signals delivered by the antigen-specific receptors (TCR and BCR, respectively), to which they physically associate. Additionally, CD5 and CD6 have been shown to interact with and sense the presence of conserved pathogen-associated structures from bacteria, fungi and/or viruses.Methodology/Principal findingsWe report herein the interaction of CD5 and CD6 lymphocyte surface receptors with Echinococcus granulosus sensu lato (s.l.). Binding studies show that both soluble and membrane-bound forms of CD5 and CD6 bind to intact viable protoscoleces from E. granulosus s.l. through recognition of metaperiodate-resistant tegumental components. Proteomic analyses allowed identification of thioredoxin peroxidase for CD5, and peptidyl-prolyl cis-trans isomerase (cyclophilin) and endophilin B1 (antigen P-29) for CD6, as their potential interactors. Further in vitro assays demonstrate that membrane-bound or soluble CD5 and CD6 forms differentially modulate the pro- and anti-inflammatory cytokine release induced following peritoneal cells exposure to E. granulosus s.l. tegumental components. Importantly, prophylactic infusion of soluble CD5 or CD6 significantly ameliorated the infection outcome in the mouse model of secondary cystic echinococcosis.Conclusions/SignificanceTaken together, the results expand the pathogen binding properties of CD5 and CD6 and provide novel evidence for their therapeutic potential in human cystic echinococcosis.

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“…O teste de ELISA indireto é bastante utilizado por ser simples e útil para avaliação de um número grande de animais (OSÓRIO et al, 2008;GONZATTI et al, 2013). A RIFI tem mostrado boa sensibilidade, mesmo assim recomenda-se a associação de dois métodos diagnósticos, haja visto que a presença de anticorpos não significa necessariamente que a infecção esteja ativa no animal (BASSI et al, 2018).…”

Section: Sinais Clínicosunclassified

Trypanosoma vivax: uma breve revisão / Trypanosoma vivax: a brief review

Castelli¹,

Silva²,

Costa³

et al. 2021

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RESUMOTrypanosoma vivax é o protozoário causador da tripanossomose bovina, doença que afeta ruminantes em geral e que pode provocar quadros de anemia, queda reprodutiva, aborto, diminuição da produção leiteira e morte dos animais levando a grandes perdas econômicas. O presente estudo visa descrever as características clínicas e epidemiológicas, do vetor, formas de transmissão e diagnóstico e controle da tripanossomose bovina causada por T. vivax no Brasil.

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Parasitological and immunological evaluation of cattle experimentally infected with Trypanosoma vivax

Cited by 7 publications

References 27 publications

How many cattle can be infected by Trypanosoma vivax by reusing the same needle and syringe, and what is the viability time of this protozoan in injectable veterinary products?

How many cattle can be infected by Trypanosoma vivax by reusing the same needle and syringe, and what is the viability time of this protozoan in injectable veterinary products?

The ectodomains of the lymphocyte scavenger receptors CD5 and CD6 interact with tegumental antigens from Echinococcus granulosus sensu lato and protect mice against secondary cystic echinococcosis

Trypanosoma vivax: uma breve revisão / Trypanosoma vivax: a brief review

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