Partial Purification and Properties of Adenosine Triphosphatase (ATPase) from Liver Fluke Fasciola hepatica

Abstract

Objective: The adenosine triphosphatase (ATP phosphohydrolase, EC 3.6.1.3.;ATPase) is a membrane -bound enzyme which transport protons across the plasma membrane using ATP as an energy source. Methods: The adenosine triphosphatase (ATPase ; EC: 3.6.1.3) was extracted from membrane preparations of adult Fasciola hepatica by chloroform treatment and purified by means of ammonium sulphate fractionation, gel filtration on sephadex G-200 and DEAE-Cellulose chromatography. Results: The molecular weight was calculated to be 305.000 dalton by gel filtration. Kinetic experiments demonstrated a biphasic linear lineweaver -burk relationship (km=0.142 and 1.66 mM) thus revealing the existence of two substrate binding enzyme sites. Conclusion: In our study revealed that partial inhibition of Mg 2+ dependent purified enzyme by oligomycin suggest the absence of mitochondrial ATPase in F. hepatica. (Turkiye Parazitol Derg 2014; 38: 26-31)

Tera-scale astronomical data analysis and visualization

“…Previously, we have developed a distributed GPU-based framework for volume rendering large data and Hassan et al (2012)]. In this work, we generalise this framework beyond qualitative volume rendering.…”

“…It is the addition of the peer-to-peer processing mode that enables an efficient implementation of volume rendering with a generic transfer function, compared to our earlier solution, which used maximum intensity projection transfer function and Hassan et al (2012)].…”

“…The usage of the maximum intensity projection (MIP) has been addressed by us before in (Hassan et al , 2012. The main advantage of using MIP as transfer function is its simplicity from the user perspective.…”

“…Within this work, we re-implement this method using the peer-to-peer processing mode to put it in a unified implementation framework with the generic transfer function's volume rendering method [see Section 3.4 for details]. While the peer-to-peer processing mode increases the overall rendering time [with respect to the results published in Hassan et al ( , 2012], it is still within the interactive limit (which we define to be higher than five frames per second).…”

“…Within Hassan et al ( , 2012 we discussed a direct send approach to implement distributed volume rendering of larger than memory datasets. While the framework introduced in these papers can still be extended to support other types of transfer functions, it might not produce the best frame rate.…”