Parity among interpretation methods of MLEE patterns and disparity among clustering methods in epidemiological typing of Candida albicans

Boriollo, Marcelo Fabiano Gomes; Rosa, Edvaldo Antônio Ribeiro; Gonçalves, Reginaldo Bruno; Höfling, José Francisco

doi:10.1016/j.mimet.2005.05.012

Cited by 20 publications

(64 citation statements)

References 55 publications

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“…; Boriollo et al . ). It outperforms many of the DNA‐based methods and remains a useful tool for molecular investigation of natural populations of C. albicans (Santos et al .…”

Section: Non‐dna‐based Typing Methodsmentioning

confidence: 97%

See 1 more Smart Citation

Molecular methods for strain typing of Candida albicans : a review

Saghrouni¹,

Abdeljelil²,

Boukadida³

et al. 2013

J Appl Microbiol

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SummaryCandida albicans is one of the most medically important fungi because of its high frequency as a commensal and pathogenic microorganism causing superficial as well as invasive infections. Strain typing and delineation of the species are essential for understanding its biology, epidemiology and population structure. A wide range of molecular techniques have been used for this purpose including non-DNA-based methods (multi-locus enzyme electrophoresis), conventional DNA-based methods (electrophoretic karyotyping, random amplified polymorphic DNA, amplified fragment length polymorphism, restriction enzyme analysis with and without hybridization, rep-PCR) and DNA-based methods called exact typing methods because they generate unambiguous and highly reproducible typing data (including microsatellite length polymorphism and multi-locus sequence typing). In this review, the main molecular methods used for C. albicans strain typing are summarized, and their advantages and limitations are discussed with regard to their discriminatory power, reproducibility, cost and ease of performance.

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“…; Boriollo et al . ). It outperforms many of the DNA‐based methods and remains a useful tool for molecular investigation of natural populations of C. albicans (Santos et al .…”

Section: Non‐dna‐based Typing Methodsmentioning

confidence: 97%

“…Finally, MLEE is time‐consuming because it requires the combination of results from at least 10 enzymes to provide reliable typing data and detection of variability among isolates (Boriollo et al . , ).…”

Section: Non‐dna‐based Typing Methodsmentioning

confidence: 99%

Molecular methods for strain typing of Candida albicans : a review

Saghrouni¹,

Abdeljelil²,

Boukadida³

et al. 2013

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…Many techniques are used for molecular characterization of Candida species, such as Restriction Fragment Length Polymorphism (RFLP) [41], DNA fingerprinting [42], electrophoretic karyotyping [43], Random Amplified Polymorphic DNA (RAPD) [44], Multilocus Enzyme Electrophoresis (MLEE) [45], and sequencing of microsatellites [46]. However, in order to estimate genetic distances and infer phylogenetic relationships between species that can be easily evaluated in terms of probability models, the most suitable techniques involve gene sequencing and produce topologies built from nucleotide or amino acid sequences.…”

Section: Molecular Methods Developed For the Identification And Charamentioning

confidence: 99%

Molecular Methods Developed for the Identification and Characterization of Candida Species

Grisolia¹

2017

IJGS

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Candida species are commensally microorganisms in healthy individuals, but is also the most opportunistic fungal pathogen of human. From benign skin-mucosal forms to invasive ones, Candida infection, can compromise various organs systematic and cause diseases in immunocompromised or critically ill patients. Hence the accurate identification and characterization of the disease-causing strains is crucial for diagnosis, clinical treatment and epidemiological studies of candidacies. Molecular methods the identification and characterization support gives in epidemiological research and in development of novel antifungal with specific molecular targets, especially in the case of resistant Candida species. This review aims to describe the main methods used in the identification and characterization the Candida species and discusses future perspectives.

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“…Therefore, if the enzyme banding patterns of two isolates differ, such differences are assumed to be DNA based and heritable. MLEE is a reliable technique with a relatively high discriminatory power in the distinction between unrelated strains and shows good reproducibility [30], [31].In the field of medical mycology, isoenzyme typing has shown great potential in studies of taxonomic, systematics, genetics, evolutive, and epidemiologic characterization, especially for the yeast C. albicans [32]. An enzyme reaction can be demonstrated by electrophoretic bands of enzymatic activity in gels, indicating the existence of isoenzymes or isozymes.…”

Section: Methods For Identification Of Candida Speciesmentioning

confidence: 99%

Molecular-Genetic Approaches for Identification and Typing of Pathogenic Candida Yeasts: A Review

Singh¹

2014

IJIRSET

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Candida is a medically important fungi because of its high frequency as a commensal and pathogenic microorganism causing superficial as well as invasive infections. Because the accurate diagnosis of candidiasis remains difficult, a fast and reliable assay for characterization of fungal pathogens is critical for the early initiation of adequate antifungal therapy and/or for introduction of preventive measures. As novel molecular genetic techniques are continuously introduced, their role in the management of infectious diseases has also been growing .Strain typing and delineation of the species are essential for understanding its biology, epidemiology and population structure. A wide range of molecular techniques have been used for this purpose including non-DNA-based methods (multi-locus enzyme electrophoresis), conventional DNA-based methods (electrophoretic karyotyping, random amplified polymorphic DNA, amplified fragment length polymorphism, restriction enzyme analysis with and without hybridization, rep-PCR) and DNA-based methods called exact typing (PCR based methods, microstallite length polymorphism, multilocus sequence typing, DNA microarray ) because they generate unambiguous and highly reproducible typing data. Today, molecular strategies complement conventional methods and provide more accurate and detailed insight. It can be expected that future technical development will improve their potential furthermore.In this article, we provide a critical review on the vigorously fermenting field of molecular approaches to Candida identification and typing and summarized their advantages and limitations with regard to their discriminatory power, reproducibility, cost and ease of performance.

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Parity among interpretation methods of MLEE patterns and disparity among clustering methods in epidemiological typing of Candida albicans

Cited by 20 publications

References 55 publications

Molecular methods for strain typing of Candida albicans : a review

Molecular methods for strain typing of Candida albicans : a review

Molecular Methods Developed for the Identification and Characterization of Candida Species

Molecular-Genetic Approaches for Identification and Typing of Pathogenic Candida Yeasts: A Review

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