Partial Purification of the Ovarian Oviposition-Inducing Factor and Estimation of its Chemical Nature

Abstract

The ovarian ruptured follicles larger than 0.2 g. of wet weight were collected from laying hens and an ovarian oviposition-inducing factor (OOIF) was extracted from the follicles. The extract was chromatographed on a column of Sephadex G-75. Material from the active region for inducing premature oviposition was subjected to thioglycollate or trypsin treatment. It was found that oxytocic activity of this material was completely destroyed by thioglycollate buy not by trypsin, when tested on the contractility of the isolated hen's uterus. The OOIF was found to be diffusible through a cellophane bag. It is suggested that the OOIF may be a relatively small molecule have a disulfied bridge and it seems to be a hormone or hormone-like substance resembling the posterior pituitary hormones in the chemical nature.

Changes in the plasma concentration of immunoreactive arginine vasotocin during oviposition in the domestic fowl

“…On the other hand, the ruptured ovarian follicle has been reported to have a role in controlling the timing of oviposition (Rothchild and Fraps, 1944). An ovarian oviposition-inducing factor, which causes premature oviposition but is neither a prostaglandin nor a steroid hormone, has been found in the ruptured follicles (Tanaka and Goto, 1976). This factor is present in high concentrations in the largest follicle occurring near the time of oviposition (Tanaka, 19766).…”

Histophysiological Studies on the Postovulatory Follicles of the Fowl Ovary

“…Tanaka and Nakada (1974) demonstrated that the fowl postovulatory follicles secrete an unknown nonlipoidal component, probably the oxytocin (Soliman and Walker, 1975), which is involved in genital tract motility and oviposition. Tanaka and Goto (1976) have denied the secretion of oxytocic substance. The thecal gland cells persist for a longer period and may secrete either progesterone (Chieffi and Botte, 1965) or oestrogens (Guraya, 1976a).…”

Studies on the contractility of follicular wall with special reference to the mechanism of ovulation in hens

“…Substances were obtained from the following suppliers: PGE 2 , PGF 2a (Ono Pharmaceutical Co., Japan) ; histamine, adrenaline and noradrenaline (Sigma); oxytocin (Teikokuzoki Pharmaceutical Co., Japan); acetylcholine (Yoneyama Pharmaceutical Co., Japan); atropine sulphate (Merck); dibenamine (Tokyo Kasei Co., Japan); propranolol (Sumitomo Chemical Co., Japan). OOIF (ovarian oviposition inducing factor) was obtained by the method of Tanaka and Goto (1976).…”