Parasitological and serological detection of Trypanosoma evansi on Bali cattle at the Pesanggaran slaughterhouse, Denpasar, Indonesia using hematological profile

Abstract

Abstract. Apsari IAP, Suratma NA, Swacita IBN, Soma IG, Sari TK, Putra IPC, Sudipa PH. 2024. Parasitological and serological detection of Trypanosoma evansi on Bali cattle at the Pesanggaran slaughterhouse, Denpasar, Indonesia using hematological profile. Biodiversitas 25: 1057-1062. Bali cattle (Bos sondaicus Blyth, 1842 syn. Bos javanicus domesticus Wilckens, 1905) are vulnerable to blood protozoan infections like Trypanosoma evansi. Indonesia's warm and moist environment provides ideal conditions for blood-sucking flies such as Stomoxys calcitrans, Haematobia irritans, and Hipobosca sp. to thrive and spread. These flies act as carriers of T. evansi, which can then infect Bali cattle. This study aims to detect the presence of T. evansi in Bali cattle. Blood samples were collected from 275 Bali cattle at the Pesanggaran slaughterhouse, Denpasar City, Bali, Indonesia. The samples were examined using the Giemsa-Staining Blood Smear (GSBS) method and hematology profile examination using Hematology Analyzer RT-7600 for Vet. Serum samples were also collected for serologic examination using the Card Agglutination Test for Trypanosomiasis (CATT) method. The hematological profile data obtained were analyzed descriptively, and the Mann-Whitney U test was used to determine the significance of hematological values between female and male cattle seropositive to T. evansi. The results showed that the T. evansi parasite was not detected in the blood smear examination. However, the serological detection with CATT revealed that 6.54% (18/275) of Bali cattle were positive for T. evansi. The hematological profiles of T. evansi seropositive cattle remained within the normal range, except for neutrophil percentage. White Blood Cell (WBC), Hemoglobin (Hb) levels, and hematocrit (PCV) values of male cattle were significantly (P?0.05) lower than those of females. Therefore, based on serological examination, it can be concluded that the protozoa T. evansi is present in 6.54% of Bali cattle at the Pesanggaran slaughterhouse.

Investigation into Trypanosoma evansi Infection in Horses in East Sumba-Indonesia

“…Clinical signs of Surra are not always sufficiently specific for confirmation clinical diagnoses, so that laboratory tests are required to support definitive diagnosis . The diagnosis of trypanosomiasis can be based on finding the parasite itself, although with limited sensitivity and specificity compared to serological assay (Apsari et al 2024), so that detecting its molecular properties with more sensitivity and high specificity were advised . The specific potency of every test should be evaluated carefully for effective application based on the different epidemiological conditions.…”

“…Detection of T. evansi through blood smear is very dependent on the amount of parasitemia that occurs. Serologically, ELISA and CATT both give good results in cattle, buffalo and horses (Laha and Sasmal 2009;Apsari et al 2024). For field investigations in horses, it was suggested to use serological diagnosis with Card Agglutination Test for T. evansi (CATT) because it provides more sensitive, it also showed a higher prevalence for a wide range of many animal species than other traditionally used diagnostic methods (Tehseen et al 2017;Junco et al 2024).…”