Parthenolide accumulation and expression of genes related to parthenolide biosynthesis affected by exogenous application of methyl jasmonate and salicylic acid in Tanacetum parthenium

Majdi, Mohammad; Abdollahi, Mohammad Reza; Maroufi, Asad

doi:10.1007/s00299-015-1837-2

Cited by 37 publications

(22 citation statements)

References 46 publications

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“…4B) and the expression of all four genes were closely related to rutin biosynthesis. In agreement with these results, MeJA has been identified as a predominant inducer of global genes involved in secondary metabolite pathways in various plants, such as Ocimum basilicum 36 , Tanacetum parthenium 18 and S. khuzistanica Jamzad 35 . All the four genes related to rutin biosynthesis investigated in this study, were upregulated by MeJA and SA treatments.…”

Section: Discussionsupporting

confidence: 75%

“…This indicates that probably MeJA has stronger effect on rutin biosynthesis compared with SA. It has been also reported that MeJA was a better elicitor than SA for the production of rosmarinic acid in C. forskohlii hairy root cultures 13 , and also parthenoide production in T. parthenium leaves 18 . As described in a previous report 39 .…”

Section: Discussionmentioning

confidence: 90%

“…Besides this, SA as a signal molecule is involved in many physiological and biochemical processes within plants 16 . SA and its analogues are used comparatively lesser than MeJA for elicitation studies 17,18 . Like MeJA, SA also induces many plant secondary metabolites such as isoflavonoids, terpenes, phytosterols and phenylpropanoids 19 and plays an important role in plant response to a variety of environmental stresses [20][21][22] .…”

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confidence: 99%

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Identification and tissue-specific expression of rutin biosynthetic pathway genes in Capparis spinosa elicited with salicylic acid and methyl jasmonate

Kianersi

Abdollahi

Mirzaie-Asl

et al. 2020

Sci Rep

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Capparis spinosa is an edible medicinal plant which is considered as an excellent source of rutin. Rutin is a glycoside of the flavonoid quercetin that has been reported to have a beneficial role in controlling various diseases such as hypertension, arteriosclerosis, diabetes, and obesity. In this study, the partial cDNA of four genes involved in the rutin biosynthetic pathway including 4-coumaroyl CoA ligase (4CL), flavonoid 3′-hydroxylase (F3′H), flavonol synthase (FLS) and flavonol-3-O-glucoside L-rhamnosyltransferase (RT) were identified in C.spinosa plants for the first time. The protein sequences of these genes shared high similarity with the same proteins in other plant species. Subsequently, the expression patterns of these genes as well as rutin accumulation in C.spinosa leaves treated with different concentrations of salicylic acid (SA) and methyl jasmonate (MeJA) and also in different tissues of Caper plants treated with 100 mgL −1 SA and 150 μM MeJA were evaluated. The expression of all four genes was clearly up-regulated and rutin contents increased in response to MeJA and SA treatments after 24 h. The highest rutin contents (5.30 mgg −1 DW and 13.27 mgg −1 DW), as well as the highest expression levels of all four genes, were obtained using 100 mgL −1 SA and 150 μM MeJA, respectively. Among the different tissues, the highest rutin content was observed in young leaves treated with 150 μM MeJA, which corresponded to the expression of related genes, especially RT, as a key gene in the rutin biosynthetic pathway. These results suggest that rutin content in various tissues of C. spinosa can be enhanced to a significant extent by MeJA and SA treatments and the gene expression patterns of rutin-biosynthesis-related genes are regulated by these elicitors. Flavonoid biosynthetic pathway in higher plants has been well defined and several key enzymes involved in this pathway have been identified 1-3. Rutin is one of the most important flavonoids and plays a key role to protect plants against ultraviolet radiation or pathogens and is also used to prevent the side effects of some diseases such as cancer treatments, diabetes, and hypercholesteremia 4,5. The presence of rutin has been reported in many plant species, but only a limited number of plants such as Fagopyrum esculentum 6,7 and Cappris species, especially, C. spinosa 8 are identified as the biggest plant sources of rutin (or rutoside). C. spinosa L. (Capparidaceae) an aromatic plant, is widely distributed in the coastal areas of the Mediterranean basin and is reported with a 2.8% of rutin content in the leaf tissue 9 .

