Parthenocarpy restores fruitfulness in sterile triploid (3x) tomatoes artificially obtained by crossing 4x x 2x somaclones

Habashy, A. A.; Testa, Giuseppe; Mosconi, Pietro; Caccia, R.; Mazzucato, Andrea; Santangelo, Enrico; Soressi, G. P.

doi:10.1080/14620316.2004.11511768

Cited by 7 publications

(5 citation statements)

References 23 publications

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“…Altogether, the data collected suggest that SlHB15 is an inhibitor of IAA signaling (Clepet et al, 2021;da Silva and Nogueira, 2021), in agreement with phenotypes of the pat mutant that involve increased IAA response, such as parthenocarpy, increased root development and higher regenerative capacity (Habashy et al, 2004). Also, the feminization of stamens occurring in pat (Mazzucato et al, 1998) is in line with an IAA response (Sawhney and Shukla, 1994).…”

Section: The Hd Zip III Effect On Fruit Set Is Mediated By Auxinsupporting

confidence: 76%

“…The hoc T-DNA mutant has one of the most drastic phenotypes, including the ability to regenerate whole plants in vitro without added phytohormones (Catterou et al, 2002). Similarly, pat showed a higher regeneration index than the WT (Habashy et al, 2004), also in parallel with Aux/IAA9 parthenocarpic knockouts (Wang et al, 2005).…”

Section: The Pat Mutant Shares Vegetative and Reproductive Phenotypes...mentioning

confidence: 99%

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Genetic and molecular mechanisms underlying the parthenocarpic fruit mutation in tomato

Picarella,

Ruiu,

Selleri

et al. 2024

Front. Plant Sci.

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Parthenocarpy allows fruit set independently of fertilization. In parthenocarpic-prone tomato genotypes, fruit set can be achieved under pollen-limiting environmental conditions and in sterile mutants. Parthenocarpy is also regarded as a quality-related trait, when seedlessness is associated with positive fruit quality aspects. Among the different sources of genetic parthenocarpy described in tomato, the parthenocarpic fruit (pat) mutation is of particular interest because of its strong expressivity, high fruit set, and enhanced fruit quality. The complexity of the pat “syndrome” associates a strong competence for parthenocarpy with a complex floral phenotype involving stamen and ovule developmental aberrations. To understand the genetic basis of the phenotype, we mapped the pat locus within a 0.19-cM window of Chr3, comprising nine coding loci. A non-tolerated missense mutation found in the 14th exon of Solyc03g120910, the tomato ortholog of the Arabidopsis HD-Zip III transcription factor HB15 (SlHB15), cosegregated with the pat phenotype. The role of SlHB15 in tomato reproductive development was supported by its expression in developing ovules. The link between pat and SlHB15 was validated by complementation and knock out experiments by co-suppression and CRISPR/Cas9 approaches. Comparing the phenotypes of pat and those of Arabidopsis HB15 mutants, we argued that the gene plays similar functions in species with fleshy and dry fruits, supporting a conserved mechanism of fruit set regulation in plants.

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Section: The Hd Zip III Effect On Fruit Set Is Mediated By Auxinsupporting

confidence: 76%

Section: The Pat Mutant Shares Vegetative and Reproductive Phenotypes...mentioning

confidence: 99%

Genetic and molecular mechanisms underlying the parthenocarpic fruit mutation in tomato

Picarella,

Ruiu,

Selleri

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…It is mostly formed by the fusion product of three haploid nuclei, one from the male gametophyte and two from the female gametophyte and is, therefore, triploid (Thomas et al 2000). Among polyploid cultivars, triploidy, a genomic condition that is favorable for vigor and vegetative productivity, has proved the best for agricultural use (Habashy et al 2004). Triploid plants are usually seed sterile and is undesirable where seeds are of commercial value.…”

Section: Introductionmentioning

confidence: 99%

Production of triploid plants of papaya by endosperm culture

Sun

Liang

et al. 2010

Plant Cell Tiss Organ Cult

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Triploid papaya (Carica papaya) plants were obtained by immature endosperm culture. Visible callusing of the endosperm occurred 21 days after initiation of cultures. A continuously growing callus was observed and a maximum of 68.7% of callus induction frequency was obtained when immature endosperm with embryo was cultured on Murashige and Skoog (MS) medium containing 6.0 lM 2,4-dichlorophenoxyacetic acid (2,4-D), 2.5 lM a-naphthaleneacetic acid (NAA) and 4.0 lM 6-furfurylamino purine (Kn). Shoot buds were produced when the callus was subcultured on a medium supplemented with 6-benzyladenine (BA) or thidiazuron (TDZ) along with NAA. Shoots were detached from the callus and transferred to the elongation medium supplemented with indole-3-acetic acid (IAA) and BA. The combination of 3.0 lM IAA and 1.5 lM BA was the best in terms of the number of cultures (93.8%) showing axillary shoot proliferation. The addition of 2.0 lM indole-3-butyric acid (IBA) to the 1/2 MS medium was most effective at inducing root formation with 90% of the shoots developing four to five roots. Healthy rooted plantlets were transferred to pots containing sterilized bed soil and perlite (3:1) mixture in the greenhouse and 78% of the micropropagated plants survived transplantation. The leaves from endosperm-derived plants showed larger stomata and more chloroplasts in guard cells than that from the parent plants. Over 75% of the endosperm-derived plants were triploid with chromosome number 2n = 3x = 27.

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“…Teknik genom editing antara TALENs, ZFNs, dan CRISPR atau Cas9 telah dikembangkan lebih mengarah pada pengembangan teknologi berbasis sequence nuclease secara spesifik, termasuk Zinc Finger Nucleases atau ZFNs (Kim et. al., 1996) Chen et al, 2003;Habashy et al, 2004;Mackiewicz et al, 1998 Mentimun Kultivar…”

Section: Partenokarpi Buatanunclassified

Pemanfaatan fenomena pembentukan buah partenokarpi dalam perspektif pertanian di Indonesia

Rezaldi

Qonit²,

Nuraini³

et al. 2019

kultivasi

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Fruit is a reproductive organ that plays an important role in plants for plant propagation and seed dispersal. Seeds will be formed due to the process of pollination and fertilization. In some types of fruit, seed in the fruit is not expected, therfore recently many study have been conducted to develop seedless fruit (parthenocarpic fruit). Seedless fruit consists of two main groups namely natural and artificial partenocarpic.Bananas, tomatoes, and mangosteen are examples of natural parthenocarpy plants. Artificial parthenokarpi fruit can be developed in several methods including the use of plant hormone i.e. auksin or gibberellin, pollen iridiation, changes in chromosome number, gene silencing, gene modification, and genome editing tools. In this review, it is explained about the formation of parthenocarp fruit through a biotechnology approach.

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Parthenocarpy restores fruitfulness in sterile triploid (3x) tomatoes artificially obtained by crossing 4x x 2x somaclones

Cited by 7 publications

References 23 publications

Genetic and molecular mechanisms underlying the parthenocarpic fruit mutation in tomato

Genetic and molecular mechanisms underlying the parthenocarpic fruit mutation in tomato

Production of triploid plants of papaya by endosperm culture

Pemanfaatan fenomena pembentukan buah partenokarpi dalam perspektif pertanian di Indonesia

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