Parentage assignment in hybrid abalones (<i>Haliotis rufescens</i> × <i>Haliotis discus hannai</i>) based on microsatellite DNA markers

Yang

J World Aquaculture Soc

et al. 2016

Japanese flounder, Paralichthys olivaceus, is an economically important marine fish species in Asia. A suite of 18 microsatellite markers chosen from published genetic linkage maps was used to carry out parentage assignments of 188 hatchery‐reared juveniles from a small number of breeders. The probabilities of exclusion for the 18 microsatellite markers were 0.604–0.913, and the effectiveness of combined probability of exclusion reached 100% when using the eight microsatellite markers with higher Excl 1 probabilities. The cultured and wild stocks (WSs) were differentiated in a release‐recapture population based on these markers. Of the 321 recaptured offspring, 28.34% were assigned to their parental pairs in our broodstock, whereas the remaining offspring could not be traced back to a possible sire or dam. Significant reduction in genetic diversity of the cultured stock (CS) had not been found compared with that of the WS. The results suggest that CSs released into the wild will not adversely affect the genetic structure of natural populations. Our results demonstrate that these markers provide an efficient tool for parentage assignments and genetic analysis of Japanese flounder.

Section: Discussionmentioning

confidence: 99%

Use of Microsatellite DNA Profiling to Identify Japanese Flounder, Paralichthys olivaceus of Hatchery Origin

Yang

J World Aquaculture Soc

et al. 2016

“…In general, the fishery and culture of this species has traditionally depended on the natural spat settlement, and the current artificial genetic improvements of this species are limited (Guo, Ford & Zhang ; Wang, Guo, Zhang & Zhang ; Guo ). Crossbreeding, which effectively combines the desirable characteristics of one stock of species with those of another, is regarded as an effective way to produce genetic improvement of marine shellfish (Hedgecock, McGoldrick & Bayne ; Hulata ; Hedgecock, McGoldrick, Manahan, Vavra, Appelmans & Bayne ; Toro, Thompson & Innes ; Zheng, Zhang, Guo & Liu ; Zhang, Liu, Zhang & Wang ; Ahmed, Koike, Strüssmann, Yamasaki, Yokota & Watanabe ; You, Ke, Luo & Wang ; Luo, Ke, You, Wang & Chen ; Gu, Shi, Wang & Wang ; Zheng, Xu & Zhang ; Zhang, Wang, Yan, Yu, Kong, Liu, Li, Li & Guo ; Zheng, Zhang, Sun, Liu & Liu ; Zhang, Zhang, Wang, Yan & Yu ; Lafarga‐de la Cruz, Aguilar‐Espinoza & Gallardo‐Escárate ), and has been documented in scallops (Cruz & Ibarra ; Zheng et al . , ; Zhang et al .…”

Section: Introductionmentioning

confidence: 99%

Survival and growth of reciprocal crosses between two stocks of the Hong Kong oyster C rassostrea hongkongensis ( L am & M orton, 2003) in southern China

Zhang

et al. 2016

Aquac Res

The Hong Kong oyster, Crassostrea hongkongensis, is one of the most economically important and well-known oyster species in southern China. To explore the possibility of improving the performance of the Hong Kong oyster, complete diallel crosses between two oyster stocks, the Zhuhai stock (Z: fast growth line F4) and the Maowei Sea stock (M: resistant line F3), were conducted using pooled gametes. Three replicates, each consisting of two pure stocks (ZZ and MM) and two reciprocal crosses (ZM and MZ), were successfully generated. High fertilization rate and hatching level were observed among all the experimental groups, suggesting that there was no sperm-egg recognition barrier between geographic populations. Reciprocal crosses had higher survival rate compared to the pure stock crosses and the rate of survival increased with progeny growth. Growth heterosis became obvious both in larval and adult stages, and was primarily influenced by the egg origin and mating strategy at the larval stage. Also, the phenotypic traits of all progeny differed amongst the culture sites, suggesting a significant environmental effect. The Zhuhai site was more suitable for oyster aquaculture than the Maowei Sea site. Thus, our results demonstrated that crossbreeding between Zhuhai and Maowei Sea stocks of Hong Kong oyster to produce superior heterosis represents a promising means to improve the fishery yield of this species in southern China.

J World Aquaculture Soc

“…Ke and You () conducted crossbreeding of a Taiwanese population ( ♀ ) × a Japanese population (♂) of Haliotis diversicolor aquatili and obtained a new strain that had significantly improved disease resistance and productivity. This was then followed by studies on abalones using crossbreeding of different geographic populations, H. diversicolor (You et al ) and H. discus hannai (Deng et al ), as well as interspecies hybridization, Haliotis laevigata × Hrubra rubra (Dang et al ; Botwright ), H. discus hannai × Haliotis gigantea (Luo et al ; Liang et al ), Haliotis rufescens × H. discus hannai (Lafarga‐de la et al , ), and H. discus hannai × Haliotis fulgens (You et al ). Genetic breeding of gastropods has also been performed in Physa acuta (Henry et al ), Melanoides tuberculata (Facon et al ), Lymnaea stagnalis (Coutellec and Caquet ), and Cornu aspersum (Nespolo et al ).…”

mentioning

confidence: 99%

Evaluation of Crosses Between Two Geographic Populations of Native Chinese and Introduced Thai Spotted Ivory Shell, Babylonia areolata, in Southern China

Lü

et al. 2016

In the present study, intraspecific crossing experiments between Chinese (H) and Thai populations (T) of Babylonia areolata were conducted by using a complete diallel design (H, T, TH, and HT). A total of 45 pedigreed families were obtained and cultured under the same environmental conditions. Positive heterosis was observed for many developmental parameters and shell sizes for most stages from juvenile to adult. Hatching and metamorphosis occurred significantly earlier in the hybrid (HT) than in the parent groups and a crossed group (TH). Rate of metamorphosis was also higher in HT than in the other three groups. Heterosis in shell length and body weight of the crossed group (HT) from the nursery and grow-out stage to harvest time was high and significantly different from the H, T, and TH groups. For mortality rate from juvenile to adult, total and average heterosis of the crossed group (TH) was positive and significantly different from H, T, and HT. The results of the present study show that intraspecies crosses can be a potentially useful way to increase the economic traits for B. areolata and thus provide a promising method for genetic improvement of the species as a new aquaculture stock in southern China.