Partial Interfertility Among theCeratocystisSpecies on Conifers

Harrington, T. C.; McNew, Douglas

doi:10.1006/fgbi.1998.1083

Cited by 28 publications

(29 citation statements)

References 22 publications

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“…Incomplete reproductive isolation has been reported for other examples of fungal speciation [36][37][38]. Partial interfertility sometimes occurs between distinct species [39], but usually produces fewer ascospores with a decreased ascospore germination percentage than that seen at intraspecific level [40].…”

Section: Discussionmentioning

confidence: 99%

Ophiostoma tsotsi sp. nov., A Wound-infesting Fungus of Hardwood Trees in Africa

et al. 2010

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Polymorphic sequence-characterised marker assays from a recent diversity study on the Ascomycete fungus Ophiostoma quercus reported that some isolates from Africa were genetically distinct from O. quercus. In the present study, these African isolates were compared with authentic O. quercus isolates by evaluating morphological characters, growth in culture, mating compatibility and DNA sequence data. The isolates from Africa were morphologically similar to O. quercus, presenting Pesotum and Sporothrix synanamorphs in culture. Phylogenetic analyses of the ribosomal internal transcribed spacer regions 1 and 2, b-tubulin and translation elongation factor 1-a gene regions confirmed that the African group represents a distinct species within the hardwood lineage of the O. piceae complex, closely related to O. ulmi and O. himalulmi. Mating studies between O. quercus and the African isolates showed that isolates mated predominantly with those of their own group, although there were rare cases of fertile crosses between the groups. Isolates residing in the African lineage are described here as a new species, O. tsotsi sp. nov.

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Section: Discussionmentioning

confidence: 99%

Ophiostoma tsotsi sp. nov., A Wound-infesting Fungus of Hardwood Trees in Africa

et al. 2010

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“…If ascospore progeny were recovered, the colonies were generally abnormal, fluffy (abundant aerial mycelium) and showed restricted growth. Such progeny are typical of interspecific hybridizations in other Ceratocystis species (Harrington and McNew 1998).…”

Section: Resultsmentioning

confidence: 89%

“…coeruiescens species complex (Harrington and McNew 1998). In this case, some progeny from interspecific pairings had two parental isozyme electromorphs.…”

Section: Discussionmentioning

confidence: 89%

“…Intersterility among closely related Ceratocystis species has been more thoroughly studied in the C. coerulescens complex (Harrington and McNew 1998) and particularly between C. poionica and C. iaricicola .…”

Section: Observations On Intersterilitymentioning

confidence: 99%

“…Procedures for intersterility tests were similar to those of Harrington and McNew (1998). Tester strains were grown on MYEA at room temperature (about 22-24 C).…”

Section: Fungalmentioning

confidence: 99%

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Host specialization, intersterility, and taxonomy of populations of Ceratocystis fimbriata from sweet potato, sycamore, and cacao

Engelbrecht

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Deletion of the MAT- 2 mating-type gene during uni-directional mating-type switching in Ceratocystis

Witthuhn¹,

Harrington

Wingfield

et al. 2000

Current Genetics

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Ceratocystis eucalypti is strictly heterothallic, with single ascospore strains representing one of two opposite mating types. Most other Ceratocystis species, including C. virescens, C. pinicola, and C. fimbriata, are homothallic. In the homothallic species, the MAT-2 strains are self-fertile, while MAT-1 strains are self-sterile and grow more slowly than MAT-2 strains. The current hypothesis is that self-fertility of MAT-2 strains is due to the deletion of the MAT-2 mating-type gene, resulting in the expression of the MAT-1 mating type. These mutant MAT-1 strains are able to cross with MAT-2 strains. Part of the MAT-2 mating-type gene in C. eucalypti, C. pinicola, and C. fimbriata was amplified using degenerate primers designed from the conserved MAT-2 HMG DNA-binding motif. The expected approximately 300-bp PCR products were cloned and sequenced. Specific primers were designed that amplified 210-bp fragments only in MAT-2 isolates of C. eucalypti, C. virescens, C. pinicola, and C. finbriata. These fragments were present in self-fertile field isolates and self-fertile progeny but were absent in the self-sterile (MAT-1) progeny from selfings of C. virescens, C. pinicola, and C. fimbriata, thus supporting the hypothesis that the MAT-2 mating-type gene is deleted during uni-directional mating-type switching. A Southern-blot analysis was performed to confirm the deletion of MAT-2 gene in self-sterile progeny. The DNA sequence data for the C. eucalypti MAT-2 mating-type gene was increased to 1371-bp using TAIL-PCR and uneven PCR, representing a portion of the complete MAT-2 gene DNA sequence.

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Partial Interfertility Among theCeratocystisSpecies on Conifers

Cited by 28 publications

References 22 publications

Ophiostoma tsotsi sp. nov., A Wound-infesting Fungus of Hardwood Trees in Africa

Ophiostoma tsotsi sp. nov., A Wound-infesting Fungus of Hardwood Trees in Africa

Host specialization, intersterility, and taxonomy of populations of Ceratocystis fimbriata from sweet potato, sycamore, and cacao

Deletion of the MAT- 2 mating-type gene during uni-directional mating-type switching in Ceratocystis

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