PARP‐1 modulates the expression of miR‐223 through histone acetylation to involve in the hydroquinone‐induced carcinogenesis of TK6 cells

Abstract

The upstream regulators of microRNAs were rarely reported. Hydroquinone (HQ) is the main metabolite of benzene, one of the important environmental factors contributing to leukemia and lymphoma. In HQ-induced malignant transformed TK6 (TK6-HT) cells, the expression of PARP-1 and miR-223 were upregulated. When in PARP-1 silencing TK6-HT cells, miR-223 was downregulated and the apoptotic cell number correspondingly increased. In TK6 cells treated with HQ for different terms, the expression of miR-223 and PARP-1 were dynamically observed and found to be decreased and increased, respectively. Trichostatin A could increase the expression of miR-223, then the expression of HDAC1-2 and nuclear factor kappa B were found to be increased, but that of mH2A was decreased. PARP-1 silencing inhibited the protein expression of H3Ac, mH2A, and H3K27ac. By co-immunoprecipitation experiment, PARP-1 and HDAC2 were found to form a regulatory complex. In conclusion, we demonstrated that the upregulation of PARP-1 mediated activation of acetylation to promote the transcription of miR-223 possibly via coregulating with HDAC2, an epigenetic regulation mechanism involved in cell malignant transformation resulting from long-term exposure to HQ, in which course, H3K27ac might be a specific marker for the activation of histone H3, which also gives hints for benzene exposure research.

DNMT1 regulates hypermethylation and silences hsa_circ_401351 in hydroquinone‐induced malignant TK6 cells

“…35,36 Furthermore, we previously discovered that HQ-TK6 cells have significant levels of DNA methylation, which could be effective for controlling the expression of lncRNAs and miRNAs. 20,24,37,38 Consequently, we employed 5-AzaC and TSA to treat HQ-TK6 cells to investigate whether DNA hypermethylation is involved in regulating and changing circRNA expression. The expression of hsa_-circ_401351, which had been reduced previously, was rescued after HQ-TK6 cells were treated with 5-AzaC and TSA.…”

“…DNA methylation is a widely accepted epigenetic modification that regulates gene expression at the transcriptional level 35,36 . Furthermore, we previously discovered that HQ‐TK6 cells have significant levels of DNA methylation, which could be effective for controlling the expression of lncRNAs and miRNAs 20,24,37,38 . Consequently, we employed 5‐AzaC and TSA to treat HQ‐TK6 cells to investigate whether DNA hypermethylation is involved in regulating and changing circRNA expression.…”

Skin Anti-Aging Potential through Whitening and Wrinkle Improvement Using Fermented Oil Derived from Hermetia illucens Larvae

“…Additionally, concerns persist regarding the adverse effects of the synthetic ingredients used in anti-aging cosmetics. An example of these is hydroquinone, which is a key ingredient in whitening cosmetics and has been classified as a carcinogen [ 10 ]. As a result, there is growing interest in the research and development of highly functional natural cosmetic ingredients that are both effective and safe, particularly those related to the prevention of skin aging, such as through whitening and wrinkle improvement [ 11 ].…”