Partial Characterization of Amylase Enzyme from Newly Isolated Bacterial Strain from Garden Soil

Abstract

Amylase producing bacterial strain MB2 was isolated from soil and it showed maximum production of enzyme in 48 hours. Crude enzyme has an optimum temperature of 40 o C and optimum pH of 7.0. The relative enzyme activity of 97% was observed at pH 6. After incubation for 40oC, the enzyme lost only 2.5% of activity after 48 hours of incubation.More than 90% of activity were retained even after 48 hours at pH 7. Calcium acts as an activator for amylase.