Partial characterisation of enterocin KP produced by Enterococcus faecalis KP, a cheese isolate

Abstract

Enterococcus faecalis KP, a bacteriocin‐producing strain, was identified using 16S rRNA gene sequence homology (99%). Enterocin KP from E. faecalis KP was sensitive to papain and ß‐mercaptoethanol, but resistant to trypsin, pepsin, lipase, catalase, α‐amylase, organic solvents, detergents, EDTA and heat treatment (90°C/30 min). It was active at a wide pH range (2.0–8.0) and produced at maximum level in de Mann Rogosa Sharpe broth at 32°C with an inoculum amount of 0.1–1.0% and an initial pH of 5.5–7.5. It was active against some Gram‐positive bacteria, including Listeria monocytogenes. It showed bactericidal activity against L. monocytogenes and its molecular weight was approximately 5.8 kDa.

Application of lactococcin BZ and enterocin KP against Listeria monocytogenes in milk as biopreservation agents

“…Brain Heart Infusion (BHI, Merck, Germany) broth was used for culturing L. monocytogenes ATCC 7644, and it was kept at -80 °C in BHI broth containing 20% glycerol. (ŞAHINGIL et al, 2011;ISLEROGLU et al, 2012). At the end of incubation, each culture was centrifuged (Beoco, Germany) at 7000×g for 20 min, and the supernatant was sterilized by membrane fi ltration (0.45 μm).…”

“…lactis BZ and Enterococcus faecalis KP, from traditionally produced Boza and White cheese (ŞAHINGIL et al, 2011;ISLEROGLU et al, 2012). Lactococcin BZ produced by L. lactis spp.…”

Enterosi̇n Kp’ni̇n İnhi̇bi̇tör Akti̇vi̇tesi̇ Üzeri̇ne Sodyum Klorür Ve Asi̇tli̇ği̇n Etki̇si̇

“…Starter kültür kullanılmaksızın geleneksel olarak üretilen Beyaz peynirden izole edilen Enterococcus faecalis KP tarafından sentezlenen enterosin KP'nin Listeria monocytogenes, L. ivanovii, Enterococcus faecium, E. faecalis gibi bazı gıda kaynaklı patojen bakterilere karşı antimikrobiyal aktiviteye sahip olduğu belirlenmiştir [19]. Bu çalışmanın amacı enterosin KP'nin inhibitör aktivitesi üzerine pH ve NaCl'ün etkisini belirlemektir.…”

Inhibitory effect of enterocin KP in combination with sublethal factors on Escherichia coli O157:H7 or Salmonella Typhimurium in BHI broth and UHT milk

“…Enterococcus faecalis KP was used as enterocin KP producer strain (Isleroglu et al, 2012). Salmonella Typhimurium RSSK 95091 and E. coli O157:H7 RSKK 232 were supplied by the Refik Saydam Hıfzıssıhha Culture Collection (Turkey).…”

“…Enterocin KP produced by Enterococcus faecalis KP exhibits bactericidal activity against some of the gram-positive pathogenic and spoilage bacteria such as Listeria monocytogenes, L. ivanovii, E. faecium, and E. faecalis (Isleroglu et al, 2012). Researchers reported that enterocin KP did not cause a clear inhibition zone against E. coli O157:H7 or Salmonella Typhimurium on BHI agar.…”