Partial purification and characterization of a cellular protein that binds to the SV40 core origin of DNA replication

Alliger, Peter; Traut, W; Carstens, Eric B.; Fanning, Ellen

doi:10.1016/0167-4781(88)90112-1

Cited by 7 publications

(2 citation statements)

References 16 publications

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“…These activities might be related to other cellular proteins binding the SV40 core origin that have been reported by others (Baur & Knippers, 1988;Alliger et al, 1988;Gaillard et al, 1988;Jones et al, 1988;Malkas & Baril, 1989;Galli et al, 1992;Carmichael et al, 1993). The competition experiments reported here indicate that the sequence-specific binding proteins interact with sites that are present in the late region of the origin.…”

Section: Discussionsupporting

confidence: 52%

Cruciform DNA binding protein in HeLa cell extracts

Pearson

Ruiz²,

Price³

et al. 1994

Biochemistry

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We have analyzed by band-shift assays HeLa cell protein-DNA interactions on a stable cruciform DNA molecule. The stable cruciform was formed by heteroduplexing the HindIII-SphI fragment of SV40 virus DNA that contains the origin of replication with a derivative mutant containing a heterologous substitution at the central inverted repeat. We have identified a novel binding activity in HeLa cell extracts with specificity for the cruciform-containing DNA and no apparent sequence specificity. The activity is protein-dependent, void of detectable nuclease activity, and distinct from that reported for HMG1. A cruciform binding protein (CBP) with an apparent molecular weight of 66 kDa was enriched from HeLa cell extracts. In addition to the CBP, we have detected sequence-specific binding activities to sites proximal to the cruciform. Binding to one such site is increased in the cruciform-containing heteroduplex DNA by comparison to its linear homoduplex counterpart, suggesting transmission of structural effects by the stem-loops to their local environment.

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Section: Discussionsupporting

confidence: 52%

Cruciform DNA binding protein in HeLa cell extracts

Pearson

Ruiz²,

Price³

et al. 1994

Biochemistry

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“…Traut and Fanning (49) have identified a factor from primate cell lines which binds sequences at the SV40 origin of DNA replication, in both single-stranded and double-stranded DNA. The factor that they have identified binds a sequence conserved in a number of papovavirus origins and has a molecular weight of approximately 37,000 (1).…”

mentioning

confidence: 99%

The HeLa Pur factor binds single-stranded DNA at a specific element conserved in gene flanking regions and origins of DNA replication.

1992

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A major site of DNA bending is located 1.6 kb upstream of the P1 transcription start site of the human c-myc gene, near the center of a reported zone of initiation of DNA replication. A repeated, purine-rich element, termed PUR, at the bend site is specifically bound by a protein in HeLa cell nuclear extracts. This protein has specific affinity for the purine-rich single strand of the element. Methylation interference maps a pattern of specific contact points with guanosine bases in a 24-mer oligonucleotide containing the element. UV cross-linking reveals that contact is made by a polypeptide of approximately 28 kDa. The PUR element is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. The consensus sequence GGNNGAGGGAGARRRR has been derived. This element is present near centers of regions of two mammalian loci (human c-myc and hamster dhfr) recently reported as initiation zones for DNA replication. A 24-mer oligonucleotide representing the hamster dhfr version of the PUR element effectively competes with the human c-myc version for binding to Pur.It is not known at this time whether cis-acting elements serve to control the initiation of DNA replication in mammalian chromosomes. Furthermore, no mammalian protein has been shown to influence the initiation of replication through sequence-specific binding to DNA. It is, however, clear that initiation of replication in prokaryotes, lower eukaryotes, and mammalian DNA tumor viruses involves duplex opening mediated by the specific binding of an initiator protein to repeated DNA elements (for reviews, see references 6, 20, 24, and 48). Difficulty in identifying sequences controlling mammalian initiation has been due in part to a lack of suitable methods for mapping replication origins in large and complex genomes. Recent mapping procedures have improved the localization of initiation zones in the vicinity of certain mammalian genes, and at least two candidates for potential origins exist. Seven different mapping methods concur in locating an initiation zone approximately 17 kb 3' to the hamster dihydrofolate reductase

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Identification and purification of a protein that binds the yeast ARS consensus sequence

Hofmann¹,

Gasser²

1991

Cell

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Partial purification and characterization of a cellular protein that binds to the SV40 core origin of DNA replication

Cited by 7 publications

References 16 publications

Cruciform DNA binding protein in HeLa cell extracts

Cruciform DNA binding protein in HeLa cell extracts

The HeLa Pur factor binds single-stranded DNA at a specific element conserved in gene flanking regions and origins of DNA replication.

Identification and purification of a protein that binds the yeast ARS consensus sequence

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