Parathyroid hormone bioassay using human kidney cortical cells in primary culture

Zonefrati, Roberto; Brandi, Maria Luisa; Rotella, Carlo Maria; Selli, Cesare; Toccafondi, R

doi:10.1530/acta.0.1000398

Cited by 5 publications

(2 citation statements)

References 14 publications

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“…In agreement with that observed by Marx and Aurbach [6], in human plasma membranes the potency of PTH on kidney adenylate cyclase-cAMP system, described in detail in a previous paper [11], is much higher when compared to that of equal doses of sCT.…”

Section: Discussionsupporting

confidence: 91%

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Salmon calcitonin and cGMP production by human kidney: Studies in vivo and in vitro

et al. 1983

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In order to evaluate whether or not the action of salmon calcitonin (sCT) at the kidney level could be mediated through specific receptors for the hormone, we have studied the effects of sCT infusions on urinary excretion of cyclic nucleotides in humans. Parallel in vitro studies have been conducted by evaluating the effects of sCT on cyclic nucleotide levels in primary cultures of cortical and medullary human kidney cells. In vivo experiments showed that sCT induced an increase in cGMP in human urine, which was rapid and short-lasting, being superimposable on the increase of urinary excretion of calcium and magnesium. The increase of inorganic phosphate urinary excretion was delayed and appeared to parallel that of urinary cAMP. On the other hand, our in vitro experiments showed that sCT stimulated the guanylate cyclase-cGMP system of human kidney cortical cells at nanomolar concentrations, while higher concentrations of the hormone were required to activate the adenylate cyclase-cAMP system. In addition, sCT was not able to significantly modify the cellular levels of either nucleotide in human kidney medullary cells. Present data demonstrated a direct effect of sCT on human kidney cortical cGMP production, while the efficacy of sCT on the kidney cortex adenylate cyclase-cAMP system appears to be delayed and/or reduced.

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Section: Discussionsupporting

confidence: 91%

“…Statistical analysis was performed by one-way variance analysis. Human PTH (hPTH 1-84) was purified from culture medium of hyperplastic parathyroid ceils in primary culture [11]. …”

Section: Cyclic Nucleotide and Dna Measurementmentioning

confidence: 99%

Salmon calcitonin and cGMP production by human kidney: Studies in vivo and in vitro

et al. 1983

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Development of Simple and Sensitive System for Detection of Bone Seeking Substances Employing Chick Embryo Tibiae

Kurokawa

Kawakubo

Yoneda

et al. 1989

New Actions of Parathyroid Hormone

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Effect of prostaglandin E2 on adenylate cyclase system in human kidney cell cultures: interactions with parathormone and arginine-vasopressin

Rotella

Brandi

Toccafondi

1983

J Endocrinol Invest

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We have used human kidney cortical and medullary cells in primary culture as an experimental model, in order to study the effect of prostaglandin E2 on the adenylate cyclase-cyclic AMP system at renal level, in comparison with that of parathormone and desamino 1-D-arginine 8-vasopressin. Prostaglandin E2 is a more potent stimulator of cyclic AMP accumulation in cortical cells than in medullary ones, the minimal effective dose being 3 nM in the cortex and 20 microM in the medulla. The maximal response was obtained with 30 microM in cortical cells and with 0.3 mM in medullary ones. The effects of combining submaximal and maximal active doses of the three hormones were always additive when tested on cortical cells. On the contrary, prostaglandin E2, at doses effective on the medullary cells adenylate cyclase system, produced an increase of cyclic AMP accumulation significantly lower than expected when incubated with submaximal and maximal amounts of desamino 1-D-arginine 8-vasopressin. Present data provide evidence for the existence of distinct receptors for parathormone, arginine-vasopressin and prostaglandin E2 at cortical level; the possible role of prostaglandin E2 in the counter-regulation of arginine-vasopressin action on medullary cells in unlikely, in view of the high prostaglandin E2 concentration which is necessary to exert this effect.

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Parathyroid hormone bioassay using human kidney cortical cells in primary culture

Cited by 5 publications

References 14 publications

Salmon calcitonin and cGMP production by human kidney: Studies in vivo and in vitro

Salmon calcitonin and cGMP production by human kidney: Studies in vivo and in vitro

Development of Simple and Sensitive System for Detection of Bone Seeking Substances Employing Chick Embryo Tibiae

Effect of prostaglandin E2 on adenylate cyclase system in human kidney cell cultures: interactions with parathormone and arginine-vasopressin

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