Parasitological diagnosis of canine visceral leishmaniasis: Is intact skin a good target?

Madeira, M.F.; Figueiredo, Fabiano Borges; Pinto, A.G.S.; Nascimento, Lílian Dias; Furtado, Marina Carvalho; Mouta-Confort, Eliame; Paula, C. C. De; Bogio, Alessandra; Gomes, Marta C.; Bessa, A.M.S.; Passos, Sônia Regina Lambert

doi:10.1016/j.rvsc.2009.03.008

Cited by 46 publications

(43 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, skin, bone marrow, blood and spleen in agreement with previous studies (MADEIRA et al, 2009;ALMEIDA et al, 2013), and the spleen showed a higher percentage of L. chagasi DNA detection than the bone marrow. According to REIS et al (2013), the spleen is considered one of the major organs affected by Leishmania sp.…”

Section: -----------------------------------------Sampledsupporting

confidence: 93%

“…The observation that 96.04% of PCR positives were associated with seropositive dogs confirms the occurrence of the infection, despite of the possibility of cross-reactivity in serological tests as recommended by the Ministry of Health (BRAZIL, 2006;MADEIRA et al, 2009). This finding also enabled the identification of the L. chagasi species in domestic reservoirs in Jaciara, which were previously found only in the vector (BRITO et al, 2014b).…”

Section: Palavras-chave: Cão Leishmaniose Visceral Pcr Distintos Tsupporting

confidence: 55%

See 1 more Smart Citation

Leishmania chagasi in dogs from the city of Jaciara, Mato Grosso, Brazil

et al. 2015

View full text Add to dashboard Cite

show abstract

Section: -----------------------------------------Sampledsupporting

confidence: 93%

Section: Palavras-chave: Cão Leishmaniose Visceral Pcr Distintos Tsupporting

confidence: 55%

Leishmania chagasi in dogs from the city of Jaciara, Mato Grosso, Brazil

et al. 2015

View full text Add to dashboard Cite

show abstract

“…In two studies conducted in the Municipality of Rio de Janeiro, MADEIRA et al 16,17 isolated Leishmania from 18/20 and from 31/39 animals, respectively, using skin fragments obtained from dogs euthanized because of a suspicion of CVL for parasitological confirmation. In another study, the same authors reported a rate of isolation of 78.7% when skin samples from 394 dogs were evaluated 15 . A similar percentage (83%) has been reported by BARROUIN-MELO et al 2 who analyzed spleen samples from seroreactive dogs.…”

Section: Discussionmentioning

confidence: 95%

“…Clinical specimens for the diagnosis of CVL by parasitological examination can be obtained by aspiration of bone marrow, spleen, liver and lymph nodes and, in some cases, by a biopsy of intact skin 15,16 , lesion, or viscera 2,3 .…”

Section: Introductionmentioning

confidence: 99%

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Figueiredo

Madeira

Nascimento

et al. 2010

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYThe Brazilian Ministry of Health recommends the culling and euthanasia of dogs with a positive serological test for canine visceral leishmaniasis (CVL). In the Municipality of Rio de Janeiro, the technique used for the diagnosis of CVL is the indirect fluorescent antibody test (IFAT), using blood samples eluted on filter paper (eluate). A dog survey was conducted over a period of one year in the region of Carapiá, in order to evaluate the diagnosis of CVL in this region. All animals underwent clinical examination, and blood samples (serum and eluate) were collected for analysis by enzyme immunoassay (ELISA) and IFAT. A skin biopsy was obtained for parasitological examination (culture). A total of 305 animals were studied and Leishmania chagasi was isolated from nine animals. Sensitivity and specificity were 100% and 96.6% for ELISA, respectively, 100% and 65.5% for IFAT (cut-off at a 1:40 dilution), 100% and 83.4% for IFAT (cut-off at a 1:80 dilution), and 22.2% and 97.0% for eluate IFAT. In conclusion, ELISA was the best tool for the diagnosis of CVL among the serological techniques tested. The present results suggest the need for a better evaluation of filter paper IFAT as the only diagnostic method for CVL in the Municipality of Rio de Janeiro.

show abstract

“…Therefore, a variety of serological, parasitological, and molecular laboratory methods have been developed to detect infection by L. infantum in dogs (10,11). Serological assays, PCR, and parasitological culture are the most sensitive methods for the diagnosis of L. infantum infection (12)(13)(14)(15)(16)(17)(18). However, these three methods do not allow visualization of the intact amastigotes within the tissue and correlation of the parasites with associated lesions, which is possible by histopathology (HP) and immunohistochemistry (IHC) (19).…”

mentioning

confidence: 99%

Sensitivity and Specificity of In Situ Hybridization for Diagnosis of Cutaneous Infection by Leishmania infantum in Dogs

et al. 2013

View full text Add to dashboard Cite

An accurate diagnosis of infection by Leishmania infantum in dogs is fundamental for the control of zoonotic visceral leishmaniasis (VL). Histopathology (HP) and immunohistochemistry (IHC) are frequently used for the histological diagnosis of L. infantum in dogs but have shown limited accuracy. To improve the sensitivity and specificity of the histological diagnosis of VL, we evaluated automated in situ hybridization (ISH) using a generic probe for Leishmania and a specific probe for L. infantum in surgical skin biopsy specimens of dogs. The ISH results were compared with those of HP and IHC, using parasitological culture as the reference standard. Skin samples from 51 dogs with cutaneous L. infantum infection and 51 noninfected dogs were randomly selected from samples of dogs from various cities in Brazil where canine VL is endemic. These samples were processed for parasitological culture, HP, IHC, and ISH using both probes. The sensitivities of ISH using the specific probe, ISH using the generic probe, IHC, and HP were, respectively, 74.5%, 70.6%, 69.5%, and 57.6%. The specificity of both ISH probes tested was 100%, and there was no cross-hybridization of the generic and specific probes with selected pathogenic fungi and protozoa. The specific probe discriminated L. infantum from the other species of Leishmania that infect dogs in the New World. ISH is highly sensitive and specific for the diagnosis of L. infantum in histologic samples of skin from infected dogs and can be used on routine biopsy material to make a diagnosis of leishmaniasis.

show abstract

Parasitological diagnosis of canine visceral leishmaniasis: Is intact skin a good target?

Cited by 46 publications

References 19 publications

Leishmania chagasi in dogs from the city of Jaciara, Mato Grosso, Brazil

Leishmania chagasi in dogs from the city of Jaciara, Mato Grosso, Brazil

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Sensitivity and Specificity of In Situ Hybridization for Diagnosis of Cutaneous Infection by Leishmania infantum in Dogs

Contact Info

Product

Resources

About