Partial Sequencing and Identification of Three Proteins from Equine Uterine Secretion Regulated by Progesterone

Beier-Hellwig, K.; Kremer, H; Bonn, B.; Linder, Dietmar; Bader, Hermann; Beier, H. M.

doi:10.1111/j.1439-0531.1995.tb00627.x

Cited by 19 publications

(20 citation statements)

References 13 publications

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“…Nevertheless, the apical intracellular distribution and the positively stained material in the lumina of the glands, both detectable during interestrus, possibly correspond to a release of proteins from secretory vesicles of uterine glands. These results supporting the hypothesis that the synthesis and/or secretion of UGL is influenced by estrogen (Beier-Hellwig et al 1995).…”

Section: Discussionsupporting

confidence: 80%

“…These differences may reflect individual differences in serum hormone profiles, as described for the (Kikukawa et al 1988). In contrast to uterine secretions which showed highest levels of UGL during interestrus (Beier-Hellwig et al 1995), a maximal peak of intracellular UGL were detectable at estrus by immunohistochemistry. However it remains unclear if this accumulated UGL is being released into the uterine lumen or if it reflects an estrogen induced intraepithelial accumulation (Beier 2000).…”

Section: Discussionmentioning

confidence: 87%

“…A significant increase in macromolecules, predominantly proteins, characterize histotroph milieu during the preimplantation stage of uterine "receptivity", that facilitates implantation (Beier-Hellwig et al 1995). However, in species like the horse, in which the trophoblast is non-invasive, uterine secretions are more likely to play an important role in development and survival of the conceptus (Amoroso 1959) and are more abundant due to accumulation of endometrial secretory proteins during the luteal phase as compared to other mammalian species (Beier-Hellwig et al 1995).…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical and histochemical identification of proteins and carbohydrates in the equine endometrium: Reaction patterns in the cycling mare

Hoffmann¹,

Bazer²,

Klug³

et al. 2009

PHK

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immunohistochemical and histochemical identification of proteins and carbohydrates in the equine endometrium: Reaction patterns in the cycling mare

Hoffmann¹,

Bazer²,

Klug³

et al. 2009

PHK

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“…P19 is produced in large quantities by the endometrium of the mare during the luteal phase of the estrous cycle and early pregnancy, and its secretion can be induced by the administration of exogenous progesterone ( [4,6]; J. Hyland, personal communication). In addition, it is very likely that the endometrial protein ''U1'' described by Zavy et al in 1982 [3], and later shown to be progesterone dependent by Hinrichs and Kenny [7], was P19.…”

Section: Introductionmentioning

confidence: 99%

Transfer of a Uterine Lipocalin from the Endometrium of the Mare to the Developing Equine Conceptus1

Crossett

Suire

Herrler

et al. 1998

Biology of Reproduction

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One of the major, progesterone-dependent proteins secreted into the uterine lumen of the mare is a 19-kDa lipocalin (P19). It associates strongly with the embryonic capsule that envelops the young horse conceptus in early gestation, suggesting that it may be involved in sustaining early development. However, it was not known whether the protein was transported through the capsule and/or trophoblast layer and into the yolk sac cavity. To address this question, polyclonal antisera were raised against a C-terminal peptide (based on the deduced amino acid sequence of P19) and a recombinant-derived P19 fusion protein. The antiserum raised against the C-terminal peptide recognized P19 on Western blots of denatured uterine secretions (subjected to SDS-PAGE), but it did not bind to the protein in tissue sections. However, the antiserum raised against the recombinant-derived fusion protein recognized P19 both on Western blots and in histological sections. Western blot analysis of tissues and fluids collected from early-pregnant mares demonstrated significant quantities of P19 in the endometrium and uterine secretions and in the embryonic capsule, the chorion, and the yolk sac fluid, showing that the protein is transferred through to the developing embryo. Concentrations of immunoreactive P19 declined during gestation so that, by Day 30, it had virtually disappeared from both maternal and fetal tissues and fluids. Immunohistochemical staining of endometrial biopsies collected during early pregnancy localized P19 to the glandular and luminal epithelia and to the lumina of the endometrial glands. The capsule and the trophoblast layer of the chorion from early (Days 16-17) horse conceptuses also stained positively with localization of P19 to the apical surface of the trophoblast cells. There was no detectable staining either in or on the embryonic disc. The presence of P19 in both the trophoblast layer and the yolk sac fluid suggests that P19 passes into the yolk sac fluid via trophoblast cells.