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 90%

mentioning

confidence: 99%

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Identification and tissue-specific expression of rutin biosynthetic pathway genes in Capparis spinosa elicited with salicylic acid and methyl jasmonate

Kianersi

Abdollahi

Mirzaie-Asl

et al. 2020

Sci Rep

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“…The polyamines in plants are not only found in cytoplasm, but also in speci ed organelles like mitochondria, chloroplasts, and vacuoles [104][105] to the current results, the content of compatible osmolytes and concentration of antioxidant enzymes were increased and decreased, respectively, with the application of 2.25 mM putrescine. There is evidence that SA causes a rise in the quantity of ROS in the cell, suggesting the existence of a self-induced SA 107 . This is not surprising, as a close correlation was recently reported between the endogenous PA and SA contents 108 , which may be responsible for the negative effects of greater concentrations of putrescine.…”

Section: Discussionmentioning

confidence: 99%

Improving water deficit tolerance of Salvia officinalis L. using putrescine

Mohammadi-Cheraghabadi

Modarres‐Sanavy

Sefidkon

et al. 2021

Preprint

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To study the effects of foliar application of putrescine (distilled water (0), 0.75, 1.5, and 2.25 mM) and water deficit stress (20%, 40%, 60%, and 80% available soil water depletion (ASWD)) on the physiological, biochemical, and molecular attributes of sage, a factorial experiment was performed in a completely randomized design with three replications in the growth chamber. The results of qRT-PCR analysis showed that putrescine concentration, irrigation regime, and the two-way interaction between irrigation regime and putrescine concentration significantly influenced cineole synthase, sabinene synthase, and bornyl diphosphate synthase relative expression. The highest concentration of 1,8-cineole, camphor, α-thujone, β-thujone, cineole synthase, sabinene synthase, and bornyl diphosphate synthase were obtained in the irrigation regime of 80% ASWD with the application of 0.75 mM putrescine. There was high correlation between expression levels of the main monoterpenes synthase and the concentration of main monoterpenes. The observed correlation between the two enzyme activities of APX and CAT strongly suggests they have coordinated action. On the other hand, the highest PO and SOD concentrations were obtained with the application of 0.75 mM putrescine under the irrigation regime of 40% ASWD. Putrescine showed a significant increase in LAI and RWC under water deficit stress. There was an increasing trend in endogenous putrescine when putrescine concentration was increased in all irrigation regimes. Overall, the results suggest that putrescine may act directly as a stress-protecting compound and reduced H2O2 to moderate the capacity of the antioxidative system, maintain the membrane stability, and increase secondary metabolites under water deficit stress.

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“…In addition, TP has a long history of use in traditional and folk medicine and it has multiple pharmacologic properties, such as anticancer, anti-inflammatory, cardiotonic, antispasmodic, an emmenagogue [28]. The known chemical constituents in TP are reported to include 3β-hydroxy parthenolide, canin and artecanin, having α-methylene butyrolactone moiety [29]. However, no data have been published on the inhibitory activities of TP toward RLAR, AGEs, sorbitol accumulation, and DPPH radical scavenging regulation.…”

Section: Discussionmentioning

confidence: 99%

Aldose Reductase, Protein Glycation Inhibitory and Antioxidant of Peruvian Medicinal Plants: The Case of Tanacetum parthenium L. and Its Constituents

et al. 2019

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Diabetes complications, including peripheral neuropathy, cataracts, impaired wound healing, vascular damage, arterial wall stiffening and retinopathy diseases, are among the most predominant health problems facing the world’s population today. The 22 Peruvian plant extracts were screened for their potential inhibitory activity against rat lens aldose reductase (RLAR) and DPPH radical scavenging. Among them, we have found that Tanacetum parthenium L. (TP) has the RLAR, AGEs and DPPH radical scavenging activities. We used for screening of active components in TP against RLAR and DPPH for the first time by ultrafiltration (UF) and DPPH. Compounds in TP were isolated by Sephadex column chromatography and their structures were established by MS and NMR spectroscopic analyses. Among the isolated compounds, ferulic acid, apigenin, luteolin-7-O-glucoside, luteolin, chrysosplenol, and kaempferol showed potent inhibition with IC50 values of 1.11–3.20 and 6.44–16.23 μM for RLAR and DPPH radical scavenging. Furthermore, these compounds suppressed sorbitol accumulation in rat lenses and ferulic acid, luteolin-7-O-glucoside, and luteolin have AGEs inhibitory activities with IC50 values of 3.43–6.73 μM. In summary, our study provides interesting plants for further study with respect to the treatment and prevention of diabetic complication of Peruvian plant and can provide the scientific base of the traditional uses.

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Parthenolide accumulation and expression of genes related to parthenolide biosynthesis affected by exogenous application of methyl jasmonate and salicylic acid in Tanacetum parthenium

Cited by 37 publications

References 46 publications

Identification and tissue-specific expression of rutin biosynthetic pathway genes in Capparis spinosa elicited with salicylic acid and methyl jasmonate

Identification and tissue-specific expression of rutin biosynthetic pathway genes in Capparis spinosa elicited with salicylic acid and methyl jasmonate

Improving water deficit tolerance of Salvia officinalis L. using putrescine

Aldose Reductase, Protein Glycation Inhibitory and Antioxidant of Peruvian Medicinal Plants: The Case of Tanacetum parthenium L. and Its Constituents

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