Partial characterization and immunomodulatory activity of polysaccharides from the stem of Dendrobium officinale (Tiepishihu) in vitro

Xia, Lin-Jing; Liu, Xiaofei; Guo, Huiyuan; Zhang, Hao; Zhu, Jun; Ren, Fazheng

doi:10.1016/j.jff.2011.12.006

Cited by 135 publications

(98 citation statements)

References 31 publications

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“…The molecular weights of polysaccharide were determined by the method described in previous research [15]. A high performance size elusion chromatography coupled with multi angle laser light scattering and refractive index (HPSEC-MALLS-RID) system was used.…”

Section: Molecular Weight Determinationmentioning

confidence: 99%

Structural features and immunostimulating effects of three acidic polysaccharides isolated from Panax quinquefolius

Wang

Yao

Sang

et al. 2015

International Journal of Biological Macromolecules

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Section: Molecular Weight Determinationmentioning

confidence: 99%

Structural features and immunostimulating effects of three acidic polysaccharides isolated from Panax quinquefolius

Wang

Yao

Sang

et al. 2015

International Journal of Biological Macromolecules

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“…The polysaccharide of Juniperus scopolorum showed potent immunostimulant effect, via increase of NO production and release of proinflammatory cytokines by macrophages (IL-1, IL-6, TNF-α) (Schepetkin et al, 2005). In addition, the polysaccharide obtained from Dendobrium officinal raised macrophage levels of TNF-α, IL-1β (He et al, 2016) and NO production (Xia et al, 2012). Based on these, we believe that EP-Mt might stimulate leukocytes to release chemotactic substances, being considered a promisor candidate to be used in immunostimulant therapies.…”

Section: Resultsmentioning

confidence: 99%

<b>Polysaccharide extract of <i>Mimosa tenuiflora</i> stem barks stimulates acute inflammatory response via nitric oxide

Silva-Leite

Queiroz

Madeira

et al. 2016

Acta Sci. Biol. Sci.

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ABSTRACT. Mimosa tenuiflora (Mimosaceae) or "jurema-preta" is well distributed in the northeast Brazil, being popularly used to treat skin lesions, burns and inflammation. The healing effect of the alcoholic extract prepared with its barks corroborates the popular use. This study aimed to evaluate the inflammatory response of polysaccharides extracted from M. tenuiflora barks (EP-Mt) by methanol/NaOH and ethanol precipitation. Inflammatory activity was assessed in rat models of acute inflammation (paw edema and peritonitis), by the following parameters: edema, vascular permeability, leukocyte migration, myeloperoxidase activity and pharmacological modulation of nitric oxide and prostaglandins. EP-Mt presented 3.8% yield, 41% carbohydrate and 0.34% protein. EP-Mt (0.01, 0.1, 1.0 mg kg -1 ) injected by subcutaneous route elicited paw edema that lasted from 30-420 min, with maximal effect at 1 mg kg -1 (40x vs. saline), and was inhibited by L-NAME (52%) and dexamethasone (26%). EP-Mt (1 mg kg -1 , via intraperitoneal) stimulated leukocytes migration (2.2x), mainly neutrophils (6.5x) and MPO activity (96%). The leukocyte migration elicited by EP-Mt was inhibited by dexamethasone (39%) and L-NAME (38%). EP-Mt containing high carbohydrate content induces acute inflammation via nitric oxide, which open perspectives of application in pathological conditions of immunosuppression.Keywords: Mimosaceae, medicinal plant, carbohydrate, inflammation.Extrato polissacarídico oriundo das cascas do caule de Mimosa tenuiflora induz resposta inflamatória aguda via óxido nítrico RESUMO. Mimosa tenuiflora (Mimosaceae) ou "jurema-preta", amplamente distribuída no nordeste brasileiro, é utilizada popularmente no tratamento de lesões de pele, queimaduras e inflamação. O efeito cicatrizante do extrato alcoólico de suas cascas corrobora o uso popular. Avaliou-se o efeito inflamatório dos polissacarídeos da casca de M. tenuiflora (EP-Mt), obtidos por extração com metanol/NaOH e precipitação com etanol. O efeito inflamatório foi avaliado em modelos agudos em ratos (edema de pata, peritonite) por meio dos seguintes parâmetros: edema, permeabilidade vascular, migração leucocitária, atividade da mieloperoxidase e modulação farmacológica de prostaglandinas e óxido nítrico. EP-Mt apresentou 3,8% de rendimento, 41% de carboidratos totais e 0,34% de proteína. EP-Mt (0,01, 0,1, 1,0 mg kg -1 ), administrado por via subcutânea, induziu edema de pata com duração de 30 a 420 min e efeito máximo na dose de 1 mg kg -1 (40x vs. salina), o qual foi inibido por L-NAME (52%) e dexametasona (26%). EP-Mt (1 mg kg -1 , via intraperitoneal) estimulou a migração de leucócitos (2,2x vs. salina), principalmente de neutrófilos (6,5x), com aumento da atividade da mieloperoxidase (96%). A migração de leucócitos foi inibida por dexametasona (39%) e L-NAME (38%). EP-Mt contendo elevado teor de carboidratos induz inflamação aguda via óxido nítrico com perspectivas de aplicação em condições patológicas de imunossupressão.Palavras-chave: Mimosaceae, plantas medicinais, carb...

