Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequences

Rubin, Carol M.; Houck, Catherine M.; Deininger, Prescott L.; Friedmann, Theodore; Schmid, Carl W.

doi:10.1038/284372a0

Cited by 324 publications

(139 citation statements)

References 11 publications

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“…Freshly dissected rabbit and mouse ovaries were embedded using the same protocol. DNA-DNA in situ hybridization was as described previously [18][19]. Briefly, tissues were sectioned 5 mm, digested in proteinase K (10 mg/ml) at 37 o C for 15 min, and post-fixed in 0.4% paraformaldehyde.…”

Section: Dna Genotypingmentioning

confidence: 99%

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Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes

Chen¹,

Liu

et al. 2003

Cell Res

200

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To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PCR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well as mixed cell populations that express markers representative of all three germ layers. Thus, ntES cells derived from human somatic cells by NT to rabbit eggs retain phenotypes similar to those of conventional human ES cells, including the ability to undergo multilineage cellular differentiation.

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Section: Dna Genotypingmentioning

confidence: 99%

“…ntES cell colonies were hybridized to probes specific for Alu repeats in the human genome [18,19]. Alu probes specifically stained nuclei of ntES cells (Fig 7).…”

Section: Isolation and Characterization Of Ntes Cellsmentioning

confidence: 99%

Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes

Chen¹,

Liu

et al. 2003

Cell Res

200

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“…They are transcribed into HnRNA and represent the prevalent class of repetitive RNA (16). The primary nucleotide sequence of individual cloned members of the human Alu family diverge from each other about 10 to 20 percent (17,18).…”

Section: Introductionmentioning

confidence: 99%

“…A recombinant library of monkey genomic DNA in X bacteriophage was screened with a cloned member of the human Alu family (BLUR 8, ref. 16,17), and the arrangement of Alu-like sequences was investigated in several clones selected from the monkey library. The clones analyzed were not initially chosen for the presence of Alu-like sequence, but because they contained other DNA sequences being investigated in this laboratory.…”

Section: Introductionmentioning

confidence: 99%

Interspersed repeated sequences in the African green monkey genome that are homologous to the human Alu family

1981

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The dominant family of interspersed repetitive DNA sequences in the human genome has been termed the Alu family. We have found that more than 75% of the X phage in a recombinant library representing an African green monkey genome hybridize with a human Alu sequence under stringent conditions. A group of clones selected from the monkey library with probes other than the Alu sequence were analyzed for the presence and distribution of Alu family sequences. The analyses confirm the abundance of Alu sequences and demonstrate that more than one repeat unit is present in some phages. In the clones studied, the Alu units are separated by an average of 8 kilobase pairs of unrelated sequences. The nucleotide sequence of one monkey Alu sequence is reported and shown to resemble the human Alu sequences closely. Hence, the sequence, dispersion pattern, and copy number of the Alu family members are very similar in the African green monkey and human genomes.Among the clones investigated were two that contain segments of the satellite DNA termed o-component joined to non o-component DNA. The experiments indicate that in the monkey genome Alu sequences can occur close to regions of a-component DNA.

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“…SINEs were initially found in primates and rodents (Krayev et al 1980;Rubin et al 1980); now it is generally accepted that several SINE families are present in most (probably all) higher eukaryotes. Usually, one SINE family has over 100,000 copies per haploid genome, while others are less abundant [e.g., the mouse genome contains ∼100,000 B1s and B2s each (Kramerov et al 1979) and ∼40,000 IDs (Kass et al 1996)].…”

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confidence: 99%

CAN—a pan-carnivore SINE family

Vassetzky

Крамеров

2002

Mammalian Genome

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Abstract. Short retroposons or short interspersed elements (SINEs) constituting 5-10% genome have been isolated from various organisms. CAN SINEs initially found in American mink were named after dogs (Canis), and the range of their distribution in the genomes of carnivores and mammals in general remained topical. Here we demonstrate CAN sequences in representatives of all carnivore families, but not beyond carnivores, on the basis of sequence bank search and genomic PCR. Analysis of their distribution supports division of carnivores into caniform (dogs, mustelids, raccoons, bears, and pinnipeds) and feliform (cats, civets, and hyenas) lineages. CAN structure is considered in the context of their function and evolution.A significant portion of the eukaryotic genome is composed of mobile elements propagated by retroposition, a process involving transcription and reverse transcription (Rogers 1985). Long retroposons (LINEs) code for the activities required for retroposition (reverse transcriptase and endonuclease), while short retroposons (SINEs) lack these.SINEs are genomic repeats 80-400 bp long, apparently originating from RNA (more commonly tRNA); a typical SINE consists of three regions; a tRNA-related region, a tRNA-unrelated region, and an A-rich region. The tRNA-related region contains an internal promoter of RNA polymerase III and provides for its transcription (Daniels and Deininger 1985;Jagadeeswaran et al. 1981). Owing to the mechanism of amplification, the 3Ј-part of SINEs is A-rich, and the genomic elements are flanked by short direct repeats.SINEs can be classified as members of several superfamilies sharing structural similarity, apparently inherited from a common ancestor [e.g., members of Alu/B1 superfamily originate from an ancient element FAM (Quentin 1992)]. The vast majority of SINE copies in the genome are incapable of active amplification, and the process is due to a few master sequences as indicated by the presence of distinct subfamilies and the activity of one or a few at any given time.

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Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequences

Cited by 324 publications

References 11 publications

Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes

Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes

Interspersed repeated sequences in the African green monkey genome that are homologous to the human Alu family

CAN—a pan-carnivore SINE family

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