Parmotrema screminiae (Parmeliaceae), a Novel Lichen Species from Brazil with Potent Antimicrobial Activity

Honda, Nobuaki; Freitas, Deisy Santos; Micheletti, Ana Camila; Carvalho, Nádia Cristina Pereira; Spielmann, Adriano Afonso; Canêz, Luciana da Silva

doi:10.17807/orbital.v8i6.877

Cited by 5 publications

(10 citation statements)

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“…utilizados neste estudo. Amostras clínicas de S. aureus multirresistentes já foram citadas em outros trabalhos como sensíveis a extratos de Parmotrema coletados em outras localidades 22,23 .…”

Section: Resultsunclassified

“…Esses testes também permitiram identificar o ácido girofórico no extrato acetônico de Parmotrema sp. Várias substâncias já foram citadas para o gênero, como é o caso de atranorina, norlobaridona e ácido úsnico23,28 ; porém, esta é a primeira referência do ácido girofórico para espécies do gênero Parmotrema, o qual não apresentou ação antimicrobiana, como observado na bioautografia. Apesar dos relatos sobre a ação antimicrobiana do ácido girofórico18,29 , é provável que sua concentração, no extrato acetônico da amostra de Parmotrema sp.…”

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Atividade antimicrobiana de fungos liquenizados ocorrentes no campus Belém da Universidade Federal do Pará, estado do Pará, Brasil

Vieira

Pinheiro

et al. 2019

Revista Pan-Amazônica de Saúde

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Avaliar a atividade antimicrobiana de extratos obtidos a partir de fungos liquenizados ocorrentes no campus Belém da Universidade Federal do Pará, Brasil. MATERIAIS E MÉTODOS: Amostras liquênicas de Leptogium sp. e Parmotrema sp. foram submetidas à extração orgânica por esgotamento a frio, seguindo a série eluotrópica clorofórmio e acetona. Os extratos obtidos foram testados frente a cepas de bactérias Gram-positivas, Gram-negativas e álcool-ácido resistentes, fungos filamentosos e leveduras, empregando-se o método de disco difusão em ágar. Discos de papel foram impregnados com 20 µL de cada extrato na concentração de 4 mg/mL e depositados sobre o meio previamente inoculado com os microrganismos teste. Os resultados foram avaliados pelo diâmetro dos halos de inibição em torno dos discos. Em seguida, os extratos foram submetidos à cromatografia ascendente em camada delgada e a ensaios bioautográficos para detecção dos princípios ativos. RESULTADOS: Houve maior potencial antimicrobiano dos extratos de Parmotrema sp. com halos de inibição que variaram de 8 a 12 mm para bactérias e de 8 a 21 mm para fungos. As bactérias Escherichia coli, Klebsiella pneumoniae e Mycobacterium phlei apresentaram maior sensibilidade, pois foram inibidas pelas duas espécies liquênicas. Em relação aos fungos, os mais sensíveis foram Trichophyton tonsurans, Microsporum gypseum e Epidermophyton floccosum. CONCLUSÃO: Este trabalho ampliou o conhecimento sobre fungos liquenizados da Região Amazônica e possibilitou a obtenção de substâncias antimicrobianas viáveis para a inibição do crescimento de bactérias e fungos de importância médica.

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Section: Resultsunclassified

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Atividade antimicrobiana de fungos liquenizados ocorrentes no campus Belém da Universidade Federal do Pará, estado do Pará, Brasil

Vieira

Pinheiro

et al. 2019

Revista Pan-Amazônica de Saúde

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“…For example, norlobaridone acid from lichens was found to inh Phosphoprotein 1 in a study by Talapatra et al [17], and showed potent antibiotic activity against S. aureus and E. faecalis according to Honda at al. [16]; While as, atranorin showed neurotrophic activity in Neuro2A cells [18]. In previous study by Kosanić et al antioxidant, antimicrobial and cytotoxic activities of atranorin was extracted from several natural lichens [19].…”

Section: Hplc Analysis and Antioxidant Capacity (Dpph Radical Scavenging) Of Parmelia Lichen Extractsmentioning

confidence: 97%

“…HPLC analysis results revealed that the predominant lichen acids in acetone extract were norlobaridone and atranorin acid, whereas, in methanol extract was usnic acid. According to the study of Honda at al. (2016), showing that acetone extract of Parmotrema screminiae is a remarkable source of norlobaridone and atranorin acid [16].…”

