Parathyroid hormone administration may modulate periodontal tissue levels of interleukin‐6, matrix metalloproteinase‐2 and matrix metalloproteinase‐9 in experimental periodontitis

supporting

confidence: 49%

Intermittent Parathyroid Hormone Administration Improves Periodontal Healing in Rats

Vasconcelos

Marques²,

Benatti

et al. 2014

“…MMP9 activity increases significantly in the gingival tissues of patients with periodontitis 24 . Such an MMP9 increase was observed in ligature 16,25 but not in GAV models 26 . CATB is involved in physiologic and pathologic tissue remodeling and cell apoptosis 27 .…”

Section: Methodsmentioning

confidence: 99%

“…Using imaging software, ‡‡‡‡ TRAP‐positive cells were counted on the alveolar bone crest surface at the palatal root and the mesial and distal furcation aspects of the first molar using standardized views. The number of TRAP‐positive cells was divided by the bone soft connective tissue linear surface length 25 …”

Section: Methodsmentioning

confidence: 99%

Periodontal and Systemic Responses in Various Mice Models of Experimental Periodontitis: Respective Roles of Inflammation Duration and Porphyromonas gingivalis Infection

Saadi-Thiers

Huck

Simonis

et al. 2013

“…21 Aliquots of gingival tissue homogenates containing the same protein amount were run under non-reducing conditions without heat denaturation onto 10% sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE) co-polymerized with 1.6 mg/mL of gelatin **** as substrate. After SDS-PAGE, the gels were washed twice in 2% Triton X-100 for 30 min each, and then the gels were incubated overnight in activation buffer (50 mM Tris-HCl, pH 7.4, 5 mM CaCl 2 ) for 16 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Fluoxetine Inhibits Inflammatory Response and Bone Loss in a Rat Model of Ligature‐Induced Periodontitis

Branco-de-Almeida

Franco

Castro

et al. 2012

Background Fluoxetine, a selective serotonin reuptake inhibitor, has recently been found to possess anti-inflammatory properties. The present study investigated the effects of fluoxetine on inflammatory tissue destruction in a rat model of ligature-induced periodontitis (PD). Methods Male Wistar rats were randomly assigned into three groups (n=10 animals/group): 1) Control rats (without ligature); 2) rats with ligature + placebo (saline; oral gavage); 3) rats with ligature + fluoxetine (20 mg/kg/day in saline; oral gavage). Histological analyses were performed on the furcation region and mesial of mandibular first molars of rats sacrificed at 15 days after ligature-induced PD. Reverse transcriptase-polymerase chain reaction (RT-PCR) and zymography were performed to analyze the mRNA expression of interleukin (IL)-1β, cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9 and inducible nitric oxide synthase (iNOS), and the MMP-9 activity, respectively, in gingival tissues samples. Results Compared to the ligature + placebo group, alveolar bone loss was reduced in the fluoxetine group (P < 0.05), and the integrity of collagen fibers in the gingival tissue was maintained. Moreover, in gingival tissue sampled 3 days after ligature attachment, fluoxetine administration reduced IL-1β and COX-2 mRNA expression. Fluoxetine down-regulated MMP-9 activity, without affecting MMP-9 mRNA expression induced by ligature, compared to the ligature + placebo group (P < 0.05). These data suggested that fluoxetine suppressed proinflammatory responses, as well as proteolytic enzyme activity, induced by ligature. Conclusions In the present study, fluoxetine suppressed the inflammatory response and protected against periodontal bone resorption and destruction of collagen fibers, suggesting that fluoxetine can constitute a promising therapeutic approach for periodontal diseases.