Partial pretreatment of ingredient mixture effectively improved feed chemical composition, physicochemical properties and in vitro digestibility

Hahor, Waraporn; Thongprajukaew, Karun; Nuntapong, Nutt; Saekhow, Suktianchai; Rungruangsak‐Torrissen, Krisna; Dumrongrittamatt, Terdtoon; Phonchai, Apichai

doi:10.1016/j.anifeedsci.2022.115216

Cited by 7 publications

(14 citation statements)

References 45 publications

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“…Since in vitro digestibility correlates with nutrient bioavailability 59 – 61 , our investigations suggest the potential of R. paludigena CM33 as an animal feed supplement, especially for aquatic animals. However, detailed composition, such as amino acid profiles, should be clarified before being used as an alternative feed supplement.…”

Section: Resultsmentioning

confidence: 90%

Enhanced high β-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals

Thumkasem,

On-mee,

Kongsinkaew

et al. 2024

Sci Rep

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This study assessed Rhodotorula paludigena CM33's growth and β-carotene production in a 22-L bioreactor for potential use as an aquatic animal feed supplement. Optimizing the feed medium's micronutrient concentration for high-cell-density fed-batch cultivation using glucose as the carbon source yielded biomass of 89.84 g/L and β-carotene concentration of 251.64 mg/L. Notably, using sucrose as the carbon source in feed medium outperforms glucose feeds, resulting in a β-carotene concentration of 285.00 mg/L with a similar biomass of 87.78 g/L. In the fed-batch fermentation using Sucrose Feed Medium, R. paludigena CM33 exhibited high biomass production rates (Qx) of 0.91 g/L.h and remarkable β-carotene production rates (Qp) of 2.97 mg/L.h. In vitro digestibility assays showed that R. paludigena CM33, especially when cultivated using sucrose, enhances protein digestibility affirming its suitability as an aquatic feed supplement. Furthermore, R. paludigena CM33's nutrient-rich profile and probiotic potential make it an attractive option for aquatic nutrition. This research highlights the importance of cost-effective carbon sources in large-scale β-carotene production for aquatic animal nutrition.

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Section: Resultsmentioning

confidence: 90%

Enhanced high β-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals

Thumkasem,

On-mee,

Kongsinkaew

et al. 2024

Sci Rep

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“…Before selecting appropriate ingredients and feed preparation procedures, previous studies of aquatic animals determined the digestibility of protein and carbohydrate in vitro, based on extracted digestive enzymes (Sansuwan et al, 2017; Thongprajukaew et al, 2011). This technique for assessing the bioavailability of protein and carbohydrate (Hahor et al, 2022) is practical, inexpensive, comparatively rapid, and a lot of reactions can be performed at the same time to effectively predict in vivo growth (Mohapatra et al, 2003; Rungruangsak‐Torrissen et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

First successful head‐start program of leatherback sea turtles (Dermochelys coriacea) in Thailand and proposed dietary strategy

Kanghae,

Thongprajukaew,

Suraswadi

et al. 2023

Zoo Biology

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Leatherback sea turtle (Dermochelys coriacea) hatchlings have previously been kept alive for over a year in captive conditions but these were the few survivors of initially larger groups. In this report, newly hatched leatherback sea turtles were randomly harvested from two nests in Thailand and successfully reared before release back to the wild. In captivity, the survival of the turtles decreased sharply during the first 4 or 5 months, and then steadied until the end of the 12‐month trial. The survival of yearlings from one nest was 13.3% and from the other nest 46.7%. Their growth was exponential, reaching 1.36−3.35 kg (n = 9) at the end of program. The causes of death of the reared turtles were accidents (25.0−38.5%) and infections from water and live food (61.5−75.0%). The dietary requirements of the turtles were investigated by determining the digestibility of protein and carbohydrate in vitro, using enzymes from the digestive tracts of hatchlings that had died in an accident (70.1−92.9 g body weight). The results indicated that the most suitable source of protein was wheat gluten, followed by soybean meal, nonruminant meat and bone meal, fish meal, nonruminant meat meal, pork meal, and skimmed milk, while suitable carbohydrate sources were mashed cassava, corn, wheat flour, and alpha starch, followed by cereal meal and rice flour. Findings from the current report provide a practical protocol for head‐starting leatherback sea turtles, and indicate the possible basis of an effective artificial diet for this species.

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“…Fourier-transform infrared spectrometer (FTIR8400s; Shimadzu, Kyoto, Japan) was used to qualitatively describe the change in nutritive values due to pretreatment methods. The technique and program were run as described by Hahor et al [ 17 ] and Thongprajukaew et al [ 18 ] with some modifications. The 2 mg of powdered samples were mixed with 200 mg of KBr in a vacuum dryer for 24 h, and then the mixture was uniformly ground and pressed into a tablet.…”

Section: Methodsmentioning

confidence: 99%

“…Pretreatment procedures can significantly influence the physicochemical properties of starch, thereby impacting its quality [ 16 ]. The desirable effects from material pretreatments can be traced by properties in aspects to enhance enzymatic hydrolysis in vitro, such as pH, water solubility, water absorption capacity (WAC), thermal properties, diffraction pattern and relative crystallinity, and microstructure [ 17 , 18 , 19 ]. In vitro digestibility can be applied to assess bioavailability in animals.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro digestibility can be applied to assess bioavailability in animals. This practical, inexpensive technique is comparatively rapid and can perform multiple reactions simultaneously, effectively predicting in vivo growth trials [ 17 , 20 ]. These observed parameters might suggest a suitable protocol for the pretreatment of cassava chips prior to use in animal feeds.…”

Section: Introductionmentioning

confidence: 99%

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Physical Pretreatments of Cassava Chips Influenced Chemical Composition, Physicochemical Properties, and In Vitro Digestibility in Animal Models

Takaeh,

Poolthajit,

Hahor

et al. 2024

Animals

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Physical pretreatment procedures can significantly influence the quality of food and feed raw materials. To increase the ability to be digested in animals, cassava chips were pretreated by four alternative methods (extrusion, microwave irradiation, gamma irradiation, or NaOH hydrolysis), and then the chemical composition, physicochemical properties, and in vitro digestibility of the pretreated samples were assessed and compared with unprocessed cassava chips (control). The chemical compositions (crude protein, ether extract, neutral detergent fiber, acid detergent fiber, ash, non-fiber carbohydrate, and gross energy) were significantly altered due to the pretreatment methods (p < 0.05). The nutritive profile was qualitatively changed when assessed through Fourier-transform infrared spectroscopy. Some physicochemical properties in association with enzymatic hydrolysis, which include pH, water solubility, water absorption capacity, thermal properties (differential scanning calorimetry), diffraction pattern (X-ray diffractometry), and microstructure (scanning electron microscopy), were significantly changed. In vitro carbohydrate digestibility based on digestive enzyme extracts from Nile tilapia (Oreochromis niloticus) suggests the microwaving method for cassava chips preparation, while microwaving, followed by extrusion or gamma irradiation, was suggested for broiler (Gallus gallus domesticus). There were no differences in the pepsin-cellulase digestibility values tested for the ruminant model. These findings suggest the use of pretreated cassava chips in animal feeding.

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Partial pretreatment of ingredient mixture effectively improved feed chemical composition, physicochemical properties and in vitro digestibility

Cited by 7 publications

References 45 publications

Enhanced high β-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals

Enhanced high β-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals

First successful head‐start program of leatherback sea turtles (Dermochelys coriacea) in Thailand and proposed dietary strategy

Physical Pretreatments of Cassava Chips Influenced Chemical Composition, Physicochemical Properties, and In Vitro Digestibility in Animal Models

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