Partial characterization of a novel bacteriophage of Vibrio harveyi isolated from shrimp culture ponds in Thailand

Pasharawipas, Tirasak; Thaikua, Surasak; Sriurairatana, Siriporn; Ruangpan, Lila; Direkbusarakum, Sataporn; Manopvisetcharean, Jaroon; Flegel, Timothy W.

doi:10.1016/j.virusres.2005.05.012

Cited by 68 publications

(70 citation statements)

References 16 publications

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“…VH1114 and its partner phage VHS1 were obtained from a Thai shrimp pond where black tiger prawns were being cultivated (15). All other bacterial isolates used were derived as subclones of this isolate.…”

Section: Media and Reagentsmentioning

confidence: 99%

“…However, phage production could not be sustained in culture, and the phage was lost, preventing further study. Later, another bacteriophage was isolated from shrimp culture ponds in Thailand, together with a partner V. harveyi isolate, VH1114 (15). It was an unenveloped virus with a long, noncontractile tail (100 to 120 nm long) and an isometric head (approximately 60 nm in diameter) that morphologically resembled the earlier Thai bacteriophage associated with virulence for shrimp.…”

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confidence: 99%

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Unstable Lysogeny and Pseudolysogeny in Vibrio harveyi Siphovirus-Like Phage 1

Khemayan

Pasharawipas

Puiprom

et al. 2006

Appl Environ Microbiol

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Exposure of Vibrio harveyi (strain VH1114) to V. harveyi siphovirus-like phage 1 (VHS1) resulted in the production of a low percentage of lysogenized clones of variable stability. These were retrieved most easily as small colonies within dot plaques. Analysis revealed that VHS1 prophage was most likely carried by VH1114 as an episome rather than integrated into the host chromosome. In the late exponential growth phase, lysogenized VH1114 continuously produced VHS1 but also gave rise to a large number of cured progeny. The absence of phage DNA in the cured progeny was confirmed by the absence of VHS1 DNA in Southern blot and PCR assays. Curiously, these very stable, cured subclones did not show the parental phenotype of clear plaques with VHS1 but instead showed turbid plaques, both in overlaid lawns and in dot plaque assays. This phenotypic difference from the original parental isolate suggested that transient lysogeny by VHS1 had resulted in a stable genetic change in the cured clones. Such clones may be called pseudolysogens (i.e., false lysogens), since they have undergone transient lysogeny and have retained some resistance to full lytic phage development, despite the loss of viable or detectable prophage.Luminous vibriosis and luminescent bacterial disease are terms that are used commonly to describe mortality caused mostly by pathogenic strains of Vibrio harveyi in hatcheries or rearing ponds of Penaeus species (6, 10). In serious epizootics, moribund shrimp emit a greenish luminescence due to bacterial infection or colonization. There is good evidence that exotoxins are involved in mortality from luminescent vibriosis, since extracellular products of cultured V. harveyi can be toxic when injected into Penaeus monodon (7,8) and since exotoxin production by V. harveyi is involved in shrimp larval disease (5).Upon examining the genetic relationship among virulent isolates of V. harveyi, Pizzutto and Hirst (17) found no discernible associations and thus proposed that virulence factors were transmitted by mobile elements such as transposons, plasmids, or bacteriophages. This led to a search for bacteriophages in Australian isolates of V. harveyi and to the eventual discovery of V. harveyi myo-like phage from a highly virulent V. harveyi isolate derived from diseased shrimp larvae (11-13). Phage conversion leading to toxin production occurs with many human-pathogenic bacteria (1, 2), including Vibrio cholerae (4), but had not previously been proven for V. harveyi.Association of V. harveyi virulence with a bacteriophage has also been reported in Thailand (14,19). However, phage production could not be sustained in culture, and the phage was lost, preventing further study. Later, another bacteriophage was isolated from shrimp culture ponds in Thailand, together with a partner V. harveyi isolate, VH1114 (15). It was an unenveloped virus with a long, noncontractile tail (100 to 120 nm long) and an isometric head (approximately 60 nm in diameter) that morphologically resembled the earlier Thai bacteriophage associa...