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“…The content and quality of DDP are considered to the key indicators for evaluating the quality of Dendrobium officinale (Jin et al, 2016). DDP is the multi-hydroxyl polymer composed of glucose, mannose, galactose, glucose acid, glucose acid, xylose, Arabia sugar, galactose acid and other monosaccharide (Xia et al, 2012). This study observed the effect of DDP on the adipogenic differentiation of BMSCs.…”

Section: Discussionmentioning

confidence: 98%

Effects of Dendrobium officinale polysaccharide on adipogenic differentiation of rat bone marrow mesenchymal stem cells

Zhao

Hua

2017

Food Sci. Technol

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This study investigated the effect of Dendrobium officinale polysaccharide (DDP) on the adipogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs). DDP was extracted fresh Dendrobium officinale. Rat BMSCs were prepared, and then were treated with 0 (control), 50, 100, 200, 400, 800 μg/mL DDP, respectively. The cell viability was determined by MTT assay. The adipogenic differentiation was quantitatively analyzed by oil red D staining assay. The mRNA expressions of adipogenic differentiation related gene peroxisome proliferator-activated receptor gamma (PPARG), lipoprotein lipase (LPL) and fatty acid binding protein 4 (FABP4) were detected by RT-PCR. Results showed that, DDP with 0-800 μg/mL concentration had no significant toxicity to BMSCs. 200-800 μg/mL DDP could obviously inhibit the adipogenic differentiation of BMSCs. Compared with control group, the expression levels of PPARG, LPL and FABP4 mRNA 200, 400 and 800 μg/mL DDP groups were significantly decreased (P < 0.05 or P < 0.01). DDP can inhibit the adipogenic differentiation of BMSCs, which may be related with its down-regulation of PPARG, LPL and FABP4 expressions in BMSCs.Keywords: Dendrobium officinale; polysaccharide; bone marrow mesenchymal stem cells; adipogenic differentiation.Practical Application: Dendrobium officinale polysaccharide has an application prospect in treating the osteoporosis.

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Partial characterization and immunomodulatory activity of polysaccharides from the stem of Dendrobium officinale (Tiepishihu) in vitro

Cited by 135 publications

References 31 publications

Structural features and immunostimulating effects of three acidic polysaccharides isolated from Panax quinquefolius

Structural features and immunostimulating effects of three acidic polysaccharides isolated from Panax quinquefolius

<b>Polysaccharide extract of <i>Mimosa tenuiflora</i> stem barks stimulates acute inflammatory response via nitric oxide

Effects of Dendrobium officinale polysaccharide on adipogenic differentiation of rat bone marrow mesenchymal stem cells

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