Partial Coated Stem Cells with Bioinspired Silica as New Generation of Cellular Hybrid Materials

Abstract

The manipulation of cell surfaces in anchorage-dependent cells can help overcome difficulties presented during cell handling and application. Silica-based protective mechanisms existing in free-floating microorganisms may be used as inspiration for sustaining human cell survival in suspension. Gathering on this, herein human adipose-derived mesenchymal stem cells (hASCs) are partially coated with a hard silica layer that operates as a supporting platform for individual cells in suspension. Inspired by the organic templates involved in biosilicification, a novel chitosan (CHT) derivative displaying fully natural quaternary amine moieties is synthetized via a rapid, one-pot strategy. Silicification is promoted on individual hASCs surfaces via a two-step process: a priming step onto previously adhered cell with this CHT derivative, followed by a biocompatible sol-gel process. hASCs holding a silica backpack exhibit enhanced cell survival in suspension conditions and can spread and acquire a more adherent phenotype. This new protocol for cell-surface modification also provides a new generation of hybrid materials with functionalization of the silica backpack, which can be applied to different areas such as tissue engineering, biosensing, drug delivery, and targeted cell-based therapies.

A Critical Review on Polymeric Biomaterials for Biomedical Applications

“…It is important to keep the cell structures intact during the whole 3D printing process. Earlier studies have shown the effect of coating on protecting the cells from the damage that occurs due to the stresses generated during the printing process [ 121 , 122 ]. In a study, adipose derived mesenchymal stem cells were partially coated with silica, inspired by biosilicification [ 122 ].…”

“…Earlier studies have shown the effect of coating on protecting the cells from the damage that occurs due to the stresses generated during the printing process [ 121 , 122 ]. In a study, adipose derived mesenchymal stem cells were partially coated with silica, inspired by biosilicification [ 122 ]. Individual cells were coated with a hard silica layer (called backpack) to provide them with a supporting platform [ 122 ].…”

“…In a study, adipose derived mesenchymal stem cells were partially coated with silica, inspired by biosilicification [ 122 ]. Individual cells were coated with a hard silica layer (called backpack) to provide them with a supporting platform [ 122 ]. The two-step process involving bioinspired priming and silicification used Tetra ethyl ortho silicate as the source of silica [ 122 ].…”

“…Individual cells were coated with a hard silica layer (called backpack) to provide them with a supporting platform [ 122 ]. The two-step process involving bioinspired priming and silicification used Tetra ethyl ortho silicate as the source of silica [ 122 ]. Characterization of cells yielded good results in terms of factors such as cell viability and cytocompatibility.…”

“…Characterization of cells yielded good results in terms of factors such as cell viability and cytocompatibility. This has also generated significant interest for their use in tissue engineering, targeted drug delivery, and biosensing applications [ 122 ].…”