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Section: Media and Reagentsmentioning

confidence: 99%

mentioning

confidence: 99%

Unstable Lysogeny and Pseudolysogeny in Vibrio harveyi Siphovirus-Like Phage 1

Khemayan

Pasharawipas

Puiprom

et al. 2006

Appl Environ Microbiol

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Section: Vhs-1mentioning

confidence: 99%

“…It was stored in 3% NaCl or phosphate buffered saline (PBS) pH 7.0 at room temperature and amplified when required as previously described 11 . Approximately 20% of the VHS-1 genome has been sequenced and deposited at GenBank 11 . One of the deposited sequences PH102-1 (4668 bp, AF465603) contains an ORF called PH102-768 with significant sequence homology to DNA polymerase sequences at GenBank.…”

Section: Vhs-1mentioning

confidence: 99%

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Pasharawipas¹,

Thaikua²,

Flegel³

2009

ScienceAsia

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VHS1 (Vibrio harveyi siphoviridae like) is a newly described bacteriophage that has been tentatively placed in the family Siphoviridae based on its morphology by transmission electron microscopy. However, there are examples among emergent viruses where morphological characteristics do not correspond with genomic properties. Thus we attempted to verify the phylogenetic relationship of VHS1 to other tailed phages on the basis of its putative DNA polymerase. Although we found no clear support for placement of VHS1 in any tailed-phage family, we did discover that a general phylogenetic tree based on all putative tailed-phage sequences was not compatible with relationships based on phage morphology. However, construction of individual putative DNA polymerase trees for traditional morphological phage families revealed a clear tendency for grouping of putative DNA polymerase A and B types into either Gram negative or Gram positive bacteria hosts in a manner that varied with phage family. Based on these results, we propose that early branching of an ancestral DNA polymerase into polymerase A and B types may have accompanied phage specialization for Gram negative and Gram positive hosts. Whether this proposal is correct or not, the study suggests that comparisons based on major genes such as polymerases can constitute additional criteria for evaluation of DNA phage relationships and that genome comparisons may complement morphology in devising a more natural taxonomy.

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“…Although V. harveyi is non pathogenic to human, it is one of causative agents of the systemic fish disease, vibriosis, and sea food spoilage which occurs in many commercially important fish (20), including sharks, seahorse, lobster, shellfish or shrimps (13). Pseudosciaena crocea (big yellow croaker) is an important commercial marine fish in China, and has been widely cultured in hatcheries recent years.…”

Section: Introductionmentioning

confidence: 99%

Effect of combined function of temperature and water activity on the growth of Vibrio harveyi

Zhang¹,

Guo²,

Li³

et al. 2012

Braz. J. Microbiol.

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Vibrio harveyi is considered as a causative agent of the systemic disease, vibriosis, which occurs in many biological fields. The effects of temperatures (12.9-27.1 o C) and water activity (NaCl% 0.6%-3.4%) on V.harveyi were investigated. The behavior and growth characteristics of V. harveyi was studied and modeled.Growth curves were fitted by using Gompertz and Baranyi models, and the Baranyi model showed a better fittness. Then, the maximum growth rates (μ max ) and lag phase durations (LPD, λ) obtained from both Gompertz and Baranyi model were modeled as a combination function of temperature and water activity using the response surface and Arrhenius-Davey models for secondary model. The value of r 2 , MSE, bias and accuracy factor suggest Baranyi model has better fitness than Gompertz model. Furthermore, validation of the developed models with independent data from ComBase also shown better interrelationship between observed and predicted growth parameter when using Baranyi model.

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Partial characterization of a novel bacteriophage of Vibrio harveyi isolated from shrimp culture ponds in Thailand

Cited by 68 publications

References 16 publications

Unstable Lysogeny and Pseudolysogeny in Vibrio harveyi Siphovirus-Like Phage 1

Unstable Lysogeny and Pseudolysogeny in Vibrio harveyi Siphovirus-Like Phage 1

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Effect of combined function of temperature and water activity on the growth of Vibrio harveyi

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