Partial collapse of DNA tetrahedron for miRNA assay with duplex-specific nuclease-assisted amplification

Abstract

Partial disassembly of DNA tetrahedron is designed for the highly sensitive electrochemical detection of target miRNA, which is triggered by target and amplified by duplex-specific nuclease.

Real-time in situ fluorescence imaging of telomerase and miR378 in living cells using a two-color DNA tetrahedron nanoprobe combined with molecular beacons

“…4 Abnormal miRNA expression is commonly found in cancer cells; therefore, miRNAs can be clinically used as diagnostic and prognostic biomarkers of cancers. Currently, many methods can be used to detect miRNAs, such as polymerase chain reaction, 5 electrohydrodynamic migration microfluidic technology, 6 colorimetric assays, 7 electrochemical methods, 8,9 and surface plasmon resonance. 10 However, most of these methods require sample extraction and do not allow intracellular molecular detection and bioimaging.…”

The Current Progress of Tetrahedral DNA Nanostructure for Antibacterial Application and Bone Tissue Regeneration

“…Currently, TDNs-based biosensors have gained widespread application in anchoring small molecules, nucleic acids, proteins, and cells to specific sites due to their high specificity, rapid analysis, accuracy, and cost-effectiveness. [27][28][29][30] For instance, Miao et al developed an ultrasensitive electrochemical system using TDN-modified AuNPs tags that can distinguish targeted miRNA from interfering miRNAs with slightly different sequences. 31 TDNs-based nanoplatforms have also shown potential in clinical diagnosis, as exemplified by the use of DNA walking nanomachine on a tetrahedral DNA scaffold interface for ultrasensitive electrochemical analysis of miRNA (miR-141), a stable serum-based biomarker for prostate cancer diagnosis.…”

Dual cascade nucleic acid recycling-amplified assembly of hyperbranched DNA nanostructures to construct a novel plasmonic colorimetric biosensing method

“…31,32 Moreover, certain nucleic acids with specific base sequences can also assemble into various DNA nanomaterials with controlled structure, excellent biocompatibility and feasible functionalization for the biosensing signal amplification application. [33][34][35] This suggests that the combination of the LSPR property regulation with the aptamer recognition-based nucleic acid amplification should be an effective approach to improve the performances of conventional colorimetric biosensors.…”