Paratransgenic Control of Leishmaniasis: New Developments

Hurwitz, Ivy; Forshaw, Adam; Yacisin, Kari; Ramalho-Ortigão, Marcelo; Satoskar, Abhay R.; Durvasula, Ravi

doi:10.1007/978-1-4614-9108-8_3

Cited by 5 publications

(3 citation statements)

References 57 publications

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“…The first study of the possibility of genetically manipulating sand fly commensal bacteria capable of effectively colonising the insect and remaining permanent throughout the life-cycle was conducted by Hurtwitz et al [ 330 , 331 ] with B. subtilis , previously isolated from P. argentipes. For this work, B. subtilis expressing a GFP reporter gene was added in the diet and offered to fourth stage sand fly larvae; the insects were then dissected and the midgut homogenates analysed for colonisation by recombinant bacteria using PCR and colony-forming units.…”

Section: Paratransgenesis In Different Groups Of Vectorsmentioning

confidence: 99%

Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors

et al. 2022

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This article presents an overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors. It first briefly summarises some of the disease-causing pathogens vectored by insects and emphasises the need for innovative control methods to counter the threat of resistance by both the vector insect to pesticides and the pathogens to therapeutic drugs. Subsequently, the state of art of paratransgenesis is described, which is a particularly ingenious method currently under development in many important vector insects that could provide an additional powerful tool for use in integrated pest control programmes. The requirements and recent advances of the paratransgenesis technique are detailed and an overview is given of the microorganisms selected for genetic modification, the effector molecules to be expressed and the environmental spread of the transgenic bacteria into wild insect populations. The results of experimental models of paratransgenesis developed with triatomines, mosquitoes, sandflies and tsetse flies are analysed. Finally, the regulatory and safety rules to be satisfied for the successful environmental release of the genetically engineered organisms produced in paratransgenesis are considered. Graphical Abstract

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Section: Paratransgenesis In Different Groups Of Vectorsmentioning

confidence: 99%

Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors

et al. 2022

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“…Notwithstanding, this method was proven useful for specifically delivering the EGFP- or GFP-expressing bacteria. Similar approaches may be used to deliver selected microbes to sand fly larvae in paratransgenic applications to control sand fly populations [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Infection and Immune Responses in Lutzomyia longipalpis Sand Fly Larvae Midgut

et al. 2015

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The midgut microbial community in insect vectors of disease is crucial for an effective immune response against infection with various human and animal pathogens. Depending on the aspects of their development, insects can acquire microbes present in soil, water, and plants. Sand flies are major vectors of leishmaniasis, and shown to harbor a wide variety of Gram-negative and Gram-positive bacteria. Sand fly larval stages acquire microorganisms from the soil, and the abundance and distribution of these microorganisms may vary depending on the sand fly species or the breeding site. Here, we assess the distribution of two bacteria commonly found within the gut of sand flies, Pantoea agglomerans and Bacillus subtilis. We demonstrate that these bacteria are able to differentially infect the larval digestive tract, and regulate the immune response in sand fly larvae. Moreover, bacterial distribution, and likely the ability to colonize the gut, is driven, at least in part, by a gradient of pH present in the gut.

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“…Even as singular or a hybrid peptide, it was studied against Leishmania spp. and Trypanosoma spp., and researchers found direct antimicrobial and indirect immunomodulatory results [27][28][29][30].Another promising result was found about HIV. Dosedependently, melittin was found to prevent synthesis of group-specific antigen (gag)/DNA polymerase (pol) and intracellularly reduce Gag antigen and HIV-1 mRNA levels.…”

Section: Major Bioactive Substancesmentioning

confidence: 99%

Bee venom: A medical perspective

Sığ

Güney

Sig

et al. 2019

Turkish Journal of Clinics and Laboratory

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Apitherapy is a complementary medical technique that has an old history and is applied in various diseases worldwide. Apitherapeutical applications are not treatment methods by themself, but they can be substantial parts of multidisciplinary approaches. One of them, bee venom therapy (BVT), is a currently-applied method worldwide. Bee venom (BV) includes several substances such as peptides, phospholipids, bioactive amines, amino acids, sugars, pheromones, enzymes and minerals.Studies on whole BV and its singular components indicated that they have a huge potential in anti-inflammatory, anti-arthritis, anti-nociceptive, neuroprotective, anti-tumoral, anti-microbial, anti-diabetic and anti-rheumatic activities. Results of in vivo studies against arthritis, Parkinson's and Alzheimer's disease and cancer are very promising, and also in vitro results indicating other activities such as antimicrobial effect are observed. Although mechanisms of action and many bioactive substances still remain unclear, beneficial effects and potential utilities in certain medical conditions are obvious. It seems bioactive components of BV may open new doors in treatment of various diseases.

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Paratransgenic Control of Leishmaniasis: New Developments

Cited by 5 publications

References 57 publications

Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors

Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors

Bacterial Infection and Immune Responses in Lutzomyia longipalpis Sand Fly Larvae Midgut

Bee venom: A medical perspective

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