Parentage verification in field progeny testing program of Mehsana buffalo1

Abstract

The present study was undertaken to construct a multiplex microsatellite panel for parentage testing in Mehsana buffalo (Bubalus bubalis). The study was based on a total of 212 Mehsana buffalos (100 dams, 100 daughters, and 12 sires). Genomic DNA was extracted from blood and semen samples. A panel of 10 microsatellite markers (CSSM61, ILSTS29, ILSTS17, ILSTS28, CSSM57, CSSM22, ILSTS61, CSSM8, ETH152, and ILSTS11) was amplified in a single multiplex reaction and analyzed by capillary electrophoresis on an automated DNA sequencer. The expected heterozygosity ranged from 0.642 to 0.833 (mean 0.762). The total exclusion probability using 10 microsatellite loci with 1 known parent was 0.993. Seven out of 10 microsatellite loci revealed relatively high polymorphic information content (>0.7). Eighty-one daughters out of 100 daughters qualified by compatibility according to Mendelism. The results suggest that multiplex microsatellite panel is a fast, robust, reliable, and economic tool to verify the parentage as well as to assign the putative sire to daughters under progeny testing with very high accuracy and hence can be used in routine parentage testing.

DNA profiling and paternity verification in Murrah buffaloes

“…This high success could be attributed to the trained manpower of BAIF Pune and also the cooperation of the farmers who were sensitized about the objectives of the project. This value is higher from those obtained from the field (Jakhesara et al 2012) for Mehsana buffaloes as well as in the organized herds abroad (Geldermann et al 1986). In most of the districts belonging to the area of operation of the project paternity was found to be correct while the districts, which were recently adopted by BAIF for AI revealed more number of daughters with incorrect paternity.…”

“…There has been only one report in Mehsana buffaloes of Gujarat where the parentage was tested under field conditions based on 100 dam-daughter pairs and 12 sires (using semen samples of 5 sires for extraction of DNA). A total of 19 daughters out of 100 tested did not match with the recorded sire (Jakhesara et al 2012). In this present study, the recording was at farmers' household at the field level in 52 districts of Uttar Pradesh.…”

“…Declaring the wrong paternity when more than one marker does not match is likely to increase the pedigree error detection rate but would also increase the false positive rate (Jakhesara et al 2012). In the present experiment, most of the wrong paternity was attributable to non amplification of 2 or more markers due to mutation in primer binding sites.…”

Genetic diversity of bovine populations raised in Senegal

“…The nature of the loci involved could be another clue to explain at least part of these deviations. Indeed, 6 of the 7 most involved loci (BM2113, CSSM66, HEL9, INRA063, SPS115 and ETH185) are very sensitive to inbreeding and are used to determine the DNA fingerprints of individuals or conduct paternity‐test (Curi & Lopez, 2002; Jakhesara et al., 2012; Rodríguez‐Ramírez et al., 2011; Rajapaksha et al., 2014; Silva et al., 2014; Brenig and Schütz, 2016). It is therefore possible that part of the heterozygosity deficit observed for certain populations, notably those of the CRZ of Dahra and EMAAP, is due to the inbreeding of individuals that is reflected through these loci.…”

Evaluation of 24 microsatellite markers for parentage exclusion in three indigenous pig types of India

“…Due to their highly polymorphic nature, the use of microsatellite typing for parentage control and solving the problems of questionable paternity had been advocated in almost all livestock species (Behl et al 2007, Souza et al 2012, Monies et al 2011, Jakhesara et al 2012, Kathiravan et al 2012, Brenig and Schulz 2016. Due to multiple mating nature of sows and so the probability of multiple paternity of the litter makes the use of such unambiguous paternity exclusion methodology absolutely necessary for pigs (Aguilera-Reyes 2006, Menéndez et al 2015.…”