Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica

Abstract

The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100% identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands. The Streptomyces grew well in submerged system at 20°C, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and proteases were performing biosynthesis after nine days of submerged cultivation at 20°C. Newly Streptomyces were able to produce amylase and proteases in a cold environment. The ability to adapt to low temperature of these enzymes could make them valuable ingredients for detergents, the food industry and bioremediation processes which require low temperatures.

Screening of actinobacteria-producing amylolytic enzyme in sediment from Litopenaeus vannamei (Boone, 1931) ponds in Rembang District, Central Java, Indonesia

“…Another research from Kafilzadeh and Dehdari (2015) also found the potential of gnus Streptomyces isolated from mangrove sediment in amylase enzyme production. There has been reported research that Streptomyces from wide variety of environments like cold places (Antarctica) or associated with invertebrates were potential for the production of amylase enzyme (Cotarlet et al 2011;Khrisnakumar et al 2015). Research from Babu et al (2018) reported that some Streptomyces species such as Streptomyces coelicoflavus, Streptomyces diastaticus, Streptomyces parvus and Streptomyces champavatii were able to produce hydroliytic enzymes (protease, lipase, amylase, chitinase, pectinase, and ligninase) which further have potential as bioremediator agent in P. monodon culture system by the reduction of BOD level in the cultivation system.…”

Fungi from Admiralty Bay (King George Island, Antarctica) Soils and Marine Sediments

“…Besides, they have pharmaceutical and nanotechnological applications and can also be used for effluent treatment [13][14][15]. Hydrolytic enzymes such as proteases account for approximately 60% of the world enzyme trade and are used for treating industrial rejects and in the production of pharmaceuticals [16,17].…”