Partial Purification of a Water‐Extractable Rye (Secale cereale) Protein Capable of Improving the Quality of Wheat Bread

Abstract

Rye water‐soluble extracts contain a protein fraction that, when added at low concentrations to a straight‐dough breadmaking recipe, significantly increased bread volume. Enrichment of the active component is possible by anion‐exchange fractionation with diethylaminoethyl‐cellulose (DEAE), by ammonium sulfate precipitation, or by using rye bran or shorts milling fractions as the starting material. The active material was not bound to DEAE‐cellulose. With ammonium sulfate precipitation, the fractions obtained at 30, 40, and 50% saturation were active in straightdough baking experiments. Iso‐electric focusing revealed that fractions active in breadmaking invariably contained alkaline protein fractions (pI > 7.5). Inactivation of enzyme material by boiling the water‐soluble extract from rye destroyed all breadmaking activity. The activity of the bread improver was additive to that of potassium bromate but not to that of ascorbic acid. It was not counteracted by catalase, showing that it does not work by a mechanism involving the production of hydrogen peroxide. The extract was not able to overcome the detrimental effect on bread quality resulting from mixing dough in a nitrogen atmosphere.

Studies on Rye (Secale cereale L.) Lines Exhibiting a Range of Extract Viscosities. 2. Rheological and Baking Characteristics of Rye and Rye/Wheat Blends and Feeding Value for Chicks of Wholemeals and Breads

“…Weipert et al () reported that flours from rye cultivars exhibiting high total arabinoxylan content and a high proportion of WEAX yielded rye breads (using a sourdough formula) of good quality. Similarly, including WEAX from rye in a wheat bread formulation improved baking quality ( − ). On the basis of these studies, it could be concluded that the concentration of WEAX is an important factor determining the baking quality of rye and that higher levels of WEAX would be associated with superior baking quality.…”