Participation of 5-HT3, 5-HT4, and nicotinic receptors in the peristaltic reflex of guinea pig distal colon

Kadowaki, Makoto; Wade, Paul R.; Gershon, Michael D.

doi:10.1152/ajpgi.1996.271.5.g849

Cited by 78 publications

(105 citation statements)

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“…Among others, synaptic transmission through nAChRs affects the initiation and/or propagation of the peristaltic reflex (Galligan and Burks, 1986;Kadowaki et al, 1996), the secretion of gastrin, somatostatin and gastric acid (Nishi et al, 1985;Cho et al, 1987;Wong and Ogle, 1995), the motility of the duodenal wall, and the secretion of fluids (Fandriks et al, 1994(Fandriks et al, , 1995.…”

Section: Nachrs In the Control Of Gut Functionmentioning

confidence: 99%

Nicotinic mechanisms in the autonomic control of organ systems

Biasi

2002

J. Neurobiol.

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Section: Nachrs In the Control Of Gut Functionmentioning

confidence: 99%

Nicotinic mechanisms in the autonomic control of organ systems

Biasi

2002

J. Neurobiol.

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“…A subset of myenteric interneurons is serotonergic (8,13,22,29,31,76,77); therefore, 5-HT antagonists can block peristaltic reflexes by inhibiting enteric serotonergic neurotransmission (46,60,82) as well as by interfering with the paracrine stimulation of IPANs. 5-HT from EC cells also plays a role in extrinsic sensation by stimulating extrinsic primary afferent nerves (2,39,40).…”

mentioning

confidence: 99%

5-HT_2Areceptors: location and functional analysis in intestines of wild-type and 5-HT_2Aknockout mice

Fiorica‐Howells¹,

Hen

Gingrich

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

106

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. 5-HT2A receptors: location and functional analysis in intestines of wild-type and 5-HT2A knockout mice. Am J Physiol Gastrointest Liver Physiol 282: G877-G893, 2002. First published January 9, 2002 10.1152/ajpgi.00435.2001.-The distribution and function of the 5-hydroxytryptamine (5-HT2A) receptor were investigated in the intestines of wild-type (5-HT2A ϩ/ϩ) and knockout (5-HT2A Ϫ/Ϫ) mice. In 5-HT2A ϩ/ϩ mice, rats, and guinea pigs, 5-HT2A receptor immunoreactivity was found on circular and longitudinal smooth muscle cells, neurons, enterocytes, and Paneth cells. Muscular 5-HT2A receptors were concentrated in caveolae; neuronal 5-HT2A receptors were found intracellularly and on the plasma membranes of nerve cell bodies and axons. Neuronal 5-HT2A immunoreactivity was detected as early as E14 in ganglia, intravillus nerves, and the deep muscle plexus. The 5-HT2A Ϫ/Ϫ colon did not express 5-HT2A receptors and did not contract in response to exogenous 5-HT. 5-HT2A Ϫ/Ϫ enterocytes were smaller, Paneth cells fewer, and muscle layers thinner (and showed degeneration) compared with those of 5-HT2A ϩ/ϩ littermates. The 5-HT2A receptor may thus be required for the maintenance and/or development of enteric neuroeffectors and other enteric functions, although gastrointestinal and colonic transit times in 5-HT2A Ϫ/Ϫ and ϩ/ϩ mice did not differ significantly. serotonin receptors; intestinal motility; immunocytochemistry THE ENTERIC NERVOUS SYSTEM (ENS) is different from the autonomic innervation of other organs, because it can mediate coordinated behaviors of the gut without central nervous system input (28,32,53). The presence in the bowel of intrinsic primary afferent neurons (IPANs) enables the ENS to respond to luminal stimuli. Because no nerve fibers enter the lumen, Bü lbring and Crema (7) proposed that sensory transduction is transepithelial, involving the pressure-induced secretion of 5-HT from enterochromaffin (EC) cells to stimulate the mucosal processes of the submucosal IPANs that initiate reflexes. This hypothesis has since been confirmed, and EC cell-derived 5-HT is now thought to activate both peristaltic (21,36,37,47,50,63) and secretory reflexes (12,73).In addition to its role in the initiation of enteric reflexes, 5-hydroxytryptamine (5-HT) also functions in ganglionic neurotransmission within the ENS. A subset of myenteric interneurons is serotonergic (8,13,22,29,31,76,77); therefore, 5-HT antagonists can block peristaltic reflexes by inhibiting enteric serotonergic neurotransmission (46, 60, 82) as well as by interfering with the paracrine stimulation of IPANs. 5-HT from EC cells also plays a role in extrinsic sensation by stimulating extrinsic primary afferent nerves (2, 39, 40).The bowel contains an abundance of 5-HT receptor subtypes located on neurons, smooth muscle, and epithelial cells (24,25,30). Enteric neuronal 5-HT receptors include 5-HT 1A (26,48,49,62), 5-HT 1P (6, 59, 64), 5-HT 2B (18), 5-HT 3 (15,25,42,59), and 5-HT 4 (36,37,62,80). Enteric members of the 5-HT 2 family are associated with s...

