Three-pronged education" is the core requirement for the current educational work in universities, and grassroots organizations in universities play an important role in this regard. This study aims to explore the educational practice paths of technical branches in grassroots organizations in the "Three-pronged education" system in university colleges. A certain vocational college was chosen as the research object, and methods such as questionnaire surveys and in-depth interviews were used to conduct in-depth research and analysis on the educational practice of technical branches in grassroots organizations in universities. The research results show that the educational practices of technical branches in universities in the "three-pronged education" system mainly include four aspects: firstly, strengthening ideological and political education to improve students' ideological and moral qualities; secondly, organizing participation in professional technical practices to cultivate students' healthy and progressive lifestyles; thirdly, building an excellent academic atmosphere to create a strong learning atmosphere; fourthly, strengthening guidance in professional technical aspects of employment and entrepreneurship to improve students' professional literacy and technical competitiveness. At the same time, this study also puts forward corresponding suggestions for the educational practices of organizations in university departments in the "three-pronged education" system, including strengthening the construction of organizations themselves and improving the level of educational practices; innovating educational methods and expanding educational channels; strengthening the evaluation of educational effectiveness and continuously improving educational work, etc.