PARTIAL CHARACTERIZATION OF THE CHLOROPLAST GENOME FROM THE CHROMOPHYTIC ALGA <i>SYNURA PETERSENII</i> (SYNUROPHYCEAE)<sup>1</sup>

Wee, James L.; Chesnick, Joby Marie; Cattolico, Rose Ann

doi:10.1111/j.1529-8817.1993.tb00286.x

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…Genome size heterogeneity has been documented for some chromophyte ctDNA [ 16,52,601. Interestingly, the smallest size known for a chromophyte or rhodophyte ctDNA is from Synuru petersenii and is estimated to be 91.5 kb, close to the minimum size for the smallest chlorophyte ctDNA, Codium fragile at 89 kb [98].…”

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confidence: 82%

“…Although most of the methods used here have been described previously (Wee et al, unpubl. data) an overview is provided. Synurophyte DNA was obtained with CsClIEtBr gradient ultracentrifugation [98] from two clonal isolates (Synura glabra, Mallomonas annulata) and four clonal isolates (S. glabra, S. petersenii, S. echinulata, S. sphagnicola) for the psbA and ITS/ 5.8s studies, respectively. The ITSI5.8S region PCR primers were published previously [48] .…”

Section: Methodsmentioning

confidence: 99%

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Investigating the Comparative Biology of the Heterokonts with Nucleic Acids1

Wee

Hinchey

Nguyen

et al. 1996

J Eukaryotic Microbiology

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During the last decade investigations of heterokont protists utilizing molecular methods have challenged established biosystematic concepts. Most investigations emphasized the chloroplast genome or sequences from nuclear‐encoded, ribosomal genes. Refinement of DNA isolation protocols, advent of “universal primers” and the polymerase chain reaction, automated sequencing and increased accessibility of DNA sequence databases have expanded data‐gathering efficiency and increased dataset sizes. Because independent datasets have been easier to obtain, the testing of specific phylogenetic hypotheses has been facilitated, altering relationship concepts, primarily at phylum/class levels, and perceptions of cellular evolution. New approaches have emphasized ecological studies and extended studies to genus/species levels and poorly investigated genomes. This paper reviews studies documenting these impacts and identifies some current limitations. Additionally, new DNA sequence data from our laboratory on nuclear‐encoded rDNA internal transcribed spacers and the chloroplast‐encoded psb A gene suggest that these regions will provide taxonomic resolution for the Synu‐rophyceae, at the class/order level and subspecies level, respectively.

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confidence: 82%

Section: Methodsmentioning

confidence: 99%

Investigating the Comparative Biology of the Heterokonts with Nucleic Acids1

Wee

Hinchey

Nguyen

et al. 1996

J Eukaryotic Microbiology

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[13] Isolation of DNA from eukaryotic algae

Chesnick

Cattolico²

1993

Methods in Enzymology

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Phylogenetic analyses of the rbcL sequences from haptophytes and heterokont algae suggest their chloroplasts are unrelated

Daugbjerg

Andersen²

1997

Molecular Biology and Evolution

105

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Using the large subunit of RuBisCo (rbcL) sequences from cyanobacteria, proteobacteria, and diverse groups of algae and green plants, we evaluated the plastid relationship between haptophytes and heterokont algae. The rbcL sequences were determined from three taxa of heterokont algae (Bumilleriopsis filiformis, Pelagomonas calceolata, and Pseudopedinella elastica) and added to 25 published sequences to obtain a data set comprising 1,434 unambiguously aligned sites (approximately 98% of the total rbcL gene). Higher levels of mutational saturation in third codon positions were observed by plotting the pairwise substitutions with and without corrections for multiple substitutions at the same site for first and second codon positions only and for third positions only. In accordance with this finding phylogeny reconstructions were completed by omitting third codon positions, thus using 956 bp in weighted-parsimony and maximum-likelihood analyses. The midpoint-rooted phylogenies showed two major clusters, one containing cyanobacteria, glaucocystophytes, a phototrophic euglenoid, chlorophytes, and embryophytes (the green lineage), the other containing proteobacteria, haptophytes, red algae, a cryptophyte, and heterokont algae (the non-green lineage). In the nongreen lineage, the haptophytes formed a sister group to the clade containing heterokont algae, red algae, and the cryptophyte Guillardia theta. This branching pattern was well supported in terms of bootstrap values in weighted-parsimony and maximum-likelihood analyses (100% and 92%, respectively). However, the phylogenetic relationship among red algae, heterokonts, and a cryptophyte taxon was not especially well resolved. A four-cluster analysis was performed to further explore the statistical significance of the relationship between proteobacteria, red algae (including and excluding Guillardia theta), haptophytes, and heterokont algae. This test strongly favored the hypothesis that the heterokonts and red algae are more closely related to each other than either is to proteobacteria or haptophytes. Hence, this molecular study based on a plastid-encoded gene provides additional evidence for a distant relationship between haptophytes and the heterokont algae. It suggests an evolutionary scenario in which the ancestor of the haptophyte lineage engulfed a phototrophic eukaryote and, more recently, the heterokont lineage became phototrophic by engulfing a red alga.

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PARTIAL CHARACTERIZATION OF THE CHLOROPLAST GENOME FROM THE CHROMOPHYTIC ALGA SYNURA PETERSENII (SYNUROPHYCEAE)¹

Cited by 3 publications

References 27 publications

Investigating the Comparative Biology of the Heterokonts with Nucleic Acids1

Investigating the Comparative Biology of the Heterokonts with Nucleic Acids1

[13] Isolation of DNA from eukaryotic algae

Phylogenetic analyses of the rbcL sequences from haptophytes and heterokont algae suggest their chloroplasts are unrelated

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PARTIAL CHARACTERIZATION OF THE CHLOROPLAST GENOME FROM THE CHROMOPHYTIC ALGA SYNURA PETERSENII (SYNUROPHYCEAE)1

Cited by 3 publications

References 27 publications

Investigating the Comparative Biology of the Heterokonts with Nucleic Acids1

Investigating the Comparative Biology of the Heterokonts with Nucleic Acids1

[13] Isolation of DNA from eukaryotic algae

Phylogenetic analyses of the rbcL sequences from haptophytes and heterokont algae suggest their chloroplasts are unrelated

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PARTIAL CHARACTERIZATION OF THE CHLOROPLAST GENOME FROM THE CHROMOPHYTIC ALGA SYNURA PETERSENII (SYNUROPHYCEAE)¹