Partial purification and characterization of an endonuclease from spinach that cleaves ultraviolet light-damaged duplex DNA

Doetsch, Paul W.; McCray, William; Valenzuela, Mario R.L.

doi:10.1016/0167-4781(89)90153-x

Cited by 20 publications

(17 citation statements)

References 16 publications

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“…Moreover, it did not act on single-stranded nucleic acids [235]. Single-strandspeci¢c P1 [236], spinach [78] and avena leaf [41] nucleases preferentially attack adenylic acid linkages in DNA. Moreover P1 nuclease preferred 3P-ribonucleotide monophosphates to 3P-deoxyribonucleotide monophosphates [185].…”

Section: Base/linkage Speci¢citymentioning

confidence: 99%

Single-strand-specific nucleases

Desai

2003

FEMS Microbiology Reviews

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Single-strand-specific nucleases are multifunctional enzymes and widespread in distribution. Their ability to act selectively on singlestranded nucleic acids and single-stranded regions in double-stranded nucleic acids has led to their extensive application as probes for the structural determination of nucleic acids. Intracellularly, they have been implicated in recombination, repair and replication, whereas extracellular enzymes have a role in nutrition. Although more than 30 single-strand-specific nucleases from various sources have been isolated till now, only a few enzymes (S1 nuclease from Aspergillus oryzae, P1 nuclease from Penicillium citrinum and nucleases from Alteromonas espejiana, Neurospora crassa, Ustilago maydis and mung bean) have been characterized to a significant extent. Recently, some of these enzymes have been cloned, their crystal structures solved and their interactions with different substrates have been established. The detection, purification, characteristics, structure^function correlations, biological role and applications of single-strand-specific nucleases are reviewed.

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Section: Base/linkage Speci¢citymentioning

confidence: 99%

Single-strand-specific nucleases

Desai

2003

FEMS Microbiology Reviews

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“…Although developed initially as a qualitative technique, it can be augmented and used as a quantitative method by end-labelling the substrate [69] or by densitometric scanning following electrophoresis in agarose gels [70]. A unit of the enzyme is de¢ned as the amount of enzyme required to produce 1 fmol of nicks in the plasmid DNA under the assay conditions.…”

Section: Gel Electrophoresismentioning

confidence: 99%

Sugar non-specific endonucleases

Rangarajan¹

2001

FEMS Microbiology Reviews

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Sugar non-specific endonucleases are multifunctional enzymes and are widespread in distribution. Apart from nutrition, they have also been implicated in cellular functions like replication, recombination and repair. Their ability to recognize different DNA structures has also been exploited for the determination of nucleic acid structure. Although more than 30 non-specific endonucleases have been isolated to date, very little information is available regarding their structure^function correlations except that of staphylococcal and Serratia nucleases. However, during the past few years, the primary structure, nature of the active site based on sequence homology, and the probable mechanism of action have been postulated for some of the enzymes. This review describes the purification, characteristics, biological role and applications of sugar non-specific endonucleases. ß

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“…In addition to avoidance of UV damage by means of shielding pigments, DNA repair also plays an essential role in UV protection. In higher plants two ways of DNA repair are known so far: excision repair, which includes endonucleolytic steps, base removal, and DNA re-synthesis (McLennan, 1987;Doetsch et al, 1989;Britt et al, 1993) and photorepair by PRE (EC 4.1.99.3). PRE monomerizes CPD in a light-dependent reaction (for review, see Sancar, 1994).…”

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confidence: 99%

Ultraviolet Light Inhibition of Phytochrome-Induced Flavonoid Biosynthesis and DNA Photolyase Formation in Mustard Cotyledons (Sinapis alba L.)

1995

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I n cotyledons of etiolated mustard (Sinapis a b a L.) seedlings, phytochrome-far-red-absorbing forminduced flavonoid biosynthesis was found to be inhibited by short-term ultraviolet .(UV) irradiations. UV inhibition was shown for the synthesis of quercetin, anthocyanin, and also for the accumulation of the mRNA for chalcone synthase, the key enzyme of this pathway. The UV effect was more pronounced on flavonoid biosynthesis, a process that selectively occurs i n the epidermal layers, than on the synthesis of mRNA for chlorophyll a/b-binding protein localized in the mesophyll tissue. These UV inhibitory effects were accompanied by cyclobutane pyrimidine dimer (CPD) formation showing a linear fluence-response relationship. CPD formation and UV inhibition of flavonoid biosynthesis was found to be partially reversible by blue/UV-A light via DNA photolyase (PRE), allowing photoreactivation of the DNA by splitting of CPDs, which are the cause of the UV effect. Like flavonoid formation PRE was also induced by the far-red-absorbing form of phytochrome and induction was inhibited by UV. A potentia1 risk of inhibition, in response to solar UV-B irradiation, was shown for anthocyanin formation. This inhibition, however, occurred only if photoreactivation was experimentally reduced. The PRE activity present in the etiolated seedlings (further increasing about 5-fold during light acclimatization) appears to be sufficient to prevent the persistence of CPDs even under conditions of high solar irradiation.

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Partial purification and characterization of an endonuclease from spinach that cleaves ultraviolet light-damaged duplex DNA

Cited by 20 publications

References 16 publications

Single-strand-specific nucleases

Single-strand-specific nucleases

Sugar non-specific endonucleases

Ultraviolet Light Inhibition of Phytochrome-Induced Flavonoid Biosynthesis and DNA Photolyase Formation in Mustard Cotyledons (Sinapis alba L.)

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