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“…The lipocalin uterocalin (UC) (Beier-Hellwig et al 1995), uteroglobin (UG), which serves as an anti-inflammatory agent and immune-modulator (Miele et al 1987, Mukherjee et al 1999, and the iron-binding protein uteroferrin (UF) (Bazer et al 1975), belong to a wide group of endometrial secretory proteins, whereas calbindin (CAL) is part of a family of intracellular calcium-binding proteins (Wooding et al 1996). …”

Section: Introductionmentioning

confidence: 99%

Histopathological and immunohistochemical findings during the sexual cycle in female mules and effects of long-term Regumate® administration

Jäger¹,

Schoon²,

Ellenberger³

et al. 2008

PHK

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SummaryThe aim of the first part of this study was a comparative immunohistochemical characterisation of the uterine secretory proteins uteroglobin (UG), uterocalin (UC), uteroferrin (UF) and of the calcium-binding protein calbindin (CAL) in the endometrium of clinically acyclic (n = 4) and "cyclic" mules (n = 2) considering clinical-gynaecological findings, endometrial functional morphology and blood serum concentrations of progesterone and estradiol. For this purpose a clinical-gynaecological examination was performed in five animals from April to August and in one mule until November 2004. Endometrial biopsies were taken concurrently (acyclic mules n = 46 biopsies, "cyclic" mules n = 51 biopsies). The endometrial proteins UG, UC, UF and CAL showed a very slight expression in the inactively differentiated endometria of the acyclic mules. In the endometria of the "cyclic" mules the secretion of these proteins was highly variable and no correlation between the phases of the sexual cycle, the concentration of the serum hormone values or the endometrial functional morphology could be detected. In the second part of the study two of the acyclic mules received an oral dose of 0.044 mg/kg BW (body weight) Regumate ® for 50 days in order to examine the influence of a long-term progestin administration on the endometrial functional morphology. During the treatment period until 27 days post administration the mules were weekly biopsied and examined both gynaecologically and endocrinologically. Two years after the treatment endometrial biopsies were collected once more. The results obtained prior to Regumate ® administration were compared with those during and after the treatment. On that account, 28 endometrial biopsies were examined pathohistologically (H. E. staining) and imunohistologically (UG, UC, UF, CAL, estrogen receptor (ER), progesterone receptor (PR), Ki-67 antigen). Prior to Regumate ® medication the endometrium of the acyclic mule mares exhibited an irregular inactive differentiation with a very faint immunolabelling of all uterine proteins investigated. During the medication the endometrium developed a highly irregular secretory differentiation associated with a distinctly increased secretion of CAL, while the secretion of the other investigated proteins remained low. After cessation of the Regumate ® administration as well as two years later the endometrium was found to be similarly irregular and inactive as before the treatment, the secretion pattern of the endometrial proteins was comparable to that before medication. In the course of the treatment an overall increased and highly variable expression of the steroid hormone receptors was noted. The administration of Regumate ® did not show any significant influence on the endometrial proliferative activity. The results of this study clearly show that in mule mares an exogenous long-term administration of progestins causes an exceedingly pronounced secretory differentiation in the endometrium of acyclic mules, and beyond this generates distinctly visible a...

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Partial Sequencing and Identification of Three Proteins from Equine Uterine Secretion Regulated by Progesterone

Cited by 19 publications

References 13 publications

Immunohistochemical and histochemical identification of proteins and carbohydrates in the equine endometrium: Reaction patterns in the cycling mare

Immunohistochemical and histochemical identification of proteins and carbohydrates in the equine endometrium: Reaction patterns in the cycling mare

Transfer of a Uterine Lipocalin from the Endometrium of the Mare to the Developing Equine Conceptus1

Histopathological and immunohistochemical findings during the sexual cycle in female mules and effects of long-term Regumate® administration

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