Section: Hplc Analysis and Antioxidant Capacity (Dpph Radical Scavenging) Of Parmelia Lichen Extractsmentioning

confidence: 99%

“…According to the study of Honda at al. (2016), showing that acetone extract of Parmotrema screminiae is a remarkable source of norlobaridone and atranorin acid [16]. In addition, depsidones/depsides from acetone extract (norlobaridone and atranorin acid) have been mentioned in several previous studies for their biological functions.…”

Section: Hplc Analysis and Antioxidant Capacity (Dpph Radical Scavenging) Of Parmelia Lichen Extractsmentioning

confidence: 99%

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Inhibitory Activity of Extracts Isolated from Parmelia Lichen against Influenza A Virus (H1N1)

Cuong

Kim

2018

AJOB

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Aims: In the present study, we aim to investigate the antiviral effect against influenza A (H1N1) virus of extracts of Parmelia lichen using the MDCK cell line. Methodology: Parmelia lichens species were collected and extracted using acetone and methanol solvents, then chemically analyzed by HPLC. The antioxidant of the lichen extracts was measured using the DPPH method, meanwhile, cytotoxicity and antiviral activity were tested using the CCK-8 method. The cytopathic effect and apoptosis of infected-cells were observed under microscopy and using Muse Annexin V and Dead Cell Kit stains in the Muse Cell Analyzer. Finally, to confirm the antiviral effects of lichen against influenza A virus, the relative expression level of viral mRNA PAgene in MDCK cells was measured using the qRT-PCR method. Results: Based on the in vitro assays, we found that methanolic extract of Parmelia lichen shows extremely high activity against IAV, which is a significantly higher cell viability (more than 40%); however, a relatively low apoptosis and cell death compared to positive IAV-infected cells. Furthermore, the methanolic lichen extracts showed a significant reduction (by 1.9 log-fold) of influenza A viral replication with concentration of 100 μg/mL. Conclusion:Our results show that methanolic extracts of natural Parmelia lichen highly inhibited influenza A virus replication in vitro assays. This knowledge has, in turn, allowed the researchers to further explore the specific molecules from natural lichens for pharmaceutical biomaterials of a new anti-influenza therapy.

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Antibacterial potencial of 12 Lichen species

Micheletti

Honda

Ravaglia

et al. 2021

An. Acad. Bras. Ciênc.

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Resistant bacterial infections are a major public health problem worldwide, which entails the need to search for new therapeutic agents. In this context, lichens stand out, provided that they are producers of structurally diverse compounds that have attractive biological properties, including antimicrobial activity. Thus, extracts of 12 lichen species were prepared and their potential to inhibit the growth of 5 bacterial strains was evaluated in this work. The chemical compositions of these extracts were examined using TLC and microcrystallization, being the identity of the active compounds in each extract attributed based on the bioautography technique. The most active extracts (and their identifi ed active compounds) were from Cladonia borealis (usnic, barbatic and 4-O-demethylbarbatic acids), Cladina confusa (usnic and perlatolic acids), Stereocaulom ramulosum (atranorin, perlatolic and anziaic acids) and Canoparmelia cryptochlorophaea (cryptochlorophaeic and caperatic acids), with MICs ranging from 7.8 to 31.25 μg/mL, including for resistant clinical strains. MIC values were also obtained for substances isolated from lichens for comparison purposes. A group of four extracts containing usnic acid was analyzed by 1 H NMR in order to correlate relative proportion of major metabolites and extracts activity. The less active extracts in this group, in fact, presented low proportion of usnic acid.

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Parmotrema screminiae (Parmeliaceae), a Novel Lichen Species from Brazil with Potent Antimicrobial Activity

Cited by 5 publications

References 1 publication

Atividade antimicrobiana de fungos liquenizados ocorrentes no campus Belém da Universidade Federal do Pará, estado do Pará, Brasil

Atividade antimicrobiana de fungos liquenizados ocorrentes no campus Belém da Universidade Federal do Pará, estado do Pará, Brasil

Inhibitory Activity of Extracts Isolated from Parmelia Lichen against Influenza A Virus (H1N1)

Antibacterial potencial of 12 Lichen species

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