PARTIAL DEIODINATION OF L-THYROXINE<sup>1</sup><sup>2</sup>

Flock, Eunice V.; Bollman, Jesse L.; Grindlay, John H.; Stobie, George H.

doi:10.1210/endo-69-3-626

Cited by 33 publications

(16 citation statements)

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“…Subsequently, many investigators have detected the pres ence of the acetic analogues in the b:Ue of animals previously injected with *T3 or *T4. Among these investigators were Flock,et al (25,27) who demonstrated the analogues in dog bile. Galton and PittRivers (36) identified the acetic acid analogues of T4 and r 3 in the liver of mice, 12-48 hours after the mice had received rl31 intraperitoneally.…”

Section: Metabolism and Excretion Of Thyroid Hormones By The Livermentioning

confidence: 99%

“…This solvent has been used extensively by Flock,et al,(27,29) in a 4:1:5 ratio and by Nakano (73) in a 4:1:2 ratioo n was used i.."'l this work because of its ability to resolve metabolites into narrow bands. Barker(?)…”

Section: Chromatographic Proceduresmentioning

confidence: 99%

Section: Hydroly~is Of the Conjugated Rl31 Compoundsmentioning

confidence: 99%

“…In the dog, *TJ and *T 4 were excreted more rapidly-in urine than in bile while the radioactivity of T 3 labeled with 1131 was exel"eted more rapidl.y than *T4 (Flock,et al,25,27)o Although no literature was located which was related to the rate of excretion of *T:3 to *T4 in man, Keati:rig and Albert (56) observed that little radioactivity from T4 was excreted into the feces and that the major pathway of excretion of *T4 was in the urine. In general, urinary elimination of the tllyroid hormones exceed biliary elimination in all mammals which have been studied with the excaption of the rodents.…”

Section: · Of Urine Excreted In Four Hoursmentioning

confidence: 99%

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Metabolism and excretion of thyroxine and triiodothyronine in chickens

HUTCHINS

NEWCOMER

1966

General and Comparative Endocrinology

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Section: Metabolism and Excretion Of Thyroid Hormones By The Livermentioning

confidence: 99%

Section: Chromatographic Proceduresmentioning

confidence: 99%

Section: Hydroly~is Of the Conjugated Rl31 Compoundsmentioning

confidence: 99%

Section: · Of Urine Excreted In Four Hoursmentioning

confidence: 99%

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Metabolism and excretion of thyroxine and triiodothyronine in chickens

HUTCHINS

NEWCOMER

1966

General and Comparative Endocrinology

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“…Column chromatography (Flock,et al.,27) was of special vaJ.ue because of greater sample capacity. Following the procedure as outlined by Braasch,et al (14) and Gross and Pitt~R:i.vers (43), a kieselguhr column was prepared by first heating the kieselguhr for four hours at 40o 0 c. In order to determine which hormone was degraded and excreted at the greater rate, the percent of 1 1 3 1 excreted in four hours into urine and bile from *T4=injected birds was compared with that from urine and bile respectively collected from birds injected with *T 3 (Table III).…”

Section: Hydroly~is Of the Conjugated Rl31 Compoundsmentioning

confidence: 99%

Metabolism and excretion of thyroxine and triiodothyronine in chickens

Hutchins

Newcomer

1966

General and Comparative Endocrinology

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Effects of Thyroid on Permeability, Composition, and Electrolyte Metabolism Of Brain and Other Tissues

Raskin¹,

Fishman²

1966

Archives of Neurology

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THE .HE SYNDROMES that may result from a great excess or deficiency of thyroid hormone in man, thyroid storm,1 and myxedema coma,2 are both characterized neurologically by apathy, somnolence, psychosis, confusion, and, ultimately, coma\p=m-\ clinical expressions of profound alterations in cerebral function. The pathophysiology of these metabolic encephalopathies is poorly understood and, though nonspecific atrophic changes have been described in adult hypothyroid brains,3 these are inconsistent ; no definite lesions have been observed in hyperthyroid brain.4 Although brain oxygen consumption has been shown to be diminished in human myxedema,5 it is normal in thyrotoxicosis.6 This study was undertaken, then, to elucidate thyroidal effects upon brain function. Myxedema and thyrotoxicosis were simulated in rats by thyroidectomy and thyroxine intoxication; brain permeability, composition and electrolyte metabolism were studied using sucrose 14C, sulfate 35S, sodium 24 and potassium 42. MethodsAdult male Sherman rats were used in all experiments. The following isotopically labeled com¬ pounds were obtained from the New England Nuclear Co., Boston : universally labeled sucrose "C (specific activity, 360/ic/mM), sodium sulfatê S (specific activity, 165y¿c/mM), and, from Iso-Serve, Boston, sodium chloride 2iNa (specific ac¬ tivity, 1560/íc/gm Na), and potassium chloridê K (specific activity, 184/ic/gm). Rats were assumed to be hyperthyroid after re¬ ceiving 10 intraperitoneal injections of L-thyroxine, 40/ig/100 gm body weight, over an 11-day period. Hypothyroid rats were produced by thyroidectomy, 6 to 12 weeks elapsing prior to study. The para¬ thyroid glands were not preserved ; therefore, rats operated upon were given 200 units of vitamin D p.o., b.i.w. and 0.5% calcium lactate-tap water solution was used as drinking water. At the time the animal was killed, the plasma calcium level of this population, 4.75±0.5 (SD) mEq/liter,8 did not differ significantly from the controls, 5.1 ±0.4 (SD) mEq/liter.' All injections of isotopes were made intraperitoneally in 1-ml volumes (excluding the microflow pump experiments, vi). Constant blood levels of sucrose 14C and sulfate^S were maintained by nephrectomizing the animals prior to injection of isotope for experiments up to six hours in duration. However, because the chemical changes of uremia, ie, azotemia, become manifest beyond this time period in the nephrectomized rat,' a microflow pump syringe (Sage Instrument Co White Plains, NY) was used to maintain relatively constant blood levels of isotope for periods up to 48 hours. This device is a glass Luer-Lok syringe (Fig 1) to which is tightly fitted a battery case with electrodes that protrude into the hollow piston within the syringe. The piston is filled with a hydrazine solu¬ tion which emits nitrogen and hydrogen when electrolyzed, thus forcing the piston down, emitting the isotopie solution from the syringe at a linear rate proportional to the rate of electrolysis in the piston. -Electrode -Gosket _Flow Rate Setter e (R...

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PARTIAL DEIODINATION OF L-THYROXINE¹²

Cited by 33 publications

References 0 publications

Metabolism and excretion of thyroxine and triiodothyronine in chickens

Metabolism and excretion of thyroxine and triiodothyronine in chickens

Metabolism and excretion of thyroxine and triiodothyronine in chickens

Effects of Thyroid on Permeability, Composition, and Electrolyte Metabolism Of Brain and Other Tissues

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PARTIAL DEIODINATION OF L-THYROXINE12

Cited by 33 publications

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Metabolism and excretion of thyroxine and triiodothyronine in chickens

Metabolism and excretion of thyroxine and triiodothyronine in chickens

Metabolism and excretion of thyroxine and triiodothyronine in chickens

Effects of Thyroid on Permeability, Composition, and Electrolyte Metabolism Of Brain and Other Tissues

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PARTIAL DEIODINATION OF L-THYROXINE¹²