Parsing the microRNA genetics basis regulating skeletal muscle fiber types and meat quality traits in pigs

Jiang, Aiwen; Yin, Di; Zhang, L; Li, Bisi; Li, R; Zhang, X; Zhang, Z; Kim, K; Wu, Wangjun

doi:10.1111/age.13064

Cited by 12 publications

(11 citation statements)

References 43 publications

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“…First, according to the observed muscle fiber characteristics, we selected PM and BF as representatives of type I muscle fibers (oxidative type) and type II muscle fibers (glycolytic type), respectively. Similar to previous reports, PM was characterized as the slow oxidative fiber type (type I), as found in pork [ 27 , 28 ], beef [ 29 ] and goat muscles [ 30 ], whereas BF showed higher respective percentages of type II fibers (fast-twitch fibers) [ 4 , 13 , 31 ]. Generally, the diameter of type I muscle fibers is thinner than that of type II muscle fibers [ 32 ], and our data were consistent with this pattern.…”

Section: Discussionsupporting

confidence: 86%

“…MicroRNAs can regulate target gene expression by promoting mRNA degradation or repressing translation. Several studies have also identified some differentially expressed miRNAs between glycolytic and oxidative muscles [ 13 , 15 , 16 , 17 ]. At the miRNA level, the present study is the first to identify a total of 21 known DEmiRs in comparisons of PM (mainly composed of slow-twitch muscle fibers) and BF (mainly composed of fast-twitch muscle fibers).…”

Section: Discussionmentioning

confidence: 99%

“…MicroRNAs (miRNAs), which are important regulators of gene expression at the post-transcriptional level, have also been reported to play an important role in skeletal muscle growth and development [ 10 , 11 ]. Moreover, studies have broadened our understanding of the role of some muscle-specific miRNAs in the regulation of muscle fiber type composition and specification by regulating the expression of muscle-fiber-type-specific genes and associated transcription factors in certain livestock [ 12 , 13 , 14 , 15 ]. For example, Wang et al showed that miR-499-5p acts as a positive regulator of oxidative myofiber formation in pigs by silencing Sox6 mRNA expression and increasing MyHC I and MyHC IIa mRNA levels [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

Shi

et al. 2022

Genes

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The skeletal muscle fiber profile is closely related to livestock meat quality. However, the molecular mechanisms determining muscle fiber types in donkeys are not completely understood. In this study, we selected the psoas major muscle (PM; mainly composed of oxidative-type muscle fibers) and biceps femoris muscle (BF; mainly composed of glycolytic-type muscle fibers) and systematically compared their mRNA and microRNA transcriptomes via RNA-seq. We identified a total of 2881 differentially expressed genes (DEGs) and 21 known differentially expressed miRNAs (DEmiRs). Furthermore, functional enrichment analysis showed that the DEGs were mainly involved in energy metabolism and actin cytoskeleton regulation. The glycolysis/gluconeogenesis pathway (including up-regulated genes such as PKM, LDHA, PGK1 and ALDOA) was more highly enriched in BF, whereas the oxidative phosphorylation pathway and cardiac muscle contraction (including down-regulated genes such as LDHB, ATP2A2, myosin-7 (MYH7), TNNC1, TPM3 and TNNI1) was more enriched in PM. Additionally, we identified several candidate miRNA–mRNA pairs that might regulate muscle fiber types using the integrated miRNA–mRNA analysis. Combined with the results of protein–protein interaction (PPI) analysis, some interesting DEGs (including ACTN3, TNNT3, TPM2, TNNC2, PKM, TNNC1 and TNNI1) might be potential candidate target genes involved in the miRNA-mediated regulation of the myofibril composition. This study is the first to indicate that DEmiRs, especially eca-miR-193a-5p and eca-miR-370, and potential candidate target genes that are mainly involved in actin binding (e.g., ACTN3, TNNT3 and TNNC1) and the glycolysis/gluconeogenesis pathways (e.g., PKM) might coregulate the myofibril composition in donkeys. This study may provide useful information for improving meat quality traits in Dezhou donkeys.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

Shi

et al. 2022

Genes

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“…Recent study has found that miR-499-3p could suppress retinal cell proliferation while promote apoptosis to induce diabetic retinopathy by enhancing activation of the TLR4 signaling pathway [ 15 ]. In pigs, the expression of ssc-miR-499-3p was significantly correlated to the expression of myoglobin and pH, prompting its potential regulatory role in skeletal muscle fiber transformation and meat quality traits [ 16 ]. However, the exact biological function of miR-499-3p in skeletal muscle development has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis

Cai

Zhou

et al. 2022

J Animal Sci Biotechnol

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Background Circular RNAs (circRNAs) are a novel class of endogenous ncRNA, which widely exist in the transcriptomes of different species and tissues. Recent studies indicate important roles for circRNAs in the regulation of gene expression by acting as competing endogenous RNAs (ceRNAs). However, the specific role of circRNAs in myogenesis is still poorly understood. In this study, we attempted to systematically identify the circRNAs involved in myogenesis and analyze the biological functions of circRNAs in chicken skeletal muscle development. Results In total, 532 circRNAs were identified as being differentially expressed between pectoralis major (PEM) and soleus (SOL) in 7-week-old Xinghua chicken. Among them, a novel circRNA (novel_circ_002621), generated by PTPN4 gene, was named circPTPN4 and identified. circPTPN4 is highly expressed in skeletal muscle, and its expression level is upregulated during myoblast differentiation. circPTPN4 facilitates the proliferation and differentiation of myoblast. Moreover, circPTPN4 suppresses mitochondria biogenesis and activates fast-twitch muscle phenotype. Mechanistically, circPTPN4 can function as a ceRNA to regulate NAMPT expression by sponging miR-499-3p, thus participating in AMPK signaling. Conclusions circPTPN4 functions as a ceRNA to regulate NAMPT expression by sponging miR-499-3p, thus promoting the proliferation and differentiation of myoblast, as well as activating fast-twitch muscle phenotype.

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“…Genetic mutations in miR-208b and miR-1 precursor genes are significantly associated with pig muscle fiber characteristics and meat color traits ( 8 , 9 ). The expression of miR-499 is significantly correlated with the expression of myoglobin , which typically reflects meat color ( 10 ). However, few studies have explored the mechanism of miRNA involvement in the regulation of pig meat color traits.…”

Section: Introductionmentioning

confidence: 99%

A polymorphism in porcine miR-22 is associated with pork color

et al. 2022

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MicroRNAs (miRNAs) are posttranscriptional regulators that play key roles in meat color regulation. Changes in miRNA expression affect their target mRNAs, leading to multifunctional effects on biological processes and phenotypes. In this study, a G > A mutation site located upstream of the precursor miR-22 sequence in Suhuai pigs was significantly correlated with the meat color parameter a*(redness) of the porcine longissimus dorsi (LD) muscle. AA genotype individuals had the highest average meat color a* value and the lowest miR-22 level. When G > A mutation was performed in the miR-22 overexpression vector, miR-22 expression significantly decreased. Considering that Ca2+ homeostasis is closely related to pig meat color, our results further demonstrated that ELOVL6 is a direct target of miR-22 in pigs. The effects of miR-22 on skeletal muscle intracellular Ca2+ were partially caused by the suppression of ELOVL6 expression.

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Parsing the microRNA genetics basis regulating skeletal muscle fiber types and meat quality traits in pigs

Cited by 12 publications

References 43 publications

Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis

A polymorphism in porcine miR-22 is associated with pork color

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