Parenteral immunization of fish, Labeo rohita with Poly d, l-lactide-co-glycolic acid (PLGA) encapsulated antigen microparticles promotes innate and adaptive immune responses

Behera, T.; Nanda, Pramod Kumar; Mohanty, Chandana; Mohapatra, Debendra K.; Swain, P.; Das, Basanta Kumar; Routray, P.; Mishra, Birendra Kumar; Sahoo, Sanjeeb K.

doi:10.1016/j.fsi.2009.11.009

Cited by 60 publications

(33 citation statements)

References 37 publications

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“…Se realizó un serial de dilución 1:2 con 25 µl de suero sanguíneo a igual volumen de PBS y se adicionó 25 µl de solución de E. coli (10 8 células/ ml) previamente inactivada con formalina en placa multipozos de fondo en U. Las placas se incubaron en el transcurso de la noche a temperatura ambiente y los títulos se calcularon como el recíproco de la dilución de suero que presentó la más alta aglutinación completa de las células bacterianas (18). process was stopped by cooling on iced sheets as described by Millet et al (15).…”

Section: Colecta De Muestrasunclassified

“…A 1:2 serial dilution was performed with 25 µl of serum to equal volumes of PBS and 25 µl of E. coli solution (108 cells/ml) that was previously inactivated with formalin in a multiwell U bottom plate. The plates were incubated during the night at room temperature and titers were calculated as the reciprocal of the serum dilution that had the highest complete agglutination of the bacterial cells (18).…”

Section: Lysozyme Activity Test For This Test a Bacterial Suspensiomentioning

confidence: 99%

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Supplementation with humic substances affects the innate immunity in layer hens in posfasting phase

2016

Rev MVZ Córdoba

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El suplemento de sustancias húmicas afecta la inmunidad innata en gallinas ponedoras en fase posmuda ABSTRACTObjective. Asses the effect of supplementation with Humic substances (HS) over some innate immunity parameters (serum bactericidal activity, phagocytosis, bacterial agglutination, respiratory burst and lisozyme activity) in phase after fasting of layer hens. Materials and methods. 120 posfasting phase Hy Line Brown layer hens were taken which were distributed into four groups: The first and the second were supplemented with 0.1 and 0.2% of HS, respectively. The third group was supplemented with 0.25 mg/kg on levamisole hydrochloride and fourth group have no supplementation; during sixty days period. Blood samples were collected on 8th, 30th and 60th of experiment day. Results. The phagocytic index and respiratory burst increased significantly at day 30th in HS supplemented groups. Alike, serum bactericidal activity and lisozyme activity improved on 8 th day, nevertheless, changes were no evident latter. The bacterial agglutination was high in supplemented groups evaluated at everyone times. Conclusions. Results showed that HS behave as immunostimulant in the early phase after fasting layer hens.Key words: Agglutination, bactericide activity, Lisozyme, phagocytosis (Source: DeCS). RESUMENObjetivo. Evaluar el efecto de las sustancias húmicas (SH) sobre algunos parámetros de la inmunidad innata (actividad bactericida del suero, fagocitosis, aglutinación bacteriana, explosión respiratoria y actividad de la lisozima) en la fase posmuda de gallinas ponedoras. Materiales y métodos. Se utilizaron 120 gallinas ponedoras Hy Line Brown en la fase de posmuda, las cuales fueron divididas en cuatro grupos: Los dos primeros fueron suplementados con 0.1 y 0.2% de SH respectivamente, el tercer grupo fue suplementado con 0.25 mg/kg de Clorhidrato de levamisol y el cuarto grupo control sin suplemento; durante un período de 60 días. Las muestras sanguíneas se tomaron los días 8, 30 y 60 del experimento. Resultados. El Índice fagocítico y la explosión respiratoria se incrementaron significativamente a partir del día 30 de suplementación con SH. De la misma manera, la actividad bactericida del suero y la actividad de la lisozima aumentaron al día 8; no obstante no se evidenciaron cambios posteriores. La aglutinación bacteriana fue significativamente mayor en los grupos suplementados en todos los tiempos evaluados. Conclusiones. Los resultados demuestran que las SH se comportan como agentes inmunoestimulantes en la fase temprana de la posmuda en gallinas ponedoras Hy Line Brown. Palabras clave: Actividad bactericida, aglutinación, fagocitosis, lisozima (Fuente: DeCS).Rev.MVZ Córdoba 21(1):5198-5210, 2016. ISSN: 0122-0268 ORIGINAL 5199 INTRODUCCIÓNLos mecanismos de la respuesta inmune, innata o adaptativa durante una infección demandan un gasto energético que puede interferir con la capacidad productiva de animales susceptibles. Tradicionalmente, el abordaje de los problemas infecciosos incluye, además, de la disminución de los...

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Section: Colecta De Muestrasunclassified

Section: Lysozyme Activity Test For This Test a Bacterial Suspensiomentioning

confidence: 99%

Supplementation with humic substances affects the innate immunity in layer hens in posfasting phase

2016

Rev MVZ Córdoba

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“…The response is more pronounced in the spleen, where the nanoparticles are reported to accumulate (Fredriksen et al, 2011). Respiratory burst activity (RB), myeloperoxidase activity and other immune parameters were also higher with the use of nanoparticles than with the use of oily adjuvants (Behera et al, 2010), and the response of antibodies was similar to that of oily adjuvants (Behera et al, 2010;Fredriksen & Grip 2012). By 60 and 75 days after administration the nano-and microparticles of gamma globulin from human blood and β-glucan stimulated high levels of antibodies, up to 90 and 120 days, respectively.…”

Section: Poly (Dl-lactic-co-glycolic) Acid (Plga)mentioning

confidence: 96%

“…It has been shown that nano-and microparticles of PLGA encapsulating AG administered by intraperitoneal injection have the adjuvant capacity to stimulate the expression of pro-infl ammatory cytokines and antibodies in a similar manner to that of oily adjuvants in rohu (Labeo rohita) and Atlantic salmon (Behera et al, 2010;Fredriksen & Grip 2012;Fredriksen et al, 2011). The immune response is stimulated in Atlantic salmon within two days after intraperitoneal administration of PLGA nanoparticles of β-glucan.…”

Section: Poly (Dl-lactic-co-glycolic) Acid (Plga)mentioning

confidence: 99%

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Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines

2013

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Aquaculture has become an important economic sector worldwide, but is faced with an ongoing threat from infectious diseases. Vaccination plays a critical role in protecting commercially raised fi sh from bacterial, viral and parasitic diseases. However, the production of eff ective vaccines is limited by the scarcity of knowledge about the immune system of fi sh. Improving vaccines implies using antigens, adjuvants and employing methods of administration that are more eff ective and less harmful to the fi sh. In this context, in recent year there have studies of methods of encapsulating antigens in matrices of diff erent types to apply in fi sh vaccines. This work reviews the new methods to improve fi sh vaccines by encapsulating them in polymers and polysaccharides.

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Adjuvants in Fish Vaccines

Tafalla¹,

Bøgwald

Dalmo

et al. 2014

Fish Vaccination

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Parenteral immunization of fish, Labeo rohita with Poly d, l-lactide-co-glycolic acid (PLGA) encapsulated antigen microparticles promotes innate and adaptive immune responses

Cited by 60 publications

References 37 publications

Supplementation with humic substances affects the innate immunity in layer hens in posfasting phase

Supplementation with humic substances affects the innate immunity in layer hens in posfasting phase

Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines

Adjuvants in Fish Vaccines

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