Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy

Abstract

Please cite this article as: Alinda R. Fernandes, Divya M. Chari, Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy, Journal of Controlled Release (2016Release ( ), doi: 10.1016Release ( /j.jconrel.2016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT

A fusion of minicircle DNA and nanoparticle delivery technologies facilitates therapeutic genetic engineering of autologous canine olfactory mucosal cells

“…900 nm (polydispersity index of 0. 24) with the addition of the mC construct encoding BDNF. 25 The zeta potential of the resulting complex was shown to be 5.5 mV.…”

“…Further, the approach can be flexibly combined with advanced DNA minicircle (mC) vectors to safely deliver reporter/therapeutic genes to NSCs with similar efficiencies to viral vectors. 24,25 Despite the translational promise of this approach, application of this fusion of novel technologies to clinical cell populations has never been tested.…”

Flexible Ferrofluids: Design and Applications

“…The fifth is improved treatment effects and enlarged varieties of diseases. Copious types of chronic and fatal diseases, such as regenerative neuropathy, autoimmune disease, cardiovascular disease, and cancer, have been demonstrated to be cured or relieved …”

Magnetic Nanoparticle‐Mediated Gene Delivery to Two‐ and Three‐Dimensional Neural Stem Cell Cultures: Magnet‐Assisted Transfection and Multifection Approaches to Enhance Outcomes

“…Magneto-multifection in monolayers has been shown to increase transfection efficiency from ß43% to 52% with a concomitant doubling in mean cell fluorescence intensity when introducing green fluorescent protein (GFP) encoded by minicircles (plasmid DNA without the bacterial backbone), suggesting higher levels of expression compared to single transfections (Fernandes & Chari, 2016a).…”

“…Strong evidence suggests that the use of mini-circle vector systems may be beneficial in this regard. These vectors are further suited to translation as they contain no bacterial sequence elements (e.g., antibiotic resistance genes and origin of replication)meaning they are safer than conventional plasmids and also demonstrate sustained gene expression due to reduction of mammalian transgene silencing mechanisms (Fernandes & Chari, 2016a). Minicircle DNA encoding brain-derived neurotrophic factor (BDNF) has been successfully delivered to NSCs using magnetofection technology, with functional in vitro effects observed from secreted BDNF, including enhanced NSC proliferation and differentiation into neurons (Fernandes & Chari, 2016b).…”