Partial purification and characterization of a trypsin inhibitor isolated from Adenanthera pavonina L. seeds

Souza, Danilo Diego de; Costa, Romero Marcos Pedrosa Brandão; Albuquerque, Wendell; Porto, Ana Lúcia Figueiredo

doi:10.1016/j.sajb.2015.11.008

Cited by 14 publications

(7 citation statements)

References 33 publications

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“…Peptidase inhibitors, which include trypsin inhibitors, act as natural regulators of proteolytic enzymes 15 and are therefore targets for research with potential biotechnological applications in a number of areas, such as medicine and agriculture 16 . Trypsin inhibitors are often found in seeds, and many of these proteins have been purified and characterised 17 .…”

Section: Introductionmentioning

confidence: 99%

Biochemical characterisation of a Kunitz-type inhibitor fromTamarindus indicaL. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity

Medeiros

Costa

Carvalho

et al. 2018

Journal of Enzyme Inhibition and Medicinal Chemistry

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A trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in animals, increasing the cholecystokinin (CCK) in eutrophy and reducing leptin in obesity. We purified TTI (pTTI), characterised, and observed its effect upon CCK and leptin in obese Wistar rats. By HPLC, and after amplification of resolution, two protein fractions were observed: Fr1 and Fr2, with average mass of [M + 14H]+ = 19,594,690 Da and [M + 13H]+ = 19,578,266 Da, respectively. The protein fractions showed 54 and 53 amino acid residues with the same sequence. pTTI presented resistance to temperature and pH variations; IC50 was 2.7 × 10−10 mol.L−1 and Ki was 2.9 × 10−11 mol.L−1. The 2-DE revealed spots with isoelectric points between pH 5 and 6, and one near pH 8. pTTI action on leptin decrease was confirmed. We conclude that pTTI is a Kunitz trypsin inhibitor with possible biotechnological health-related application.

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Section: Introductionmentioning

confidence: 99%

Biochemical characterisation of a Kunitz-type inhibitor fromTamarindus indicaL. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity

Medeiros

Costa

Carvalho

et al. 2018

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…It was showed that trypsin inhibitor activity in soybeans was reduced of 26.3% during heattreatments [18]. Trypsin inhibitors from Leguminosae seeds are reported to be great thermo resistance to high temperatures [28]. The reduction or elimination of enzyme inhibitors of pulses could be made using different food processes, like dehulling, soaking, boiling, roasting, autoclaving, micronization, microwave cooking, extrusion cooking, fermentation and germination [20].…”

Section: Discussionmentioning

confidence: 99%

Influence of Different Cowpea (<i>Vigna unguiculata</i> (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition

Sombié

Coulibaly

Hilou

et al. 2019

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Cowpea is an important protein crop widespread in Africa. The purpose of this research was to determine the content of trypsin and chymotrypsin inhibitors in different genotypes of cowpea seeds. Trypsin percentage inhibition showed about 13.5-fold variation (5.12% ± 1.47 to 70.52% ± 6.58) between the cowpea genotypes respectively for KVx 421-2J and Kondèsyoungo local. The chymotrypsin inhibitory activity varies among cowpea genotypes from 21.19 to 76.94%. The highest percent inhibitory activity was exhibited by KVx 396-4-5-2D on chymotrypsin. This study also showed significant correlations between type of cowpea genotype and trypsin inhibition potential and also between colour and chymotrypsin inhibition potential of cowpea seeds. Kondèsyoungo local, a landraceae genotype exhibited the high potential to inhibit the trypsin enzyme. Coloured seeds of cowpea genotypes possess higher percentage inhibition of chymotrypsin than the colourless ones (p < 0.05). The calculated mean of trypsin and chymotrypsin inhibition activities showed that Labagela local genotype possess the highest inhibition activity of both protease enzymes. The PCA components analysis and the dendrogram performed basis on the protease inhibitory activities divided the thirty-one genotypes of cowpea used in this study into three classes. The results presented in this work can contribute greatly to the planning of a cowpea breeding program aimed at reducing the content of proteases inhibitors in order to improve the nutritional value of seeds or to increase PI content for tolerance to stored grain pests.

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“…Some work has reported the presence of proteins or peptides in A. pavonina seeds (Soares et al, 2012;Silva et al, 2012;Sasaki et al, 2015;Souza et al, 2016). Recently, Lavudi and Seshagirirao (2018) found an amount of protein in the crude seed extract of 82.11198 mg/ml, and a different protein profile was verified by an SDS-PAGE assay.…”

Section: Phytochemical Analysis Of Seedsmentioning

confidence: 97%

“…An interesting study evaluated the toxic potential of the purified trypsin from seeds of A. pavonina using A. salina lethality test. The results showed that a concentration of 0.16 mg/mL was enough to kill 100% of A. salina after 72 h (Souza et al, 2016).…”

Section: Toxicity Studiesmentioning

confidence: 98%

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Advances in the research of Adenanthera pavonina: From traditional use to intellectual property

GeronÃ§o¹,

Melo²,

Barros³

et al. 2020

J. Med. Plants Res.

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Adenanthera pavonina, an Asian native leguminous, is a well known highly used plant in traditional medicine. Such broad herbal medicine applications motivated several researchers to study its chemical composition, mainly of its leaves, seeds, and barks, besides the pharmacological effects aiming to demonstrate scientific evidence of its empirical uses. Most studies have been conducted with plant derivatives, and more recent studies have focused on isolated compounds. The phytochemical screening revealed various secondary metabolites with diverse biological effects, including flavonoids, alkaloids, saponins, tannins, steroids, triterpenoids, polyphenols, anthraquinones, coumarins, glycosides and polysaccharides. Experimental studies of this plant have shown numerous pharmacological activities such as antidiarrheal, anti-inflammatory, antinociceptive, antioxidant, antimicrobial, anticancer, and others. Advances in the studies of phytochemistry and biological activity on this plant species over the last years led to the increasing interest of researchers in the protection of inventions focusing on its cosmetic, pharmaceutical and food applicability. This review summarizes the tremendous therapeutic and technological potential of A. pavonina that should be explored, opening new perspectives for future researches and the development of new products.

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Partial purification and characterization of a trypsin inhibitor isolated from Adenanthera pavonina L. seeds

Cited by 14 publications

References 33 publications

Biochemical characterisation of a Kunitz-type inhibitor fromTamarindus indicaL. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity

Biochemical characterisation of a Kunitz-type inhibitor fromTamarindus indicaL. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity

Influence of Different Cowpea (<i>Vigna unguiculata</i> (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition

Advances in the research of Adenanthera pavonina: From traditional use to intellectual property

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Partial purification and characterization of a trypsin inhibitor isolated from Adenanthera pavonina L. seeds

Cited by 14 publications

References 33 publications

Biochemical characterisation of a Kunitz-type inhibitor fromTamarindus indicaL. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity

Biochemical characterisation of a Kunitz-type inhibitor fromTamarindus indicaL. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity

Influence of Different Cowpea (&lt;i&gt;Vigna unguiculata&lt;/i&gt; (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition

Advances in the research of Adenanthera pavonina: From traditional use to intellectual property

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Influence of Different Cowpea (<i>Vigna unguiculata</i> (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition