Partial Substitution of Glucose with Xylitol Prolongs Survival and Suppresses Cell Proliferation and Glycolysis of Mice Bearing Orthotopic Xenograft of Oral Cancer

Sahasakul, Yuraporn; Angkhasirisap, Wannee; Lam‐ubol, Aroonwan; Aursalung, Amornrat; Sano, Daisuke; Takada, Kazuya; Trachootham, Dunyaporn

doi:10.3390/nu14102023

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…Xylitol helps control blood sugar and obesity, facilitates wound healing, improves skin condition, reduces bone resorption, increases bone strength, and reduces ear and respiratory infections [41][42][43][44][45][46]. Xylitol also inhibits tumor growth in several studied cancer cell lines [47][48][49].…”

Section: Introductionmentioning

confidence: 99%

Salivary Transcriptome and Mitochondrial Analysis of ASD Children Compared to Healthy Controls

Cannon,

Toma,

Ganeshan

et al. 2024

Preprint

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Autism has been described as a gastrointestinal and neurological condition that is represented by impairments in language and social situations. Recently reported increases in the occurrence of Autism Spectrum Disorder (ASD) have resulted in increased research into the environmental differences between areas with a high occurrence, as in the “Western” diet populations, and a more locally derived diet population, wherein the rate appears to be significantly less. Thirty autistic children (USA) ages 6 to 21, were sampled at three different intervals: prior to any intervention, post-xylitol intervention, and post-probiotic intervention. The sampling consisted of buccal swabs for MITOSWAB testing, and saliva for metatranscriptomics to determine the entire range of microorganisms and the metabolome. In addition, a “Blue Zone” subject component that included 30 Colombian children, ages 6-16, and who were healthy and within normal behavior standards, also had buccal swabbing and salivary sampling performed for comparison along with 30 healthy (no history medications, illnesses, or behavior issues) USA children matched for age and gender. Saliva samples were analyzed metatranscriptomically and artificial intelligence was utilized to discern important differences in the microbiome that included bacteria, archaea, fungi, and viruses, and microbial functions. Significant differences were noted, and seen in the temporal data, demonstrating shifts that may have been precipitated by the interventions, with both the probiotics and the polyols. Particular bacterial strains were significantly more prevalent in the autism subject group including a strain that reduces neurotransmitters by enzymatic degradation. This provided supporting evidence to the hypothesis that the occurrence of autism may also be influenced by low Gamma Amino Butyric Acid (GABA) levels during development. Also, of interest was the un-expected finding that verbal skills dramatically increased in 6 of the 30 ASD subjects following xylitol supplementation.

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Section: Introductionmentioning

confidence: 99%

Salivary Transcriptome and Mitochondrial Analysis of ASD Children Compared to Healthy Controls

Cannon,

Toma,

Ganeshan

et al. 2024

Preprint

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“…Research demonstrates the inhibitory property of xylitol with many cancer cell lines when administered both dietary and systemically [20][21][22]. Because xylitol exhibits almost no side effects and is safely utilized by healthy human cells, it could be a beneficent natural supplement for potentially inhibiting cancer cell proliferation [23,24].…”

Section: Introductionmentioning

confidence: 99%

Effects of Xylitol on Tumor Progression in Syngeneic Mice Cancer Models

Cannon,

Cosantino,

Tesch

et al. 2024

Preprint

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This study investigates the effects of xylitol, a natural sugar alcohol, on tumor progression in syngeneic mouse cancer models. Xylitol has been known for its dental health benefits, but emerging evidence suggests broader biological roles, including potential anti-cancer properties. We explored xylitol's impact on two mouse cancer models: 4T1 mammary carcinoma and B16F10 melanoma. Xylitol's efficacy in inhibiting cancer cell lines and modulating tumor progression was assessed using immunocompetent female mice. The experiments involved intratumoral and peri-tumoral administration of a 20% xylitol solution in two mouse strains: BALB/c (4T1 mammary carcinoma) and C57BL/6 (B16F10 melanoma). Tumor volume, histopathology, and metabolomic analyses were conducted to gauge xylitol's influence. The study revealed that xylitol administration initially reduced tumor growth in the B16F10 melanoma model, accompanied by alterations in tumor metabolism. However, similar effects were not observed in the 4T1 mammary carcinoma model, and melanoma tumor growth re-commenced after stroma deterioration permitted xylitol solution leakage. These findings suggest that xylitol may have potential as an adjunct therapy in cancer treatment, specifically in melanoma. The differential response between the two cancer models underscores the complexity of cancer biology and the need for further investigation into xylitol's mechanisms of action and its role in cancer therapy.

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Cancer Cell Line Inhibition by Osmotic Pump-administered Xylitol in a Syngeneic Mouse Model

Cannon,

Dempsey,

Cosantino

et al. 2024

Preprint

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Background: This study aimed to evaluate the effects of continuous administration of xylitol (a commonly used dental prebiotic) via a subcutaneous osmotic minipump in a B16F10 syngeneic mouse model. Methods: The B16F10 syngeneic model consisted of 6–8-week-old C57BL/6 male mice subcutaneously injected with five × 105 B16F10 cells suspended in 100 μl PBS in the right flank. The mice were randomly assigned to two groups: Group 1 was the treatment group with 10% xylitol-loaded pumps (n=10), while Group 2 was the control group with saline-loaded pumps (n=10). Alzet minipumps were implanted subcutaneously in the left flank of B16F10-injected mice once more than 50% of all mice developed palpable tumors. After pump implantation surgery, the mice were monitored daily and weighed 2–3x/week. Tumor sizes were measured with calipers 2–3x/week, and all mice were euthanized when their tumors became too large (20 mm on any axis or 2,000 mm3). The excised tumors were weighed and cut in half, with one half sent for histology and the other for metabolomic analysis. Results: The xylitol-treated group survived substantially longer than the control group. The tumor size was reduced by approximately 35% by volume. Histological sections of xylitol treat mice suggested reduced infiltration and angiogenesis, which is consistent with previous studies. The metabolomic analysis demonstrates that xylitol reduces the tumor production of histamine, NADP+, ATP, and glutathione from the tumor, thereby improving the host immune response with ROS reactive oxygen species. Conclusions: The results of this study suggest that xylitol has potential as an adjunct to oncological treatment and is being further investigated in comparison to monoclonal antibody therapy (Opdualag).

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Partial Substitution of Glucose with Xylitol Prolongs Survival and Suppresses Cell Proliferation and Glycolysis of Mice Bearing Orthotopic Xenograft of Oral Cancer

Cited by 5 publications

References 39 publications

Salivary Transcriptome and Mitochondrial Analysis of ASD Children Compared to Healthy Controls

Salivary Transcriptome and Mitochondrial Analysis of ASD Children Compared to Healthy Controls

Effects of Xylitol on Tumor Progression in Syngeneic Mice Cancer Models

Cancer Cell Line Inhibition by Osmotic Pump-administered Xylitol in a Syngeneic Mouse Model

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