Partial purification of an antifungal protein produced byEnterococcus faecalis CHD 28.3

Roy, Utpal; Kaushik, Jai K.; Grover, Sunita; Batish, V. K.

doi:10.1007/bf03178329

Cited by 7 publications

(6 citation statements)

References 30 publications

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“…The impact was concentrationdependent, as reflected by the inhibition zone diameters and CFS from E. faecalis isolated from Cheddar cheese (Ch9) have the highest effect. This is in context with Roy et al [25] who have shown that E. faecalis isolated from Cheddar cheese produce effective antifungal protein. These data are in agreement with others [11,18,25,26] who found that the identified E. faecalis non-haemolytic anticandida protein (ACP), might be developed as an effective treatment for candidiasis and its complications especially in immunocompromised individuals.…”

Section: Resultssupporting

confidence: 76%

Enterococcus faecalis Cell-Free Supernatant Inhibits Hyphal Morphogenesis and Biofilm Formation in Candida albicans

Hassan

Tartor

Ashour

et al. 2018

Zagazig Veterinary Journal

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Enterococcus faecalis and Candida albicans are two of the most significant opportunistic and intricate nosocomial pathogens. They reside overlapping niches as major constituents of the gastrointestinal (GI) and oral microbiome. This study was designed to investigate the interaction between these two opportunistic pathogens that could affect treatment strategies and influence the interkingdom signaling and sensing in the microbiome conceptions. E. faecalis was isolated from 150 samples collected from human urine, cattle raw milk, milk products and chicken intestine and identified by both the routine microbiological and molecular identification methods based on 16S rRNA gene. Isolates susceptibility to nine antimicrobials was analyzed using the disk-diffusion method and E. faecalis isolates showed multidrug resistance. Most of C. albicans isolates were able to form biofilm with different intensities. Cell-free supernatant (CFS) of E. faecalis was purified and tested against C. albicans and/or biofilm formation. It inhibited the growth of C. albicans and their filamentation with regards to biofilm formation in vitro. The data presented here demonstrated the potential antifungal activity of E. faecalis CFS and could explain the biological interaction between these two microbes in one hand and the possibility of using E. faecalis CFS as a biological product to control yeast infection in the other hand.

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Section: Resultssupporting

confidence: 76%

Enterococcus faecalis Cell-Free Supernatant Inhibits Hyphal Morphogenesis and Biofilm Formation in Candida albicans

Hassan

Tartor

Ashour

et al. 2018

Zagazig Veterinary Journal

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“…Enterococcus spp. are rarely described to antifungal activities [12][13][14] 11 was reported to inhibit the growth of Fusarium culmorum and P. expansum [14].…”

Section: Resultsmentioning

confidence: 99%

Screening of Lactic Acid Bacteria for Antifungal Activity against Fungi

Kıvanç

Kıvanç²,

Pektas³

2014

J Food Process Technol

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Many chemical preservatives that target fungi growth in food have been approved and used for many years. Recently the consumers are looking and demanding for products without chemical preservatives and still maintain good shelf life and safe. The growth of spoilage fungi have been a global concern because of the economy loses and the health hazard of the mycotoxins produced by the spoilage fungi. A total of 22 lactic acid bacteria isolated from Tarhana and Lactic acid bacteria were screened for antifungal activity using dual agar overlay method and well method against Alternaria alternata, Aspergillus parasiticus, Aspergillus oryzae Penicillium griseofulvum, Penicillium chrysogenum, Penicillium notatu, Penicillium citrinum, Penicillium roquefort, Aspergillus fumigatus. Ten isolates showed inhibition activity after 72 h incubation at 30°C. Supernatant of 10 isolates with strong antifungal activity was evaluated by well method and they inhibited the growth of the fungi at 30°C for 72 h. F2,1 supernatant reduced the mass growth of Penicillium griseofulvum, Penicillium chrysogenum Aspergillus fumigatus and Aspergillus parasiticus when incubated for 6 days at 30°C. The isolates were identified using rapid ID 32 Strep as Enterococcus durans F2.1. F2.1 isolates studied inhibited the growth of the mycelia and conidia germination of the fungi which indicate the possibility of using LAB isolates as biopreservative.

