Partial Characterization of α-amylase Produced from Aspergillus niger using Potato Peel as Substrate

Mahmood, Shahzad; Shahid, Memuna Ghafoor; Irfan, Muhammad; Nadeem, Muhammad; Syed, Quratulain

doi:10.17582/pujz/2018.33.1.22.27

Cited by 12 publications

(8 citation statements)

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“…In the present study optimum pH was 5, poor growth of microbe in highly acidic and alkaline conditions is a well-known phenomenon, excepting the extremophiles (Kar & Ray 2008). Mahmood, Shahid, Nadeem, Irfan, and Syed (2016) reported that initial medium pH of 5 was best for amylase production by A. niger using potato peels as substrate in solid state fermentation. Various reports on optimization of parameters for amylase production have reported comparable results (Agrawal, Pradeep, Chandraraj, & Gummadi, 2005;Declerck et al, 2003;Kar & Ray 2008;Tamilarasan, Ashok, Abinandan, & Kumar, 2010).…”

Section: Model Validationmentioning

confidence: 99%

Potato peels: A potential food waste for amylase production

Mushtaq

Irfan

Tabssum

et al. 2016

J Food Process Engineering

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In the present study, thermophilic and amylase producing bacterium Bacillus subtilis K‐18 (KX881940) was isolated from local environment and identified by 16S rRNA gene sequencing technology. The amylase producing potential was checked by starch hydrolysis test using potato peels as carbon source. Central composite design (CCD) of response surface methodology (RSM) was used to optimize multiple process parameters such as substrate concentration, inoculum size, yeast extract, and pH. The optimized conditions obtained from RSM model were substrate concentration (2%), inoculum size (4%), yeast extract concentration (0.6%), and pH (5) yielding amylase activity of 1394.145 IU/ml after 24 hr of fermentation period at 50 °C. The maximum saccharification by indigenously produced amylase was 32.03% after 24 hr of incubation. The results confirmed that amylase produced by this newly isolated Bacillus subtilis K‐18 (KX881940) could be potent strain for industrial process particular for biofuel production. Practical application The enzyme produced in this study could be effectively used in hydrolysis of starch to produce sugars which could be widely used for the production of various chemicals such ethanol and other products through fermentation. This enzyme also has potential application in textile industry.

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Section: Model Validationmentioning

confidence: 99%

Potato peels: A potential food waste for amylase production

Mushtaq

Irfan

Tabssum

et al. 2016

J Food Process Engineering

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“…In the present study, potato peels waste shows considerably higher glucohydrolase production, as it is a rich source of carbohydrate and protein. Rapid microbial spoilage of potato peels waste grounds a serious disposal problem in the potato industry and could be solved by utilizing waste as a selective substrate for the production of starch degrading enzymes (Mahmood et al, 2016;Tamilarasan et al, 2010;Schieber and Saldana, 2009). Optimization of glucohydrolase production was performed under SSF using a fermentation medium containing soluble potato starch, organic nitrogen sources yeast extract and peptone, and Influence of incubation period on the production of glucohydrolase Incubation period plays an integral role in substrate utilization and enzyme production.…”

Section: Resultsmentioning

confidence: 99%

“…It is indicated from the graph that pH 5 is best for maximum enzyme production (Figure 3). Usually, it is desirable to use fungus, which will prefer pH values slightly acidic, to control contaminations with another organism (Kar & Ray, 2008;Mahmood et al, 2016).…”

Section: Influence Of Ph On Glucohydrolase Productionmentioning

confidence: 99%

Solanum tuberosum peel waste: an attractive substrate for Aspergillus sp. CP to produce glucohydrolase under solid state fermentation

2019

Aust. J. Basic & Appl. Sci

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Glucoamylase (EC 3.2.1.3) is exo-enzyme of extraordinary significance for saccharification of starchy materials and other related oligosaccharides. Glucoamylase successively hydrolyzes 1,4-alpha glycosidic bond from the non-reducing end of starch and other related polysaccharides and release free glucose, which then used as a feedstock for the process of fermentation (Gupta et al., 2003; Norouzian et al., 2006). Industrial production of glucoamylase has been performed by the fungi, Aspergillus niger (Wang et al., 2006). Glucoamylase has different applications in significant areas of food processing, animals feed, fermentation biotechnology, paper making, fabric texture, grain hydrolysis for the alcohol industry (Zambare, 2011). The production of glucoamylase from different Aspergillus species including Aspergillus niger, Aspergillus fumigatus,

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“…45 U/ml) after 72 h of incubation and then decreased. This decrease might be due to the unavailability of nutrients for large fungal population, and denaturation of the enzyme or due to production of acid byproducts (Mahmood et al, 2016). Many recent studies also reported that αamylase yield was maximum at 72 hrs of incubation at room temperature (Ahmed et al, 2015;Haq et al, 2002;Ramachandran et al, 2004;Mahmood et al, 2016).…”

Section: Effect Of Incubation Period On Enzyme Productionmentioning

confidence: 99%

Use of potato peel waste-a cost effective alternative substrate for the production of industrially useful α-amylase enzyme

Islam¹,

Mamun²,

Azad³

et al. 2020

JAFE

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Potato (Solanum tuberosum L.) is the fourth main crop worldwide for human consumption and widely used in food-processing industries, thus generating extremely large amounts of potato peel waste (PPW) as zero value by-product. This study was designed to utilize this PPW as biomaterials for the production of alpha amylase by submerged fermentation using Aspergillus oryzae, isolated from commercial white koji. The study results demonstrated that the maximum amylase production (30 U/ml) was achieved in PPW medium supplemented with yeast extract and salt after 72 hrs of incubation at ambient temperature (28 ± 2°C), pH 7.0 and 120 rpm agitation. Extraction of whole amylase from culture filtrate was found suitable with 80% ammonium sulfate saturation and maximum amylase activity of 42.07 U/ml was achieved after desalting. The purified α-amylase was found optimally active and stable up to 50°C.

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Partial Characterization of α-amylase Produced from Aspergillus niger using Potato Peel as Substrate

Cited by 12 publications

References 0 publications

Potato peels: A potential food waste for amylase production

Potato peels: A potential food waste for amylase production

Solanum tuberosum peel waste: an attractive substrate for Aspergillus sp. CP to produce glucohydrolase under solid state fermentation

Use of potato peel waste-a cost effective alternative substrate for the production of industrially useful α-amylase enzyme

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