Partial purification of invertase from Momordica charantia (bitter melon) by three phase partitioning (TPP) method

BelligÃ1⁄4n, Nihan KÃ1⁄4bra; Demir, Burcu SaygÄ±deÄŸer

doi:10.5897/ajbr2019.1033

Cited by 6 publications

(2 citation statements)

References 33 publications

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“…The studies reported on the TPP method used for concentration and purification of proteases from Cucumismelo (Cucumisin) and Calotropis procera have also demonstrated high purification fold and activity recovery at 60%–65% salt saturation which is close to our findings in the present study (Gagaoua & Hafid, 2016; Rawdkuen et al, 2010). The study by Nihan et al on invertase extraction from bitter melon using the TPP method has also reported the highest purification fold and enzyme recovery at 70% (NH₄)₂SO₄ saturation (Nihan & Burku, 2019).…”

Section: Resultsmentioning

confidence: 99%

Three‐phase partitioning as an efficient one‐step method for the extraction and purification of bromelain from pineapple crown waste

Gul

Khan

Arain

et al. 2022

Food Processing Preservation

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Bromelain is a protease that has multiple applications in food industry. This study focused on the partial purification of bromelain from pineapple crown using the three‐phase partitioning (TPP) method. Different parameters of TPP (ratio of crude extract to t‐butanol (v/v), percentage of (NH4)2SO4, and pH) were optimized for the concentration and purification of bromelain. The optimized parameters of the TPP system, that is, 1.0:0.5 crude extract to t‐butanol ratio at pH 7.0 with 70% (NH4)2SO4 saturation, gave significantly high purification fold of 3.4 (p‐value ≤.000) with a significant increase in percent enzyme activity recovery of 244% (p‐value ≤.000). The purified enzyme was predominantly partitioned at the interfacial phase and the molecular weight of bromelain was found to be around 26 kDa. Characterization of bromelain demonstrated the highest relative activity at pH 7.0 and 35°C. This study showed the effective and economical process development of bromelain purification from pineapple waste at laboratory scale using the TPP method. Novelty impact statement The current study reported the purification of industrially important enzyme Bromelain using three‐phase partitioning (TPP) method, which has not been previously studied. TPP method was effectively used as a single, scalable, non‐chromatographic method for bromelain purification from crown waste of pineapple. The optimized parameters (70% (NH4)2SO4 saturation with 1.0:0.5 ratio of crude extract to t‐butanol at pH 7.0), resulted in the purification fold of 3.4 with a recovery of 244%.

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Section: Resultsmentioning

confidence: 99%

Three‐phase partitioning as an efficient one‐step method for the extraction and purification of bromelain from pineapple crown waste

Gul

Khan

Arain

et al. 2022

Food Processing Preservation

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“…Optimal conditions that resulted in higher invertase recovery in the purification procedure. (Nihan kubra Belligun and Burcu Saygideger Demir et al, 2019) [24] Invertase enzyme in the presence of surfactants is the next approach. This autolyzate was combined with 0.12 sodium picrate at a 1:0.35 ratio.…”

Section: A Nigermentioning

confidence: 99%

Invertase enzyme

Siddiqa¹,

Amin²

2022

Int. J. Adv. Biochem. Res.

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The enzyme known as invertase catalysis the conversion of sucrose into glucose and fructose (EC 3.2.1.26). The best source of invertase is yeast. Internal and exterior in vertases are the two varieties found in S. cerevisiae. Invertase catalyses the hydrolysis of sucrose into fructose and glucose. Pharmaceuticals, analytical measuring probes, and yeast species detection ELISA kits all include this invertase. Immobilization of the invertase naturally is what is meant by this. Different levels of glycoprotein polymerization, glycosylation, and phosphorylation make bacteria invertase unique. A range of gram-positive and gram-negative organisms, including Zymomonas mobilis, and Bacillus strains, produce invertase. Comparing fungal growth to the time course of invertase synthesis by fungi, which ranged from 24 to 192 hours, was analysed. Twenty-four-hour intervals were used to measure the invertase activity. The biological characteristics that were looked at in this study included the amount of invertase-producing bacteria and soil invertase activity. Invertase is used to replace traditional acid hydrolysis.

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Three-phase partitioning for the separation of proteins, enzymes, biopolymers, oils and pigments: a review

et al. 2022

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Partial purification of invertase from Momordica charantia (bitter melon) by three phase partitioning (TPP) method

Cited by 6 publications

References 33 publications

Three‐phase partitioning as an efficient one‐step method for the extraction and purification of bromelain from pineapple crown waste

Three‐phase partitioning as an efficient one‐step method for the extraction and purification of bromelain from pineapple crown waste

Invertase enzyme

Three-phase partitioning for the separation of proteins, enzymes, biopolymers, oils and pigments: a review

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