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“…29,36,49 In contrast, others demonstrated that nicotinic receptors are not essential in mediating peristalsis of the guinea pig distal colon. 50,51 They showed that hexamethonium temporarily arrested peristalsis and fecal pellet propulsion.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that the inserted pellets are well advanced distally in vitro preparations of the guinea pig distal colon, but not rat distal colon. [27][28][29] Our preliminary study also showed that the rat distal colon failed to propel fecal pellets in vitro (unpublished observations). Our laboratory has produced a number of studies in a rat model, demonstrating that transection of parasympathetic PN significantly delayed colonic transit time, while the transection of sympathetic hypogastric nerve significantly increased colonic motility.…”

Section: Discussionmentioning

confidence: 99%

“…28 Others showed that hexamethonium abolished the peristaltic reflex, suggesting the involvement of nicotinic receptors in the initiation of peristaltic reflex of the guinea pig colon. 29 In 1970, Crema et al 36 demonstrated that atropine partially inhibited peristaltic reflex in response to intraluminal balloon distension of the guinea pig distal colon. The authors explained that the inhibitory effect of atropine is due to preventing the descending relaxation, because the inhibitory neurons responsible for descending relaxation are regulated by muscarinic receptors.…”

Section: Discussionmentioning

confidence: 99%

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Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs

Gribovskaja‐Rupp¹,

Babygirija²,

Takahashi³

et al. 2014

J Neurogastroenterol Motil

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Background/AimsColonic peristalsis is mainly regulated via intrinsic neurons in guinea pigs. However, autonomic regulation of colonic motility is poorly understood. We explored a guinea pig model for the study of extrinsic nerve effects on the distal colon. MethodsGuinea pigs were sacrificed, their distal colons isolated, preserving pelvic nerves (PN) and inferior mesenteric ganglia (IMG), and placed in a tissue bath. Fecal pellet propagation was conducted during PN and IMG stimulation at 10 Hz, 0.5 ms and 5 V. Distal colon was connected to a closed circuit system, and colonic motor responses were measured during PN and IMG stimulation. ResultsPN stimulation increased pellet velocity to 24.6 ± 0.7 mm/sec (n = 20), while IMG stimulation decreased it to 2.0 ± 0.2 mm/sec (n = 12), compared to controls (13.0 ± 0.7 mm/sec, P ＜ 0.01). In closed circuit experiments, PN stimulation increased the intraluminal pressure, which was abolished by atropine (10 −6 M) and hexamethonium (10 −4 M). PN stimulation reduced the incidence of non-coordinated contractions induced by NG-nitro-L-arginine methyl ester (L-NAME; 10 −4 M). IMG stimulation attenuated intraluminal pressure increase, which was partially reversed by alpha-2 adrenoceptor antagonist (yohimbine; 10 −6 M). Conclusions

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Participation of 5-HT3, 5-HT4, and nicotinic receptors in the peristaltic reflex of guinea pig distal colon

Cited by 78 publications

References 0 publications

Nicotinic mechanisms in the autonomic control of organ systems

Nicotinic mechanisms in the autonomic control of organ systems

5-HT_2Areceptors: location and functional analysis in intestines of wild-type and 5-HT_2Aknockout mice

Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs

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Participation of 5-HT3, 5-HT4, and nicotinic receptors in the peristaltic reflex of guinea pig distal colon

Cited by 78 publications

References 0 publications

Nicotinic mechanisms in the autonomic control of organ systems

Nicotinic mechanisms in the autonomic control of organ systems

5-HT2Areceptors: location and functional analysis in intestines of wild-type and 5-HT2Aknockout mice

Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs

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Product

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5-HT_2Areceptors: location and functional analysis in intestines of wild-type and 5-HT_2Aknockout mice