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“…LAB strains belonging to genus Enterococcus are rarely described to harbour antifungal activities (Roy et al . ; Shekh et al . ).…”

Section: Resultsmentioning

confidence: 99%

“…These reactions on yeast's nucleus and vacuoles were observed and described also when yeast cells are submitted to strong stress by sonication or by heating (Webster and Watson 1993;Radel et al 2000). LAB strains belonging to genus Enterococcus are rarely described to harbour antifungal activities (Roy et al 2009;Shekh et al 2011). Enterococcus durans A5-11 was described as bacteriocinogenic and, as demonstrated in this article, has antagonistic effects against various fungal strains, including D. hansenii LMSA2.11.003, F. culmorum LMSA1.12.136, Candida krusei LMSA2.12.020, P. expansum LMSA1.08.102 and Penicillium roqueforti LMSA1.12.138.…”

Section: Changes Of Cell Ultrastructures Induced By Treatments With Cmentioning

confidence: 98%

Antifungal properties of durancins isolated from Enterococcus durans A5-11 and of its synthetic fragments

Belguesmia

Choiset

Rabesona

et al. 2013

Lett Appl Microbiol

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Significance and Impact of the Study: Antifungal properties of durancins isolated from Enterococcus durans A5-11 and of their chemically synthesized fragments were determined. Treatment of D. hansenii LMSA2.11.003 strain with 2 mmol l À1 of the synthetic peptides led to the loss of the membrane integrity and to several changes in the ultra-structure of the yeast cells. This work contributes to improve understanding of molecular causes of antimicrobial activities of bacteriocins and their fragments. It may be proposed that the studied peptides affect all the yeast cellular and intramembranes including cytoplasmatic reticulum and nuclear and vacuolar membranes. AbstractThe aim of this work was to study the antifungal properties of durancins isolated from Enterococcus durans A5-11 and of their chemically synthesized fragments. Enterococcus durans A5-11 is a lactic acid bacteria strain isolated from traditional Mongolian airag cheese. This strain inhibits the growth of several fungi including Fusarium culmorum, Penicillium roqueforti and Debaryomyces hansenii. It produces two bacteriocins: durancin A5-11a and durancin A5-11b, which have similar antimicrobial properties. The whole durancins A5-11a and A5-11b, as well as their N-and C-terminal fragments were synthesized, and their antifungal properties were studied. C-terminal fragments of both durancins showed stronger antifungal activities than other tested peptides. Treatment of D. hansenii LMSA2.11.003 strain with 2 mmol l À1 of the synthetic peptides led to the loss of the membrane integrity and to several changes in the ultrastructure of the yeast cells. Chemically synthesized durancins and their synthetic fragments showed different antimicrobial properties from each other. N-terminal peptides show activities against both bacterial and fungal strains tested. C-terminal peptides have specific activities against tested fungal strain and do not show antibacterial activity. However, the C-terminal fragment enhances the activity of the N-terminal fragment in the whole bacteriocins against bacteria.

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Partial purification of an antifungal protein produced byEnterococcus faecalis CHD 28.3

Cited by 7 publications

References 30 publications

Enterococcus faecalis Cell-Free Supernatant Inhibits Hyphal Morphogenesis and Biofilm Formation in Candida albicans

Enterococcus faecalis Cell-Free Supernatant Inhibits Hyphal Morphogenesis and Biofilm Formation in Candida albicans

Screening of Lactic Acid Bacteria for Antifungal Activity against Fungi

Antifungal properties of durancins isolated from Enterococcus durans A5-11 and of its synthetic fragments

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